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Acellular assays of oxidative potential (OP) induced by ambient particulate matters (PMs) are of great significance in screening for toxicity in PMs. In this review, several typical OP measurement techniques, including the respiratory tract lining fluid assay (RTLF), ascorbate depletion assay (AA), dithiothreitol assay (DTT), chemiluminescent reductive acridinium triggering (CRAT), dichlorofluorescin assay (DCFH) and electron paramagnetic/spin resonance assay (EPR/ESR) are discussed and their sensitivity to different PMs species composition, PMs size distribution and seasonality is compared. By comparison, the DTT assay tends to be the preferred method providing a more comprehensive measurement with transition metals and quinones accumulated in the fine PMs fraction. Specific transition metals (i.e., Mn, Cu, Fe) and quinones are found to contribute OPDTT directly whereas the redox properties of PMs species may be changed by the interactions between themselves. The selection of the appropriate OP measurement methods and the accurate analysis of the relationship between the methods and PM components is conducive to epidemiological researches which are related with oxidative stress induced by PMs exposure.

BackgroundSodium is critical for many physiological functions in insects. Herbivorous insects should expend considerable energy to compensate for sodium deficiency due to low sodium concentration in most inland plants upon which they feed. However, sodium compensation behaviors such as mud-puddling have been observed in some species but not in others. We expect that there may be other sodium compensation strategies in insects. Here, we select a rarely mud-puddling insect species, the cotton boll worm, Helicoverpa armigera, and determine the effects of dietary sodium on performance and flight, and examine their means of sodium compensation.ResultsWhen freshly hatched H. armigera neonates were cultured on one of three diets differing in sodium contents (diet A, B and C with a high, middle and low sodium concentrations, respectively), the larvae on diet C grew larger, had a higher mortality rate and a shorter development period than those on diet A and B. The larvae previously fed from 1st to 3rd instar on diet C consumed more subsequent diet when they were transferred to diet A or C at 4th instar, comparing to those previously fed on diet A. Moreover, any 4th-instar larvae on diet C consumed a greater amount of food than those on diet A, no matter which diet the larvae had previously ingested from 1st to 3rd instar. Moths from diet A and B flew more rapidly than those from diet C, with similar sugar and lipid utilization rates among the three test groups. When a 5th-instar cannibal from diet A, B or C and a 5th-instar victim from diet A were housed together, many more cannibals from diet C ate their victims. When a victim from diet A, B or C was provided, a cannibal from diet C was more likely to eat the victim from diet A. When newly emerged moths had been exposed to 3% sodium chloride solution for all scotophase period, the average weight increase (proxy for sodium solution intake) for moths from diet A was lower than those from diet B or C.ConclusionSodium-deficient diet resulted in rapid growth and development of H. armigera larvae, decreased larvae survival, and reduced flight speed of H. armigera adults. To compensate for sodium deficiency, H. armigera ingested a large quantity of larval food, increased larval cannibalism incidence and harvested sodium during the adult stage.

Water is a critical resource for all terrestrial insects. The yellow-spined bamboo locust Ceracris kiangsu Tsai (Oedipodidae: Orthoptera), matures, copulates and reproduces in the summer and adults are observed mud-puddling in human urine-contaminated materials on hot days. Besides obtaining NaCl and NH 4 HCO 3 , the locust may also compensate for water deficiency. In a dual-choice test in the laboratory, adults prefer to remain on wet rather than dry filter paper at high temperature (36 • C). The females are more likely to move towards and stay on wet filter paper than males. The adults chew wet filter paper in the laboratory at higher temperature and the females consume more than the males. Moreover, foam plastic containing an aqueous solution of an insecticide, bisultap, results in greater mortality in females than a dry formulation, both in the laboratory and the field. The water content of the frass from males is significantly lower than that from females and may partially explain the stronger hygropreference behaviour in females. Other possible reasons for sexually different hygropreference are also discussed. The removal of the adult antennae substantially reduces discrimination between wet and dry filter paper and mortality resulting from wet bisultap formulations in the laboratory. Hence, antennae mediate, at least partially, hygropreference behaviour.