Raman Spectroscopic Measurement of Spatial and Temporal Temperature Gradients in Operating Electrophoresis Capillaries

Liu, Kei-Lee K.; Davis, Kevin L.; Morris, Michael D.

doi:10.1021/ac00093a033

Cited by 85 publications

(60 citation statements)

References 25 publications

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“…6A) for D,L-Dns-Ala and 36.071.31C (Fig. 6B) for D,L-Dns-Asn, respectively, when the ambient air temperature was 20.01C, which was similar to the case mentioned in reference [28]. These results indicated that the non-spectroscopic approach to determining temperature in CLE-CE was available.…”

Section: Determination Of the Temperature In Capillarysupporting

confidence: 79%

“…Therefore, the reliable use of CE required a means of accurately measuring the temperature inside the capillary during the course of CE. Temperature in capillaries was usually measured with a number of spectroscopic techniques, such as backscattering of light [27], Raman spectroscopy [28] of hydrogen bonds. The developed CLE-CE method combining on-column labeling of D,L-AAs with Dns-Cl and the CE instrument (P/ACE model 5000) has been explored for studying the dependence of migration time on temperature.…”

Section: Determination Of the Temperature In Capillarymentioning

confidence: 99%

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On‐column labeling technique and chiral ligand‐exchange CE with zinc(II)‐L‐arginine complex as a chiral selector for assay of dansylated D,L‐amino acids

Yang

2009

Electrophoresis

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A novel on-column labeling method of amino acid (AA) enantiomers by using dansyl chloride (Dns-Cl) has been explored combined with chiral ligand-exchange CE (CLE-CE) technique and UV detection. Efficient labeling was achieved by sequential injection of buffer, Dns-Cl, AA enantiomers, Dns-Cl and buffer at 0.2 psi for 10.0, 3.0, 24.0, 3.0, and 10.0 s, respectively. After injection, the sandwich sections were allowed to react at room temperature for 35.0 min. With this procedure, successful on-column labeling and CLE-CE separation of 17 pairs AA enantiomers have been achieved with a buffer of 100.0 mM boric acid, 5.0 mM ammonium acetate, 3.0 mM ZnSO4 and 6.0 mM L-Arg at pH 8.4, giving nine pairs fully enantioresolved with resolution in between 2.0 and 5.1. CLE-CE of some individual and mixed pairs was also demonstrated, much the same as using pre-column labeling. As validated by both artificially prepared solutions and serum samples, this new method was shown to be applicable to the quantitative analysis, with a linear range between 14.0 muM and 3.7 mM, correlation coefficient above 0.99 and recovery in between 90.4% and 111.7%. It was also demonstrated that the migration time-temperature based curve allows for temperature determination in CE by using this new method.

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Section: Determination Of the Temperature In Capillarysupporting

confidence: 79%

Section: Determination Of the Temperature In Capillarymentioning

confidence: 99%

On‐column labeling technique and chiral ligand‐exchange CE with zinc(II)‐L‐arginine complex as a chiral selector for assay of dansylated D,L‐amino acids

Yang

2009

Electrophoresis

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“…Silicon also represents a good masking material by adhering better to glass than gold. It is also resistant to concentrated hydrogen fluoride [26]. Usually used etching mediums contain various acidic solutions, HF/HNO 3 /H 2 O being the most commonly used [23,[27][28][29][30][31][32].…”

Section: Glass Wafermentioning

confidence: 99%

New advances in microchip fabrication for electrochromatography

Szekeres

Guttman

2005

Electrophoresis

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There is a great demand in separation technologies for faster and more effective analysis processes. Miniaturization is a suitable technique for satisfying this demand as reduction in size gives increased separation speed with higher efficiency. CEC is an electric-field-mediated separation technique where the liquid flow is generated by the electric field itself. The main advantage of using electric field over pressure for flow generation is the flat flow profile of the EOF; thus, CEC is one of the best candidates to construct a novel and high-efficiency microanalytical device. The aim of the present paper is to review the basic fabrication and bonding principles, as well as connection and system integration options for microfluidics-based electrochromatography. The physical structure and fluidic channel formation are critically evaluated, including glass microstructuring and fusion bonding. Recent developments in nanoflow measurements and the application of various flow control units are also extensively discussed.

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“…8, the temperature predictions from the simple model using the working parameters in the studies by Liu et al [31] and Petersen et al [18] are shown. In the study by Liu et al [31], the measured steady temperature under free-air cooling was about 587C. In Fig.…”

Section: Comparison Between Simple Model and Experimental Measurementmentioning

confidence: 88%

Estimation of Joule heating effect on temperature and pressure distribution in electrokinetic‐driven microchannel flows

2006

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In this study we present simple analytical models that predict the temperature and pressure variations in electrokinetic-driven microchannel flow under the Joule heating effect. For temperature prediction, a simple model shows that the temperature is related to the Joule heating parameter, autothermal Joule heating parameter, external cooling parameter, Peclet number, and the channel length to channel hydraulic diameter ratio. The simple model overpredicted the thermally developed temperature compared with the full numerical simulation, but in good agreement with the experimental measurements. The factors that affect the external cooling parameters, such as the heat transfer coefficient, channel configuration, and channel material are also examined based on this simple model. Based on the mass conservation, a simple model is developed that predicts the pressure variations, including the temperature effect. An adverse pressure gradient is required to satisfy the mass conservation requirement. The temperature effect on the pressure gradient is via the temperature-dependent fluid viscosity and electroosmotic velocity.

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Raman Spectroscopic Measurement of Spatial and Temporal Temperature Gradients in Operating Electrophoresis Capillaries

Cited by 85 publications

References 25 publications

On‐column labeling technique and chiral ligand‐exchange CE with zinc(II)‐L‐arginine complex as a chiral selector for assay of dansylated D,L‐amino acids

On‐column labeling technique and chiral ligand‐exchange CE with zinc(II)‐L‐arginine complex as a chiral selector for assay of dansylated D,L‐amino acids

New advances in microchip fabrication for electrochromatography

Estimation of Joule heating effect on temperature and pressure distribution in electrokinetic‐driven microchannel flows

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