Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3

Jo, Kang-Ik; Lee, You-Jung; Kim, Bokyung; Lee, Bo-Hwa; Chung, Chong-Pyoung; Nam, Soo-Wan; Kim, Sung-Koo; Lee, Jin‐Woo

doi:10.1007/s12257-007-0149-y

Cited by 54 publications

(48 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The conversion rate of gellan from 20 g/l glucose was 43%. Potassium phosphate is one of major salts in a medium for the production of microbial polysacchrides and enzymes [14,15,29] as well as a well-known ingredient in buffer solutions [20,26]. Production of gellan by S. paucibilis NK2000 under pH control by potassium phosphate as well as sodium hydroxide was higher than that without pH control.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Potassium Phosphate as a pH Stabilizer on the Production of Gellan by Sphingomonas paucibilis NK-2000

Lee¹,

Jo²,

Jin³

et al. 2009

Journal of Life Science

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

The Effect of Potassium Phosphate as a pH Stabilizer on the Production of Gellan by Sphingomonas paucibilis NK-2000

Lee¹,

Jo²,

Jin³

et al. 2009

Journal of Life Science

View full text Add to dashboard Cite

“…Dry cells weight (DCW) was measured as described in the previous report [10]. Activities of the CMCase produced by the isolated microorganism were determined based on the release of reducing sugar from CMC using the 3,5-dinitrosalicylic acid (DNS) method, as described in the previous report [14].…”

Section: Methodsmentioning

confidence: 99%

Enhanced Production of Carboxymethylcellulase by a Newly Isolated Marine Microorganism Bacillus atrophaeus LBH-18 Using Rice Bran, a Byproduct from the Rice Processing Industry

Kim¹,

Cao²,

Lee³

et al. 2012

Journal of Life Science

Self Cite

View full text Add to dashboard Cite

A microorganism producing carboxymethylcellulase (CMCase) was isolated from seawater and identified as Bacillus atrophaeus. This species was designated as B. atrophaeus LBH-18 based on its evolutionary distance and the phylogenetic tree resulting from 16S rDNA sequencing and the neighbor-joining method. The optimal conditions for rice bran (68.1 g/l), peptone (9.1 g/l), and initial pH (7.0) of the medium for cell growth was determined by Design Expert Software based on the response surface method; conditions for production of CMCase were 55.2 g/l, 6.6 g/l, and 7.1, respectively. The optimal temperature for cell growth and the production of CMCase by B. atrophaeus LBH-18 was 30℃.The optimal conditions of agitation speed and aeration rate for cell growth in a 7-l bioreactor were 324 rpm and 0.9 vvm, respectively, whereas those for production of CMCase were 343 rpm and 0.6 vvm, respectively. The optimal inner pressure for cell growth and production of CMCase in a 100-l bioreactor was 0.06 MPa. Maximal production of CMCase under optimal conditions in a 100-l bioreactor was 127.5 U/ml, which was 1.32 times higher than that without an inner pressure. In this study, rice bran was developed as a carbon source for industrial scale production of CMCase by B. atrophaeus LBH-18. Reduced time for the production of CMCase from 7 to 10 days to 3 days by using a bacterial strain with submerged fermentation also resulted in increased productivity of CMCase and a decrease in its production cost.

show abstract

“…Dry cells weight was measured as described in the previous report [12]. Activity of the CMCase produced by E. coli JM109/DL-3 was determined based on the release of reducing sugar from CMC using the 3,5-dinitrosalicylic acid (DNS) method, as described in the previous report [14].…”

Section: Methodsmentioning

confidence: 99%

“…Many studies on types of strains, culture conditions, and substrates for production of cellulases have been reported [12,15]. However, there have been few reports on optimization of mineral salts in the medium for production of cellulases.…”

Section: Most Commercial Cellulases Have Been Produced Bymentioning

confidence: 99%

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Lee¹,

Kim²,

Gao³

et al. 2011

Journal of Life Science

Self Cite

View full text Add to dashboard Cite

The optimal concentrations of salts in medium for cell growth and the production of carboxymethylcellulase (CMCase) by a recombinant E. coli JM109/DL-3 were established using two statistical methods: orthogonal array method (OAM) and response surface method (RSM). The analysis of variance (ANOVA) of data based on OAM indicated that K2HPO4 gave maximum sum of square (S) and percentage contribution (P) for cell growth as well as production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 in medium for cell growth extracted by Qualitek-4 (W32b) Software were 10.0, 1.0, 0.2, and 0.6 g/l, respectively, whereas those for the production of CMCase by E. coli JM109/DL-3 were 5.0, 1.0, 0.4, and 0.6 g/l. The analysis of variance (ANOVA) resulting from RSM indicated that a highly significant salt for cell growth was K2HPO4 ("probe>F" less than 0.0001), whereas K2HPO4 and MgSO4•7H2O were significant for the production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 for cell growth extracted by Design Expert Software were 7.44, 1.08, 0.22, and 0.88 g/l, respectively, whereas those for production of CMCase were 5.84, 0.69, 0.28, and 0.54 g/l. The optimal concentrations of salts and their influences on cell growth and production of CMCase extracted by OAM were almost the same as those by RSM. Production of CMCase by a recombinant E. coli JM109/DL-3 under optimized concentration of salts was 1.93 times higher than that by Bacillus amyloliquifaciens DL-3.

show abstract

Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3

Cited by 54 publications

References 26 publications

The Effect of Potassium Phosphate as a pH Stabilizer on the Production of Gellan by Sphingomonas paucibilis NK-2000

The Effect of Potassium Phosphate as a pH Stabilizer on the Production of Gellan by Sphingomonas paucibilis NK-2000

Enhanced Production of Carboxymethylcellulase by a Newly Isolated Marine Microorganism Bacillus atrophaeus LBH-18 Using Rice Bran, a Byproduct from the Rice Processing Industry

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Contact Info

Product

Resources

About