A synthetic scheme for preparation of (Gly-Pro)n, (Pro-Gly)n (n = 2, 3), and (Pro-Gly-Pro)n (n = 1, 2) peptides was elaborated. The effect of the synthesized peptides and the Gly-Pro and Pro-Gly dipeptides on survival of cultured cells of PC12 rat pheochromocytoma was studied under the conditions of oxidative stress induced by brief incubation of the cells with hydrogen peroxide. Peptides of the general formula (Gly-Pro)n and the Pro-Gly-Pro peptide at a concentration of 0.2-100 microM were shown to decrease the number of damaged cells. The Gly-Pro peptide was the most active and decreased the number of damaged cells by 49% on average at a concentration of 100 microM.
The article presents the results of research on the biological properties of the bacteriophage Phagum B. c. 11 UGSHA. Bioinformatic sequence data of Bacillus coagulans phage Phagum B. c. 11 UGSHA: length: 42609 bp, GC content: 37,1 %, molecular weight: 27 014 203,97 Da, the molarity of 1 μg/μl: 0.04 μm, the number of molecules in 1 g: 2.23 x 1010, And 260 of 1 μg/mql after 100-fold dilution: 0,259. Experimentally technological parameters of cultivation system phage/host were selected(0.2 ml of bacteriophage to 0.2 ml of the indicator culture B. coagulans), passage time-6 hours at the cultivation temperature- 35±20С. It is recommended to use Millipore membrane filters of 0.22 μm GV for cleaning Phagum B. c. 11 UGSHA. It was determined that the lytic activity by Appelman was 10-9, by Grazia the indicator was4,0+0,1×1010 ((BFU / ml); Phagum B. c. 11 UGSHA had strict specificity in relation to B. coagulans strains; morphology of plaque-forming units (rounded shape with a transparent center, zones of incomplete lysis, diameter 1-4 mm, secondary growth was not observed). It is empirically established that Phagum B. c. 11 UGSHA did not lose its lytic activity after 3 months when stored at a temperature of 2-40С, and after 12 months the indicator decreased to 108. The studied biological properties of bacteriophage Phagum B. c. 11 UGSHA isolated from fresh tomatoes with signs of spoiling, specific for 46 out of 50 bacterial strains of Bacillus coagulans, allow us to recommend Phagum B. c. 11 UGSHA for the production of phage biopreparations used not only in the laboratory for the indication and identification of Bacillus coagulans bacteria specific to it, but also for decontamination of food raw materials and food products, prevention of food poisoning, since the data of our genetic and proteomic mapping allow us to conclude that the Phagum B. c. 11 UGSHA bacteriophage does not contain pathogenicity locuses and their homologues.