No abstract

Bovine norovirus (BoNoV) is an important cause of diarrhea in calves and has been reported in several countries. The aims of this study were to investigate for the first time the presence of norovirus in Turkish calves by real-time reverse transcription-polymerase chain reaction (qRT-PCR) and to determine the phylogeny of any circulating strains. Fecal samples from 70 diarrheic calves were collected and analysed by SYBR Green qRT-PCR. BoNoV was detected in fecal samples from six calves. The capsid gene was partially sequenced, and phylogenetic analysis was performed. This showed that the six Turkish BoNoVs clustered with the GIII-2 prototype.

No abstract

We investigated the prevalence of contamination of retail-level beef, mutton, and broiler carcasses in Istanbul, Turkey, by thermophilic Campylobacter spp. (TCS) and determined antimicrobial susceptibilities of the TCS strains isolated from these carcasses. From November 2005 to October 2006, 198 beef and 120 mutton carcass excision samples, and 232 chicken carcasses (whole and pieces) were randomly collected from different retail stores and meat-processing plants in Istanbul. TCS were isolated from 11.1%, 21.6%, and 50.4% of beef, mutton, and chicken samples tested, respectively. There was no significant seasonal variation in the prevalence of TCS. A total of 292 Campylobacter isolates were obtained from the samples (56.5% C. jejuni, 33.9% C. Coli, and 9.6% C. lari). C. jejuni was the species most commonly isolated from chicken meat (56.5%<65.9 from table 2>), while C. coli was the most common in beef (63.3%) and mutton (63.9%) carcasses. Susceptibilities of 246 strains for eight antimicrobial drugs were determined using disk diffusion assay. Campylobacter isolates were most often resistant to tetracycline (69.1%), followed by trimethoprim-sulfamethoxazole (64.2%), nalidixic acid (58.1%), erythromycin (56.9%), enrofloxacin (48.8%), ciprofloxacin (42.7%), chloramphenicol (36.2%), and gentamicin (26.0%). The results of this study suggest that a high proportion of meat samples, particularly chicken carcasses, are contaminated by TCS, most of which are antimicrobial-resistant strains. Since campylobacteriosis is transmitted primarily through food of animal origin, the presence of antimicrobial-resistant strains in meat has important implications for public health and food safety.

This study was carried out to investigate the antimicrobial effects of chopped garlic in ground beef and raw meatball (çig köfte), which is a traditional food product eaten raw. Fresh minced ground beef and raw meatball batter prepared with traditional methods were separated into groups. Chopped and crushed garlic was added to each batch in order to reach various concentrations from 0% to 10%. The ground beef samples were stored at refrigerator and ambient temperatures. The raw meatball samples were only stored at room temperature. All samples were analyzed in order to determine the microbial counts at the 2(nd), 6(th), 12(th), and 24(th) hours of storage. Garlic addition decreased the microbial growth in some ground beef samples kept either at room temperature or in the refrigerator. However, microbial growth increased in some ground beef samples kept in similar conditions. The difference was found in samples kept in the refrigerator for 24 hours in terms of total aerobic mesophilic bacteria and coliform bacteria when garlic used at 10%. The effects of garlic on the microbial growth of both coliforms and Staphylococcus/Micrococcus in the samples kept at room temperature were increased. The yeast and mold counts in ground beef samples kept in any condition were not affected by garlic addition. However, the addition of garlic to the raw meatball mix decreased the microbial count, in terms of total aerobic mesophilic bacteria and yeast and mold counts, when the garlic was added at 5% or 10% (P < .05). The addition of 10% garlic to raw meatball caused a permanent decrease in yeast and mold count, unlike in ground beef. The results of this study indicate that the chopped garlic has a slowing-down effect on microbiological growth in ground meat depending on the garlic concentration, but this effect was not at an expected level even at the highest concentration, because potential antimicrobial agents in chopped garlic were probably insufficiently extracted.

Cone yoghurt is a yoghurt variety produced by adding only pine cones to milk without culture in a limited area of Turkey. The present study was conducted to identify and characterize lactic flora in traditional cone yoghurt. Morphological, cultural, physiological, biochemical, and genotypic characteristics were employed to identify lactic acid bacteria isolates from cones and cone yoghurts. subsp. () and subsp. () were obtained from both cones and yoghurts. Among the isolates, was frequently isolated except for these two bacteria ( and ). The results indicate that the cone yoghurt has a mixed microflora contrary to the yoghurt produced by the addition of a starter culture and, and in cone yoghurt originates from the pine cones.

Bu çalışma piyasada hızlı yemek restaurantlarda satışa sunulan pişmiş haldeki dönerlerin mikrobiyolojik kalitesini ortaya koymak ve pişme sonrası bekleme sırasında mikroorganizma sayısındaki değişimleri incelemek amacıyla gerçekleştirilmiştir. Öncelikli olarak İstanbul'da Avcılar bölgesindeki 20 farklı satış noktasından toplam 30 adet döner örneği toplanmış ve mikrobiyolojik analizleri yapılmıştır. Daha sonra üç ayrı işletmeden alınan dönerler oda sıcaklığında bekletilerek belli aralıklarla mikroorganizma sayılarındaki değişimler incelenmiştir. Ayrıca, tüketime hazır döner örnekleri Staphylococcus aureus ile deneysel olarak kontamine edilerek adı geçen mikroorganizmanın oda sıcaklığında bekletme sırasında gelişimi takip edilmiştir. Piyasadan toplanan örneklerde toplam mezofil aerobik bakteri, Enterobacteriaceae, Esherichia coli sayıları arasında önemli farklar görülmüş; hiçbir örnekte S. aureus ve termofilik anaerob sporlu bakteri varlığına rastlanmamıştır. Pişmiş dönerlerin bekletilmesi sırasında ise ilk 6 saat içinde mikroorganizma sayılarında dikkati çeken bir artış gözlenmemiştir. S. aureus ile kontamine edilen örneklerde ise bekletme sırasında benzer durum gözlenmiş ve adı geçen bakteri sayısında önemli bir artış kaydedilmemiştir. Elde edilen bulgulara göre dönerlerin mikrobiyolojik kalitesinin alındığı yere bağlı olarak farklılık gösterdiği; pişmiş dönerlerin mikrobiyel gelişim için uygun bir ortam oluşturmadığı sonucuna varılmıştır.

Investigation of norovirus (NoV) contamination of food items is important because many outbreaks occur after consumption of contaminated shellfish, vegetables, fruits, and water. The frequency of NoV contamination in food items has not previously been investigated in Turkey. The aim of this study was to investigate the frequency of human NoV genogroups (G) I and II in ready-to-eat tomatoes, parsley, green onion, lettuce, mixed salads, and cracked wheat balls. RNA was extracted with the RNeasy Mini Kit, and a real-time reverse transcription (RT) PCR assay was performed using primers specific for NoV GI and GII. Among the 525 samples analyzed, NoV GII was detected in 1 green onion sample and 1 tomato sample by both SYBR Green and TaqMan real-time RT-PCR assays; no GI virus was detected. The Enterobactericaeae and Escherichia coli levels in the NoV-positive green onion were 6.56 and 1.28 log CFU/g, and those in the tomato were 5.55 and 1.30 log CFU/g, respectively. No significant difference in the bacterial levels was found between the NoV-positive and NoV-negative samples. This study is the first in which NoV GII was found in ready-to-eat food collected from Istanbul, Turkey; thus, these foods may be considered a risk to human health. Epidemiological studies and measures to prevent NoV infection should be considered.