Comparison of the 5'‐end nucleotide sequences of luteoviruses from oilseed rape and sugar beet

Abstract

Using specific primers for the 5'-ends of the RNA of turnip yellows luteovirus (TuYV) and beet mild yellowing luteovirus (BMW) corresponding genomic regions of several isolates were RT-PCR amplified, cloned and sequenced. Comparison of the sequence data support the point of view that both viruses can not be isolates of one and the same virus as they share nearly no common sequence motifs. While the sequences of BMW isolates are rather conserved the sequences of different TuYV isolates revealed a high diversity.KEY WORDS: Beet western yellows virus, turnip yellows virus, beet mild yellowing virus, isolates, monoclonal antibodies, RT-PCR, sequences VERGLEICH DER 5'-TERMINALEN NUKLEOTIDSEQUENZEN VON LUTEOVIREN DES RAPSES UND DER ZUCKERRÜBE Mit Hilfe spezifischer Primer wurden die korrespondierenden 5'-Regionen der RNA des turnip yellows luteovirus (TuYV) und des beet mild yellowing luteovirus (BMYV) verschiedener Isolate mittels PCR amplifiziert, kloniert und sequenziert. Die Analyse der Sequenzdaten unterstützt den Strandpunkt, daß beide Viren nicht Isolate eines Virus sein können, da sie in dieser Region keinerlei gemeinsame Sequenzmotive aufweisen. Während die Sequenzen der BMYV Isolate relativ konserviert sind, weisen die der TuYV Isolate eine hohe Diversität auf.

Distribution and properties of geographically distinct isolates of sugar beet yellowing viruses

“…The assignment of the ORF0 nucleotide sequences for these isolates placed them into groups relating to host specificity. This was similar to the findings of Schubert et al . (1998) and Hauser et al .…”

“…The assignment of the ORF0 nucleotide sequences for these isolates placed them into groups relating to host specificity. This was similar to the findings of Schubert et al (1998) andHauser et al (2000), but differed from those of de Miranda et al (1995), where isolates that infected beet or brassicas were placed together. One isolate from the USA, USA9, was grouped with the European and Chilean isolates; the second, USA17, was grouped alone.…”

Production of a full-length infectious GFP-tagged cDNA clone of Beet mild yellowing virus for the study of plant–polerovirus interactions

“…BWYV) are all closely related within the 3¢ end of their genomes [3,4,20,21]. In contrast, there is little sequence identity within the 5¢ region, particularly ORF0.…”

The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia

“…Total RNAs were extracted by the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) from 100 mg of leaf tissue of six selected samples originating from different localities. RT-PCR was performed using the One-Step RT-PCR Kit (Qiagen) with primers TuYVorf0F and TuYVorf0R amplifying the P0 gene, the most useful for delineation of Poleroviruses (Schubert et al, 1998). The RT-PCR reaction mixture included 400 μM of each of the four dNTPs, 1 μl of RT-PCR enzyme mix, 0.6 μM of each primer, and 1 μl of extracted RNA in a final volume of 25 μl.…”