The adverse effects of antibiotics have attracted widespread attention, thus reducing the use of antibiotics in animal feed has become a very important issue in improving of the health of livestock. The effects of
Enterococcus faecium
(
E. faecium
) on growth performance and gut microbiota in weaned piglets were investigated in the present study. Piglets were randomly assigned to four treatments: a control group fed with a diet containing 75 mg/kg aureomycin (Diet 1 group) and three experimental groups fed with diets of 50 mg/kg aureomycin (Diet 2 group), 50 mg/kg aureomycin + 9 × 10
5
CFU/g
E. faecium
(Diet 3 group), or 50 mg/kg aureomycin + 1.2 × 10
6
CFU/g
E. faecium
(Diet 4 group). Their gut microbial communities were analyzed by sequencing the V3–V4 region of the 16SrRNA gene. The results showed that the final body weights and the average daily gain of the weaned piglets in the Diet 2 group were higher (
P
= 0.05) than those in the Diet 1 or Diet 3 group. Decreasing trends (
P
= 0.08) was observed in mortality rate in the Diet 3 and 4 group when compared with that in the Diet 1 group. Increases in the Sobs, Chao1, ACE, and Shannon indexes and a decrease in the Simpson index were observed at intervals from day 1 to 14 (
P
< 0.05). The Sobs, Chao1, and ACE indexes in the Diet 3 group were the lowest on day 14 (
P
< 0.05). The abundance of
Bacteroidetes
was increased and that of
Proteobacteria
was decreased from day 1 to 7, but both of them kept stable from day 7 to 14. Besides, the lowest abundance of
Fusobacteria
,
Lentisphaerae
, and
Planctomycetes
was observed on day 1 and the lowest abundance of
Actinobacteria
was observed on day 14 in the Diet 3 group (
P
< 0.05). Overall, these results suggest that the antibiotics and
E. faecium
interventions result in different changes in the gut microbiota, and a reduced antibiotics diet supplemented with 1.2 × 10
6
CFU/g
E. faecium
does not affect the growth performance in weaned piglets.
Electronic supplementary material
The online version of this article (10.1186/s13568-019-0755-z) contains supplementary material, which is available to authorized users.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.