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The murine cell surface antigen mCD156 is a glycoprotein that is expressed in monocytic cell lines and consists of a metalloprotease domain, a disintegrin domain, a cysteine-rich domain, and an epidermal growth factorlike domain in the extracellular region. The mCD156 gene is composed of 24 exons and 23 introns and spans approximately 14 kilobases. The first exon encodes most of the signal peptide sequence, and the transmembrane region is encoded by a single exon (19). In contrast, the other regions are composed of multiple exons. Of these, exons 7-12 and 12-15 encode a metalloprotease domain and a disintegrin domain, respectively. Sequence analysis of the 5-flanking DNA revealed many potential regulatory motifs. Chloramphenicol acetyltransferase analysis demonstrated that nucleotides at positions ؊183, ؊334, and ؊623 contained cis-acting enhancing elements in a mouse monocytic cell line, aHINS-B3. Nucleotides at positions ؊183 and ؊390 contained elements responsible for lipopolysaccharide (LPS) inducibility, although several other 5-flanking regions were also involved in LPS responsiveness. Regions ؊202, ؊507, and ؊659 play a role in interferon-␥ inducibility. Some of the potential regulatory motifs and other unknown cis elements may be involved in the constitutive expression, and LPS and interferon-␥ inducibilities. The mCD156 gene was mapped to chromosome 7, region F3-F4.The mouse CD156 (mCD156) 1 (MS2) and human CD156 are type I transmembrane glycoproteins found on myelomonocytic cell lineage (1, 2). The mCD156 cDNA is 3.2 kb long with an open reading frame encoding 826 amino acids. The extracellular region consisting of 644 amino acids has a metalloprotease (MTP) domain containing the zinc-binding consensus histidine and protease catalytic glutamic residues and a disintegrin domain containing a platelet aggregation inhibitor-like structure, whereas the cytoplasmic region consisting of 143 amino acids contains a proline-rich amino acid sequence containing consensus SH3 (Src homology 3) binding sequence. mCD156 plays a role in infiltration of leukocytes by leukocyte adhesion to en-

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