Hadamard transform fluorescence image microscopy using one-dimensional movable mask

Virtual Column Method for Correcting Masking Effects in Hadamard Transform Systems

“…To reveal phenomena inside a living cell, biologists have needed an optical microscope with a m uch higher resolution beyond the diffraction limit. 6 Under this circumstance, we have proved that a uorescence depletion process induced by two-color irradiation (two-color uorescence dip spectroscopy) is able to break the resolution limit of a laser scanning microscope. 7 Figure 1 shows the excitation scheme of two-color uorescence dip spectroscopy.…”

Applications of Hadamard Transform-Gas Chromatography/Mass Spectrometry (HT-GC/MS) to the Detection of Pesticides in Rice

“…The Hadamard transform (HT) technique has been applied in a variety of fields, including time-of-flight mass spectrometry (Brock et al, 1998;Fernández et al, 2002;Trapp et al, 2004;Fernández et al, 2001), Raman spectrometry (Treado et al, 1990;DeVerse et al, 2000;DeVerse et al, 1999), fluorescence imaging (Chen et al, 1995;Mei et al, 1996;Tang et al, 2002;Hassler et al, 2005) ion mobility spectrometry (Clowers et al, 2006;Szumlas et al, 2006), and NMR (Kubo et al, 1996;Feliz et al, 2006). In addition, the application of the cross correlation technique to chromatographic separation was first proposed by Izawa and coworkers.…”

Application of Hadamard transform to gas chromatography/nonresonant multiphoton ionization/time‐of‐flight mass spectrometry

“…The chromatogram was decoded by means of inverse Hadamard transformation, and as a result, the S / N ratios were substantially improved, compared with a conventional single injection method. In fact, the HT technique has been applied in many fields, including TOFMS 14–17, Raman spectrometry 18–20, fluorescence imaging 21–24, ion mobility spectrometry 25, 26 and even NMR 27, 28. This technique has also been successfully applied to CE separations, where the theoretical background and experimental results were presented to demonstrate the capabilities and analytical advantages of the HT technique 29–33.…”