An experiment was carried out to study the hematobiochemical changes of chronic lead poisoning in adult male wistar albino rats for a period of twelve weeks. Adult 216 healthy rats were randomly divided into six groups viz. control (groupI), higher dose of lead acetate @60mgs/kg bwt (group II), Lower dose of lead acetate @ 30 mgs / kg bwt (group III), Higher dose of lead + Ocimum @ 400 mgs/ kg bwt (group IV), lower dose of lead + Ocimum @ 400 mgs/ kg bwt (group V), Ocimum control (group VI). All lead treated and ameliorated groups given Lead acetate/ lead + Ocimum orally for three days in a week for a period of twelve weeks. The mean PCV, Hb, values were reduced significantly (P<0.05) in lead treated rats as dose dependent manner. Where as significant improvement was noticed in Ocimum treated groups Increased TLC and PLC values as dose dependent manner. A significant reduction in PNC was noticed in ocimum treated groups. Significant (P<0.05) decrease in serum total protein values, serum glucose and increased creatinine values were observed in lead treated groups as dose dependent . Increased protein & glucose and decreased creatinine values obtained in Ocimum treated groups. The alterations in hematological and biochemical parameters in the present study indicates decreased lifespan & fragility of RBC and damage to liver, kidney and Pancreas in lead poisoned wistar albino rats.
Lymphoid tumors comprise one of the most common groups of tumors in domestic animals. Lymphosarcoma is expressed as a specific multicentric form in calvesupto six months of age (sporadic) and in adult cattle (enzootic); and as thymic form in young cattle. Eight calves of both sexes aged below six months were necropsied in the department of veterinary Pathology, college of Veterinary Science, Tirupati. During necropsy impression smears, represented tissue samples were collected for Cytopathology, histopathology, AgNOR count and immunohistochemical studies. Cytological smears revealed lymphocytes, prolymphocytes and lymphoblasts with anaplastic changes. Histopathologically, partial to complete obliteration of architecture due to the replacement of normal tissue by lymphoblastic cells in lymphnodes and other visceral organs. The mean AgNOR count of the lymphosarcoma was 4.25 and it appear as a round to irregular, dark brown to black dots of varying sizes in the nuclei. Positive expression of PCNA and Ki67 was noticed in lymphnode, liver, kidney and lung was observed. The labeling index of PCNA and Ki-67 expression and distribution of immunopositive cells may be helpful in the early diagnosis and prognosis of malignant tumors. Hence, AgNOR, PCNA and Ki-67 indices can be used as reliable markers of cell proliferation and malignancy.
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