CD156 (Human ADAM8): Expression, Primary Amino Acid Sequence, and Gene Location

Yoshiyama, Kazuhiro; Higuchi, Yasunori; Kataoka, Masashi; Matsuura, Keiko; Yamamoto, Shunsuke

doi:10.1006/geno.1997.4607

Cited by 99 publications

(81 citation statements)

References 33 publications

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“…We localized the human CD156 gene to the distal region of chromosome 10q26.3 (2), an area syntenic to mouse chromosome 7, region F3-F4, the region of chromosome 7 on which the mCD156 gene is found. ADAM 11 has been mapped to human chromosome 17q21 (12), where many genes including the myeloperoxydase and homeo box region 2 genes are mapped (47,48).…”

Section: Resultsmentioning

confidence: 99%

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Structure of the Murine CD156 Gene, Characterization of Its Promoter, and Chromosomal Location

Kataoka

Yoshiyama

Matsuura

et al. 1997

Journal of Biological Chemistry

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The murine cell surface antigen mCD156 is a glycoprotein that is expressed in monocytic cell lines and consists of a metalloprotease domain, a disintegrin domain, a cysteine-rich domain, and an epidermal growth factorlike domain in the extracellular region. The mCD156 gene is composed of 24 exons and 23 introns and spans approximately 14 kilobases. The first exon encodes most of the signal peptide sequence, and the transmembrane region is encoded by a single exon (19). In contrast, the other regions are composed of multiple exons. Of these, exons 7-12 and 12-15 encode a metalloprotease domain and a disintegrin domain, respectively. Sequence analysis of the 5-flanking DNA revealed many potential regulatory motifs. Chloramphenicol acetyltransferase analysis demonstrated that nucleotides at positions ؊183, ؊334, and ؊623 contained cis-acting enhancing elements in a mouse monocytic cell line, aHINS-B3. Nucleotides at positions ؊183 and ؊390 contained elements responsible for lipopolysaccharide (LPS) inducibility, although several other 5-flanking regions were also involved in LPS responsiveness. Regions ؊202, ؊507, and ؊659 play a role in interferon-␥ inducibility. Some of the potential regulatory motifs and other unknown cis elements may be involved in the constitutive expression, and LPS and interferon-␥ inducibilities. The mCD156 gene was mapped to chromosome 7, region F3-F4.The mouse CD156 (mCD156) 1 (MS2) and human CD156 are type I transmembrane glycoproteins found on myelomonocytic cell lineage (1, 2). The mCD156 cDNA is 3.2 kb long with an open reading frame encoding 826 amino acids. The extracellular region consisting of 644 amino acids has a metalloprotease (MTP) domain containing the zinc-binding consensus histidine and protease catalytic glutamic residues and a disintegrin domain containing a platelet aggregation inhibitor-like structure, whereas the cytoplasmic region consisting of 143 amino acids contains a proline-rich amino acid sequence containing consensus SH3 (Src homology 3) binding sequence. mCD156 plays a role in infiltration of leukocytes by leukocyte adhesion to en-

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Section: Resultsmentioning

confidence: 99%

“…The mouse CD156 (mCD156) 1 (MS2) and human CD156 are type I transmembrane glycoproteins found on myelomonocytic cell lineage (1,2). The mCD156 cDNA is 3.2 kb long with an open reading frame encoding 826 amino acids.…”

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confidence: 99%

Structure of the Murine CD156 Gene, Characterization of Its Promoter, and Chromosomal Location

Kataoka

Yoshiyama

Matsuura

et al. 1997

Journal of Biological Chemistry

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“…Human ADAM8 has been clustered as a human leukocyte differentiation antigen, CD156, and is thought to play a role in cell adhesion and infiltration of inflammatory cells [23,24]. ADAM8 was expressed in bronchial and alveolar type II epithelium and interstitial inflammatory cells, predominantly with morphology of neutrophils and macrophages, without smooth muscle expression.…”

Section: Discussionmentioning

confidence: 99%

Expression of ADAMs (“a disintegrin and metalloprotease”) in the human lung

et al. 2009

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In view of the associations of "a disintegrin and metalloprotease" (ADAM) with respiratory diseases, we assessed the expression of various ADAMs in human lung tissue. Lung tissue was obtained from nine individuals who underwent surgery for lung cancer or underwent lung transplantation for emphysema. Also, 16HBE 14o-(human bronchial epithelial) and A549 (alveolar type II epitheliumlike) cell lines were used. Immunohistochemistry was performed with antibodies recognizing different ADAM domains. The ADAMs were typically distributed over the bronchial epithelium. ADAM8 and ADAM10 were expressed diffusely in all layers of the epithelium. ADAM9, ADAM17, and ADAM19 were predominantly expressed in the apical part of the epithelium, and ADAM33 was predominantly and strongly expressed in basal epithelial cells. In smooth muscle, ADAM19 and ADAM17 were strongly expressed, as was ADAM33, though this expression was weaker. ADAM33 was strongly expressed in vascular endothelium. All ADAMs were generally expressed in inflammatory cells. The typical distribution of ADAMs in the lung, especially in the epithelium, is interesting and suggests a localized function. As most ADAMs are involved in release of (pro-) inflammatory mediators and growth factors, they may play an important role in the first line of defense and in initiation of repair events in the airways.

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“…ADAM8 is a transmembrane glycoprotein restricted to granulocytes and monocytic cells, playing a role in collagen degradation in extravascular tissue. It is also involved in leukocyte extravasation functioning as a ligand for integrins (50). CCL2, secreted by adipocytes and endothelial cells, is a potent macrophage-attracting chemokine, the expression of which is increased in obese human adipose tissue (9,34).…”

Section: Discussionmentioning

confidence: 99%