New advances in microchip fabrication for electrochromatography

Szekeres, L.; Guttman, András

doi:10.1002/elps.200500487

Cited by 55 publications

(33 citation statements)

References 151 publications

(206 reference statements)

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“…On the other hand, MEKC on microchips has fully met the expectations anticipated in the previous review. Thus, microfluidic devices have received much attention over the last four years, and a number of systems that can perform analytical procedures involving chemical reactions, separation, and detection on a single microchip have been developed [20,21]. Although MEKC can be implemented on chip devices, they have been primarily designed for CZE, so they are not included in this review either.…”

Section: Introductionmentioning

confidence: 99%

MEKC: An update focusing on practical aspects

Silva

2007

Electrophoresis

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This paper reviews recent methodological and instrumental advances in MEKC. Improvements in sensitivity arising from the use of on-line sample concentration (sweeping, stacking, and combination of both protocols) and derivatization (in-capillary reactions and coupling with flow-injection systems) and improvements in resolution obtained by changing the composition of the BGE (e.g., with organic modifiers, ionic liquids, nonionic and zwitterionic surfactants, mixed micelles, and vesicles) or using coated capillaries are discussed in detail. In addition, MS and LIF spectroscopy are examined in relation to their advantages and restrictions as applied to MEKC analysis. Some thoughts on potential future directions are also expressed.

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Section: Introductionmentioning

confidence: 99%

MEKC: An update focusing on practical aspects

Silva

2007

Electrophoresis

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“…Lab-on-a-chip devices have the potential to revolutionize chemical and biological analysis procedures [1][2][3]. The CE technique is commonly employed to separate ions in a sample since it has a low sample consumption and a good detection resolution [4,5].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of semicircular detection electrodes for contactless conductivity detector – CE microchips

et al. 2006

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This study uses simple and reliable microfabrication techniques to fabricate CE biochips, integrating a novel contactless conductivity detector in a miniaturized detection system in a microfluidic biochip. The off-channel electrodes are deposited around side channels by Au sputtering and patterned using a standard "lift-off" process. A vacuum fusion bonding process is employed to seal the lower substrate containing the microchannels and the electrodes to an upper glass cover plate. The variations in the capacitance between the semicircular detection electrodes in the side channels are measured as different samples and ions pass through the detection region of the CE separation channel. Samples of Rhodamine B, commercial sports drinks, mineral waters, and a red wine, respectively, are mixed in different buffer solutions, separated, and successfully detected using the developed device. The semicircular detection electrodes for the contactless conductivity detector have microscale dimensions and provide a valuable contribution to the realization of the lab-on-a-chip concept.

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“…18,19,42,99,100 The EOF, as a stateof-the-art approach widely applied in miniaturized systems for fluid delivery, offers many advantageous features. The EOF can be induced by applying an external electric field parallel to a surface with a net surface charge density to drive the bulk fluid.…”

Section: Control Of Microfluidic Flow By Functional Phospholipid Polymentioning

confidence: 99%

Phospholipid Polymer Biointerfaces for Lab-on-a-Chip Devices

Takai

Ishihara

2010

Ann Biomed Eng

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This review summarizes recent achievements and progress in the development of various functional 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer biointerfaces for lab-on-a-chip devices and applications. As phospholipid polymers, MPC polymers can form cell-membrane-like surfaces by surface chemistry and physics and thereby provide biointerfaces capable of suppressing protein adsorption and many subsequent biological responses. In order to enable application to microfluidic devices, a number of MPC polymers with diverse functions have been specially designed and synthesized by incorporating functional units such as charge and active ester for generating the microfluidic flow and conjugating biomolecules, respectively. Furthermore, these polymers were incorporated with silane or hydrophobic moiety to construct stable interfaces on various substrate materials such as glass, quartz, poly(methyl methacrylate), and poly(dimethylsiloxane), via a silane-coupling reaction or hydrophobic interactions. The basic interfacial properties of these interfaces have been characterized from multiple aspects of chemistry, physics, and biology, and the suppression of nonspecific bioadsorption and control of microfluidic flow have been successfully achieved using these biointerfaces on a chip. Further, many chip-based biomedical applications such as immunoassays and DNA separation have been accomplished by integrating these biointerfaces on a chip. Therefore, functional phospholipid polymer interfaces are promising and useful for application to lab-on-a-chip devices in biomedicine.

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New advances in microchip fabrication for electrochromatography

Cited by 55 publications

References 151 publications

MEKC: An update focusing on practical aspects

MEKC: An update focusing on practical aspects

Fabrication and characterization of semicircular detection electrodes for contactless conductivity detector – CE microchips

Phospholipid Polymer Biointerfaces for Lab-on-a-Chip Devices

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