Aqueous state laccase thermostabilization using carbohydrate polymers: Effect on toxicity assessment of azo dye

Poonkuzhali, K.; Sathishkumar, Palanivel; Boopathy, Rathanam; Palvannan, Thayumanavan

doi:10.1016/j.carbpol.2011.02.031

Cited by 16 publications

(16 citation statements)

References 31 publications

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“…Probably, DMP promote lesser conformational changes at high temperature then ABTS and catechol. However, the reason for these sudden variations is still unclear (Poonkuzhali, Sathishkumar, Boopathy, & Palvannan, 2011). The activation energy (E a ) was obtained by nonlinear regression of the kinetic constants from the slope of the straight-line plot produced by the Arrhenius equation (Eq(1)) between 20 and 50 • C. The activation energies of ABTS and catechol were 57 and 48 kJ mol −1 , respectively.…”

Section: Laccase Characterizationmentioning

confidence: 99%

Laccase immobilization on bacterial nanocellulose membranes: Antimicrobial, kinetic and stability properties

Sampaio

Padrão

Faria

et al. 2016

Carbohydrate Polymers

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This work studied the physical immobilization of a commercial laccase on bacterial nanocellulose (BNC) aiming to identify the laccase antibacterial properties suitable for wound dressings. Physico-chemical analysis demonstrates that the BNC structure is manly formed by pure crystalline Iα cellulose. The pH optimum and activation energy of free laccase depends on the substrate employed corresponding to pH 6, 7, 3 and 57, 22, 48kJmol(-1) for 2,6-dimethylphenol (DMP), catechol and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), respectively. The Michaelis-Menten constant (Km) value for the immobilized laccase (0.77mM) was found to be almost double of that of the free enzyme (0.42mM). However, the specific activities of immobilized and free laccase are similar suggesting that the cage-like structure of BNC allows entrapped laccase to maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase in Gram-positive (92%) and Gram-negative (26%) bacteria and cytotoxicity acceptable for wound dressing applications.

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Section: Laccase Characterizationmentioning

confidence: 99%

Laccase immobilization on bacterial nanocellulose membranes: Antimicrobial, kinetic and stability properties

Sampaio

Padrão

Faria

et al. 2016

Carbohydrate Polymers

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“…and Bauhinia candicans Benth with A. cepa and bone marrow cells of Wistar rats showed similar results (Camparoto et al, www.bosaljournals/chemint/ editorci@bosaljournals.com 2002). Cytotoxicities have been assessed by the A. cepa roots tip cell were also found similar to in vitro animal toxicity tests (Poonkuzhali et al, 2011b). In a comparative study, Ma et al (Ma et al, 1995) reported that the A. cepa test was more sensitive and efficient for the detection of environmental pollutants.…”

Section: Advantages Of a Cepa Test Versus Other Biological Assaysmentioning

confidence: 69%

Bioassays based on higher plants as excellent dosimeters for ecotoxicity monitoring: A review

Int¹,

Iqbal²

2019

Preprint

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All sorts of pollution on the planet can be traced back to development of industries and the most important among them is water pollution. Clean technologies, management practices and regular monitoring of effluents could be helpful to minimize the contamination of watersheds. Allium cepa (A. cepa) has been recognized as a promising genetic model to detect the toxicity of industrial wastewater, contaminated soil, river water, nuclear contamination and even for those systems which are considered non toxic. A. cepa is distinguished as a low cost test, easy to handle and sensitive to both in vivo and in vitro models. It offers the detection of damages in genetic material quantitatively and the results can be generalized for other biological and ecological systems. Moreover, the pollutants can be classified on the basis of this test present in industrial effluents and their mechanism of action on genetic material. This review focuses on the studies undertaken to evaluate the toxicity of industrial wastewater, contaminated river and soils.

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“…Allium cepa was exposed for 96 h to chromium effluent treated with aqueous leaf extract. The base of the onion bulbs was grown on plant extract treated chromium effluent (50 mg/L) in the 15 ml boiling test tubes after which the number of roots and its length were measured [16].…”

Section: Methodsmentioning

confidence: 99%

Assessing the chelating ability of Aerva Lanata: Adsorption of chromium from tannery effluent and its toxicity measurement

Poonkuzhali

Manivannan

Palvannan

2013

J. Water Chem. Technol.

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the chelating ability of aqueous leaf extract of Aerva lanata was assessed in vitro. the aqueous leaf extract showed a dose dependent decrease in chelating ability using FeCl 2. the highest chelating ability of aqueous leaf extract was observed at 2. 10-5 g/ml (100 ± 0.00). the antioxidant activity of the aqueous leaf extract ranged from 42.13% to 88.66%. At 2. 10-5 g/ml concentration, a strong positive significant correlation was observed between chelating ability and total phenolics concentration (r = 0.94; P = 0.001). the chelating ability of aqueous leaf extract (2. 10-5 g/ml) showed a high positive significant correlation with antioxidant activity (r = 0.78; P = 0.001). Aqueous leaf extract removed the chromium from tannery effluent by 43 mg/g. Allium cepa toxicity test was performed on tannery effluent treated with aqueous leaf extract that increased the root length of onion.

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Aqueous state laccase thermostabilization using carbohydrate polymers: Effect on toxicity assessment of azo dye

Cited by 16 publications

References 31 publications

Laccase immobilization on bacterial nanocellulose membranes: Antimicrobial, kinetic and stability properties

Laccase immobilization on bacterial nanocellulose membranes: Antimicrobial, kinetic and stability properties

Bioassays based on higher plants as excellent dosimeters for ecotoxicity monitoring: A review

Assessing the chelating ability of Aerva Lanata: Adsorption of chromium from tannery effluent and its toxicity measurement

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