Thermostabilization of laccase by polysaccharide additives: Enhancement using central composite design of RSM

Poonkuzhali, K.; Palvannan, Thayumanavan

doi:10.1016/j.carbpol.2011.05.028

Cited by 18 publications

(23 citation statements)

References 25 publications

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“…For this investigation, a 2 3 fractional factorial CCD for three independent variables consisting of eight factorial points, six axial points, and six replicates at the center points leading to a total number of 20 experiments was used in this study. The number of experimental runs was calculated from the following equation :

N = 2^{k} + 2 k + x_{0}

where N is the number of experiments required, k the number of variables, and x 0 is the number of central points. The range of variables investigated is presented in Tab.…”

Section: Methodsmentioning

confidence: 99%

“…The effects of uncontrolled factors were minimized by randomizing the experimental sequence . The three responses were received at 60°C for 24 h. Second degree polynomials, which include all interaction terms, were used to calculate the predicted response by the following equation:

Y = b_{0} + {true \sum}_{i = 1}^{n} b_{i} x_{i} + {[true \sum_{i = 1}^{n} b_{ii} x_{i}]}^{2} + {true \sum}_{i = 1}^{n - 1} {true \sum}_{j = i + 1}^{n} b_{ij} x_{i} x_{j}

where Y is the predicted response, b 0 the constant coefficient, b i the linear coefficients, b ij the interaction coefficients, b ii the quadratic coefficients, and x i , x j are the coded values of variables of the HRP stabilization . Statistical analysis of the model was carried out in order to evaluate the analysis of variance (ANOVA) .…”

Section: Methodsmentioning

confidence: 99%

“…The most popular one is CCD method. Simplicity and accuracy of the CCD made applicability of this tool to optimize the selected response with fitting a quadratic surface [13,14,[24][25][26][27][28]. It optimizes the successful parameters more effectively with a minimum number of experiments and helps to analyze the interaction between the parameters [24][25][26][27][28].…”

Section: Central Composite Design (Ccd)mentioning

confidence: 99%

“…Several studies have been widely reported on enzyme stabilization in aqueous and non-aqueous media in the presence of carbohydrate additives [3,4,[12][13][14][15]. The stabilization of enzymes using carbohydrate additives is a customary practice in enzyme technology to improve enzyme resistance towards denaturation condition [1,4,16].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Efficiency of Carbohydrate Additives on the Stability of Horseradish Peroxidase (HRP): HRP‐Catalyzed Removal of Phenol and Malachite Green Decolorization from Wastewater

Kalaiarasan

Palvannan

2014

CLEAN Soil Air Water

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The present study was undertaken to develop a cost effective stabilized horseradish peroxidase (HRP) for removal of phenol and decolorization of malachite green from synthetic wastewater. As revealed by enzyme stabilization assays, guar gum and galactose improved the stability of HRP successfully in solution state as compared as other additives such as pectin, carrageenan, or starch, respectively. In order to enhance the stability of HRP, combination of galactose and guar gum were effectively optimized together along with pH by constructing an appropriate response surface methodology (RSM). The RSM results suggest 18.25% of galactose and 0.35% of guar gum for maximum stabilization of HRP at 60°C, pH 7.4. In this study, optimization of removal of phenol and decolorization of malachite green using stabilized HRP was successfully carried out. Various process parameters such as aqueous phase of pH (7.4), concentration of enzyme (1.8 U/mL), phenol concentration (0.1 mM), and reaction time of 180 min were optimized effectively to achieve maximum removal of phenol from synthetic waste water. Dye decolorization studies indicated that stabilized HRP (1.2 U/mL) required lower concentration than the native enzyme (2 U/mL) to obtain similar decolorization efficiency. In addition, stabilized enzyme successfully decolorized the dye at 60°C, pH 7.2.

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N = 2^{k} + 2 k + x_{0}

where N is the number of experiments required, k the number of variables, and x 0 is the number of central points. The range of variables investigated is presented in Tab.…”

Section: Methodsmentioning

confidence: 99%

Y = b_{0} + {true \sum}_{i = 1}^{n} b_{i} x_{i} + {[true \sum_{i = 1}^{n} b_{ii} x_{i}]}^{2} + {true \sum}_{i = 1}^{n - 1} {true \sum}_{j = i + 1}^{n} b_{ij} x_{i} x_{j}

Section: Methodsmentioning

confidence: 99%

Section: Central Composite Design (Ccd)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efficiency of Carbohydrate Additives on the Stability of Horseradish Peroxidase (HRP): HRP‐Catalyzed Removal of Phenol and Malachite Green Decolorization from Wastewater

Kalaiarasan

Palvannan

2014

CLEAN Soil Air Water

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“…Therefore the statistical approach played an important role in optimizing the components for the enhancement of flux. 50 The detailed calculation is described in the ESI. †…”

Section: Selection Of Penetration Enhancersmentioning

confidence: 99%

Accentuated transdermal application of glucosamine sulphate attenuates experimental osteoarthritis induced by monosodium iodoacetate

et al. 2016

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Osteoarthritis is a chronic degenerative joint disease causing pain and disability. Glucosamine sulphate is a well known oral supplement for its treatment. The present pioneering study provides an overview of the accentuated transdermal delivery of glucosamine sulphate through the optimized gel formulation with guar gum and sodium carboxymethyl cellulose (Na-CMC). Response surface methodology based on the three-level three-factor central composite design provided the optimum concentration of guar gum, Na-CMC and glycerol for a maximum flux. The transdermal characterization, ex vivo permeation study and in vivo study were performed with optimized gel formulation. The factorial design predicted the optimum values of guar gum, Na-CMC and glycerol which were 418.53 mg, 444.97 mg and 2322.4 mg respectively for 25 g of the gel. This optimized gel demonstrated the maximum flux,i.e., 1047.46 mg cm À2 h À1 . The optimized gel showed satisfactory results with respect to drug uniformity, pH, stability, rheological properties, zeta potential, drug-excipient compatibility and skin irritation.The release of the drug from the optimized transdermal gel followed the controlled first order Fickian (non-steady) release pattern. The in vivo study was carried out in a rat model of osteoarthritis induced by monosodium iodoacetate damaging the tibial plateau. In this study the optimized formulation effectively reduced the symptoms like reduction in swelling of the knee joint, gross changes in digitized radio images and morphological and histopathological alterations. Additionally the changes in the release pattern of the proinflammatory cytokine tumor necrosis factor-a illustrated the efficacy of the transdermal gel for the treatment of experimental osteoarthritis. Thus the optimized gel was found to be a unique potential vehicle for transdermal application of glucosamine sulphate which effectively attenuates the experimental osteoarthritis.

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Synthesis and characterization of chemical engineered PLGA nanosphere: Triggering mechanism of Catechol-O-methyltransferase inhibition on in vivo neurodegeneration

Poonkuzhali

Seenivasagan

Prabhakaran

et al. 2023

Bioorganic Chemistry

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Thermostabilization of laccase by polysaccharide additives: Enhancement using central composite design of RSM

Cited by 18 publications

References 25 publications

Efficiency of Carbohydrate Additives on the Stability of Horseradish Peroxidase (HRP): HRP‐Catalyzed Removal of Phenol and Malachite Green Decolorization from Wastewater

Efficiency of Carbohydrate Additives on the Stability of Horseradish Peroxidase (HRP): HRP‐Catalyzed Removal of Phenol and Malachite Green Decolorization from Wastewater

Accentuated transdermal application of glucosamine sulphate attenuates experimental osteoarthritis induced by monosodium iodoacetate

Synthesis and characterization of chemical engineered PLGA nanosphere: Triggering mechanism of Catechol-O-methyltransferase inhibition on in vivo neurodegeneration

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