the chelating ability of aqueous leaf extract of Aerva lanata was assessed in vitro. the aqueous leaf extract showed a dose dependent decrease in chelating ability using FeCl 2. the highest chelating ability of aqueous leaf extract was observed at 2. 10-5 g/ml (100 ± 0.00). the antioxidant activity of the aqueous leaf extract ranged from 42.13% to 88.66%. At 2. 10-5 g/ml concentration, a strong positive significant correlation was observed between chelating ability and total phenolics concentration (r = 0.94; P = 0.001). the chelating ability of aqueous leaf extract (2. 10-5 g/ml) showed a high positive significant correlation with antioxidant activity (r = 0.78; P = 0.001). Aqueous leaf extract removed the chromium from tannery effluent by 43 mg/g. Allium cepa toxicity test was performed on tannery effluent treated with aqueous leaf extract that increased the root length of onion.
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