Turnip yellows virus (TuYV) is responsible for a recognizable loss of yield in European winter oilseed rape cultivation. To map genes involved in TuYV resistance, a double haploid population was established by crossing a resynthesized rapeseed line (R54) as donor for TuYV resistance with an elite rapeseed line (‘Express’). Resistance was determined with 10 plants per line by double antibody sandwich‐enzyme‐linked immunosorbent assay. After screening 17 primer combinations (Pstl/Msel and EcoRI/Msel), 143 amplified fragment length polymorphism markers were mapped to 20 linkage groups representing 15 chromosomes of the rapeseed genome. Quantitative trait loci (QTL) were mapped using the composite interval mapping approach. As a result, one major quantitative trait locus was found on linkage group MS17, explaining up to 50% of the phenotypic variation. Because no other factors displaying a significant effect on the expression of resistance could be identified, a simple mode of inheritance for TuYV resistance is suggested, thus enabling marker‐assisted selection during rapeseed breeding.
Using specific primers for the 5'-ends of the RNA of turnip yellows luteovirus (TuYV) and beet mild yellowing luteovirus (BMW) corresponding genomic regions of several isolates were RT-PCR amplified, cloned and sequenced. Comparison of the sequence data support the point of view that both viruses can not be isolates of one and the same virus as they share nearly no common sequence motifs. While the sequences of BMW isolates are rather conserved the sequences of different TuYV isolates revealed a high diversity.KEY WORDS: Beet western yellows virus, turnip yellows virus, beet mild yellowing virus, isolates, monoclonal antibodies, RT-PCR, sequences VERGLEICH DER 5'-TERMINALEN NUKLEOTIDSEQUENZEN VON LUTEOVIREN DES RAPSES UND DER ZUCKERRÜBE Mit Hilfe spezifischer Primer wurden die korrespondierenden 5'-Regionen der RNA des turnip yellows luteovirus (TuYV) und des beet mild yellowing luteovirus (BMYV) verschiedener Isolate mittels PCR amplifiziert, kloniert und sequenziert. Die Analyse der Sequenzdaten unterstützt den Strandpunkt, daß beide Viren nicht Isolate eines Virus sein können, da sie in dieser Region keinerlei gemeinsame Sequenzmotive aufweisen. Während die Sequenzen der BMYV Isolate relativ konserviert sind, weisen die der TuYV Isolate eine hohe Diversität auf.
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