Analysis of DNA in Single Pollen Cell by Hadamard Transform Microscopic Fluorescence Imaging

This study presents a novel miniaturized Hadamard transform fluorescence imaging microscope, by combining a conventional fluorescence microscope with Hadamard transform multiplexing encoding using a one-dimensional movable mask to realize spatial resolution and a linear CCD for multichannel detection. The microscope can provide high-resolution automatically-generated 0-255 gray level HT images for morphological analysis and visualization of a single cell, and normalized HT images for cellular quantitative measurements. The microscope's imaging capability was applied to measure the DNA content in human lymphocyte, chicken erythrocyte and eel erythrocyte, and a comparative study was performed. The results show that the calibrated DNA content in a chicken erythrocyte is 2.32 pg when human lymphocyte is used as the standard, and eel erythrocyte may be a potentially reliable and novel standard for determining DNA contents in other species because it has a stable DNA value of 2.06 pg, with a CV of 4.3% when 20 eel erythrocytes are measured. The results also demonstrate that the HT imaging microscope should be valuable in the fields of medicine and cell biology.

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High-resolution Hadamard transform microscope fluorescence imaging: quantifying the DNA content in single cells

Tang

Luo

Xiong

et al. 2005

Anal Bioanal Chem

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Hadamard transform fluorescence image microscopy using one-dimensional movable mask

Tang¹,

Chen²,

Zhou³

et al. 2002

Analytica Chimica Acta

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Single‐Cell Analysis in a Plastic Microfluidic Channel with a Hadamard Transform Microscopic Fluorescence Image System

Tang

Luo

et al. 2004

Analytical Letters

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Analysis of DNA in Single Pollen Cell by Hadamard Transform Microscopic Fluorescence Imaging

Cited by 7 publications

References 12 publications

High-resolution Hadamard transform microscope fluorescence imaging: quantifying the DNA content in single cells

High-resolution Hadamard transform microscope fluorescence imaging: quantifying the DNA content in single cells

Hadamard transform fluorescence image microscopy using one-dimensional movable mask

Single‐Cell Analysis in a Plastic Microfluidic Channel with a Hadamard Transform Microscopic Fluorescence Image System

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