Multispectral Hadamard Transform Raman Microscopy

Liu, Kei-Lee K.; Chen, Liheng; Sheng, Rongsheng; Morris, Michael D.

doi:10.1366/0003702914335175

Cited by 29 publications

(7 citation statements)

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“…Point measurements performed in these cellular investigations were aimed at characterizing structure and/or functional states of studied molecules in particular cellular organelles or domains. To measure the localization and continuous distribution of Raman scattering species along microspecimens, several approaches to micro-Raman image recording have been realized on a basis of narrow-band interference filtering (Puppels et al, 1993;Williams et al, 1994), tunable acoustooptic filtering (Treado et al, 1992), Hadamard multiplexing (Treado and Morris, 1990;Liu et al, 1991), and confocal scanning (Sharonov et al, 1994b;Sijtsema et al, 1998) techniques. The first three approaches are wide-field or global imaging methods that allow the acquisition of Raman data on a time-consuming point-by-point (or line-by-line) basis to be avoided, but at the expense of the absence of axial resolution.…”

Section: ϫ•mentioning

confidence: 99%

Confocal Raman Microspectroscopy and Imaging Study of Theraphthal in Living Cancer Cells

Feofanov

Grichine

Shitova

et al. 2000

Biophysical Journal

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Binary systems combining a transition metal complex and ascorbate have been proposed recently for catalytic therapy of malignant tumors. The killing effect on tumor cells is achieved by production of free radicals in the course of accelerated oxidation of ascorbate by dioxygen in the presence of transition metal complexes. Further progress in the development of binary catalytic systems (BCSs) requires a special method for their investigation in cells and tissues, because neither component of BCSs fluoresces. Here a resonance Raman confocal spectral imaging (RR CSI) technique was introduced as a unique approach to monitor quantitatively the transition metal complexes within living cells. Intracellular accumulation, localization, and retention of theraphthal (TP), a catalyst of the advanced TP/ascorbate BCS, were investigated in A549 cells with the RR CSI technique. The cellular analysis was complemented with the detailed study of molecular interactions of TP in solution and environmental factors affecting the RR spectrum of TP. TP does not penetrate into membranes, it binds very weakly to DNA and RNA, but it readily forms complexes with proteins. Binding with Ca(2+) cations and decreasing pH below 6 induce aggregation of TP. By analyzing RR spectra recorded from every point within a TP-treated cell, three states of the agent were discriminated, namely, monomeric TP in polar environment, TP bound to proteins, and aggregated TP. Their cytoplasmic and nuclear distributions were mapped at different stages of uptake and efflux. By introducing organelle-selective fluorescent probes into drug-treated cells and measuring intracellular localization of both the probe and the drug, compartmentation of TP was revealed. Cell growth suppression by the TP/ascorbate system was measured, and probable molecular and organelle targets of radical damage were characterized.

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Section: ϫ•mentioning

confidence: 99%

Confocal Raman Microspectroscopy and Imaging Study of Theraphthal in Living Cancer Cells

Feofanov

Grichine

Shitova

et al. 2000

Biophysical Journal

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“…In a recent series of papers Treado et al 9-H and Liu et al 12 described a Hadamard transform Raman microscope. This instrument also provides Raman images by means of image reconstruction.…”

Section: H a D A M A R D T R A N S F O R M R A M A N Microscopymentioning

confidence: 99%

Direct Imaging Raman Microscope Based on Tunable Wavelength Excitation and Narrow-Band Emission Detection

1993

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A new type of imaging Raman microscope is described. First the advantages and disadvantages of the two possible approaches to Raman microscopy based on signal detection by means of a charge-coupleddevice camera (i.e., direct imaging and image reconstruction) are discussed. Arguments are given to show that in most cases direct imaging is to be preferred over image reconstruction, because it provides the desired information in less time. In the direct imaging Raman microscope presented in this communication, detection of scattered light occurs in a narrow interval around a fixed wavelength. Selection of the Raman wavenumber shift at which an image is recorded is established by tuning the wavelength of the exciting laser light in such a way that the wavelength of the Raman scattered light with the desired Raman shift coincides with the detected wavelength. The microscope has been incorporated in a Raman microspectrometer in a way that enables easy switching between the imaging and the multichannel spectroscopy modes of operation. Bright field, fluorescence, and Raman microscopic images can be obtained.

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“…It has three advantages: (1) Fellgett's advantage, by which an improvement in the signal-to-noise ratio can be obtained; (2) the power distribution advantage; and (3) the source-encoded and signal-encoded image (10)(11)(12). In order to develop a new image analysis method for cellular quantitative analysis, in our previous studies, the technique of Hadamard transform multiplexed imaging was coupled with microscopy and a novel method for microscopic analysis was developed (13,14).…”

Section: Introductionmentioning

confidence: 99%

Analysis of DNA in Single Pollen Cell by Hadamard Transform Microscopic Fluorescence Imaging

Tang

Chen

Zeng

1999

Microchemical Journal

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Multispectral Hadamard Transform Raman Microscopy

Cited by 29 publications

References 1 publication

Confocal Raman Microspectroscopy and Imaging Study of Theraphthal in Living Cancer Cells

Confocal Raman Microspectroscopy and Imaging Study of Theraphthal in Living Cancer Cells

Direct Imaging Raman Microscope Based on Tunable Wavelength Excitation and Narrow-Band Emission Detection

Analysis of DNA in Single Pollen Cell by Hadamard Transform Microscopic Fluorescence Imaging

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