The distinctive cellular and mitochondrial dysfunctions of two human lung cancer cell lines (H460 and HCC1588) from two human lung normal cell lines (MRC5 and L132) have been studied by dielectric barrier discharge (DBD) plasma treatment. This cytotoxicity is exposure time-dependent, which is strongly mediated by the large amount of H2O2 and NOx in culture media generated by DBD nonthermal plasma. It is found that the cell number of lung cancer cells has been reduced more than that of the lung normal cells. The mitochondrial vulnerability to reactive species in H460 may induce distinctively selective responses. Differential mitochondrial membrane potential decrease, mitochondrial enzymatic dysfunction, and mitochondrial morphological alteration are exhibited in two cell lines. These results suggest the nonthermal plasma treatment as an efficacious modality in lung cancer therapy.

Atmospheric pressure non‐equilibrium plasmas are efficacious in killing both prokaryotic and eukaryotic cells. While the mechanism of plasma induced cell death has been thoroughly studied in prokaryotes, detailed investigation of plasma mediated eukaryotic cell death is still pending. When plasma is generated, four major components that interact with cells are produced: electric fields, radiation, charged particles, and neutral gas species. The goal of this study was to determine which of the plasma components are responsible for plasma‐induced cell death by isolating and removing each from treatment. The C3H10T1/2 murine mesenchyme stem cell line was treated in six well plates, stained with Propidium Iodide to determine viability, and analyzed by image cytometry. Our results show that plasma‐generated charges and reactive oxygen species are the primary contributors to cell death.

In this manuscript we discuss a chemical kinetic model of blood coagulation by treatment with nonequilibrium atmospheric pressure dielectric barrier discharge. We then add kinetic data into a computational fluid dynamics model of blood flow treated by plasma and show that we are able to induce hemostasis. We then present initial experimental validation of these computational models using in vitro bovine blood samples.

Plasma Medicine is the newest and rapidly expanding area of engineering medicine and bioengineering focused on direct applications of plasma for treatment of different diseases, blood coagulation control, wound management, and wound healing, as well as improving patient care through sterilization, medical implants, biomaterial engineering, and tissue engineering. The number of medical engineering professionals, researchers, upper undergraduate, and graduate university students involved today in plasma medicine is already large and growing; there is also an exponentially growing number of publications in this new field. All these students and professionals need the ability to find these publications to aid them in getting started and to advance in their research in plasma medicine. This determines the main purpose of this review, focused on, first of all, summarizing the major directions of fundamental plasma medicine and providing an extensive guide to specific diseases with current plasma-bioengineering solutions, as well as providing a relevant, up-to-date bibliography.

Recent research in plasma applications in bioengineering and plasma medicine gives attention to plasma treatment of various liquids, especially water, for the growing number of medical and biological applications. In this paper we present a forward-vortex nonequilibrium atmospheric pressure plasma system for generation of nitrogen and oxygen reactive species as well as reduced pH in flowing water. We discuss the dependence of pH and nitrate produced in water on both the carrier gas and the gas feed rate.