Nachweis isometrischer Partikeln im Phloemgewebe von Fragaria vesca L. var. semperflorens nach Infektion mit dem Blattscheckungs-Virus der Erdbeere (strawberry mottle virus)

Leistner, Hans-Ulrich; Graichen, K.

doi:10.1080/03235408609440496

Archives Of Phytopathology And Plant Protection

1986

DOI: 10.1080/03235408609440496

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Nachweis isometrischer Partikeln im Phloemgewebe von Fragaria vesca L. var. semperflorens nach Infektion mit dem Blattscheckungs-Virus der Erdbeere (strawberry mottle virus)

Hans-Ulrich Leistner

K. Graichen

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1991

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“…The electron dense portions of these particles were 17 to 22 nm in diameter. Similar results were obtained by Leistner and Graichen (1986) and by Polfik and Bezpalcovfi (1987), who found these particles in infected C. quinoa leaves, too. Adams and Barbara (1986) tried to purify SMA from infected C. quinoa plants, but had no success.…”

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confidence: 88%

Attempts at Multiplication, Purification, Electron Microscopy, and Characterisation of Three Isolates of the Strawberry Mottle Agent

Polák¹,

Meer

Huttinga

1991

Biol. plant.

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Three isolates of strawberry mottle agent (SMA) from strawberry plants were regularly maintained and multiplied by mechanical inoculation on Chenopodium quinoa plants showing mosaic and mottle symptoms. The use of 5 mM borate buffer pH 8.6 or tap water pH 6.6-7.9 with 4 % (m/v) charcoal for homogenization resulted usually in 100 % infection. The total of 2090 plants were infected from 2264 inoculated ones under the same conditions. The infectivity of SMA isolates in crude sap of C. quinoa was retained from 48 h to 72 h at 20 ~ The dilution end points of SMA isolates were 10 -3 while the inactivation temperatures were between 50 and 55 ~ The infectivity of SMA isolates in frozen leaves ofC. quinoa was detected still after six months.Purification procedure of SMA is based on using low molar 25 mM borate buffer pH 8.3 with cysteine hydrochloride, DIECA and Tween 20 for homogenization of infected C. quinoa leaves, polyethyleneglycol precipitation, clarification with oetanol, low and high speed centrifugation and sucrose density-gradient centrifugation. Partially purified preparations are highly infectious, causing mosaic, mottling and tip necrosis of C. quinoa plants. The agent could not be completely separated from host proteins and it could not be concentrated to a high extent. Isometric virus-like particles 14-16 nm were observed in partially purified preparations.

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supporting

confidence: 88%

Attempts at Multiplication, Purification, Electron Microscopy, and Characterisation of Three Isolates of the Strawberry Mottle Agent

Polák¹,

Meer

Huttinga

1991

Biol. plant.

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Nachweis isometrischer Partikeln im Phloemgewebe von Fragaria vesca L. var. semperflorens nach Infektion mit dem Blattscheckungs-Virus der Erdbeere (strawberry mottle virus)

Cited by 1 publication

References 2 publications

Attempts at Multiplication, Purification, Electron Microscopy, and Characterisation of Three Isolates of the Strawberry Mottle Agent

Attempts at Multiplication, Purification, Electron Microscopy, and Characterisation of Three Isolates of the Strawberry Mottle Agent

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