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^Äëíê~Åí= Optimal conditions for pilot-scale production of the carboxymethylcellulase (CMCase) by _~Åáääìë=~ãóäçäáèìÉÑ~ÅáÉåë DL-3 were investigated. The best carbon and nitrogen sources for the production of CMCase by _K=~ãóäçäáèìÉÑ~ÅáÉåë DL-3 were found to be rice hull and peptone and their optimal concentrations were 5.0 and 0.20% (w/v), respectively. Optimal temperature and initial pH for the production of CMCase were 37°C and 6.8. Optimal agitation speed and aeration rate for the production of CMCase were 300 rpm and 1.0 vvm in a 7 L bioreactor, which were different from those for the cell growth of _K= ãóäçäáèìÉÑ~ÅáÉåë DL-3. The highest productions of CMCase by _K=~ãóäçäáèìÉÑ~ÅáÉåë DL-3 from 5.0% (w/v) rice hull as a carbon source under optimal conditions in a 7 or 100 L bioreactor were 220 and 367 U/mL, respectively. © KSBB

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