Nonthermal Plasma-Assisted Trauma Management: Hemostasis of Noncompressible Profuse Hemorrhage

Amritkar, Atharva; Cunningham, B. A.; Hawkins, Bradly; Batalla, Brennan; Moore, Dave; Thompson, Eric M.; Rossett, Matt; Gupta, Rittick; Han, Justine; Johnson, Justin

doi:10.1615/plasmamed.2014012024

Cited by 8 publications

(3 citation statements)

References 12 publications

(17 reference statements)

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“…Great efforts are currently made extending the range of plasma applications with different devices. This includes blood coagulation [Amritkar et al, 2013;Aleinik et al, 2017;Miyamoto et al, 2017], ophthalmology [Rosani et al, 2015;Ye et al, 2015;Recek et al, 2016], oncology [Ye et al, 2015;Mirpour et al, 2016;Binenbaum et al, 2017], and various diseases in dermatology [Daeschlein et al, 2010[Daeschlein et al, , 2011Klebes et al, 2014]. Addressing not only the efficacy but also safety of different plasma sources or their optimum operational settings, genotoxic testing is indispensable.…”

Section: Introductionmentioning

confidence: 99%

High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma

Bekeschus

Schmidt

Krämer

et al. 2018

Environ and Mol Mutagen

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Promising cold physical plasma sources have been developed in the field of plasma medicine. An important prerequisite to their clinical use is lack of genotoxic effects in cells. During optimization of one or even different plasma sources for a specific application, large numbers of samples need to be analyzed. There are soft and easy-to-assess markers for genotoxic stress such as phosphorylation of histone H2AX (γH2AX) but only few tests are accredited by the OECD with regard to mutagenicity detection. The micronucleus (MN) assay is among them but often requires manual counting of many thousands of cells per sample under the microscope. A high-throughput MN assay is presented using image flow cytometry and image analysis software. A human lymphocyte cell line was treated with plasma generated with ten different feed gas conditions corresponding to distinct reactive species patterns that were investigated for their genotoxic potential. Several millions of cells were automatically analyzed by a MN quantification strategy outlined in detail in this work. Our data demonstrates the absence of newly formed MN in any feed gas condition using the atmospheric pressure plasma jet kINPen. As positive control, ionizing radiation gave a significant 5-fold increase in micronucleus frequency. Thus, this assay is suitable to assess the genotoxic potential in large sample sets of cells exposed chemical or physical agents including plasmas in an efficient, reliable, and semiautomated manner. Environ. Mol. Mutagen. 59:268-277, 2018. © 2018 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 99%

High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma

Bekeschus

Schmidt

Krämer

et al. 2018

Environ and Mol Mutagen

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“…These stages can be influenced by CAP and thus make a final contribution to improved wound healing. Several studies have suggested CAP technology to be a useful tool for promoting hemostasis [ 28 , 29 ]. Furthermore, improved proliferation and remodeling after CAP treatment were observed [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma Promotes the Immunoreactivity of Granulocytes In Vitro

et al. 2021

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Cold atmospheric plasma (CAP) reduces bacteria and interacts with tissues and cells, thus improving wound healing. The CAP-related induction of neutrophils was recently described in stained sections of wound tissue in mice. Consequently, this study aimed to examine the functionality of human polymorphonuclear cells (PMN)/granulocytes through either a plasma-treated solution (PTS) or the direct CAP treatment with different plasma modes and treatment durations. PTS analysis yielded mode-dependent differences in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) after CAP treatment. Live-cell imaging did not show any chemo-attractive or NETosis-inducing effect on PMNs treated with PTS. The time to maximum ROS production (TmaxROS) in PMNs was reduced by PTS and direct CAP treatment. PMNs directly treated with CAP showed an altered cell migration dependent on the treatment duration as well as decreased TmaxROS without inducing apoptosis. Additionally, flow cytometry showed enhanced integrin and selectin expression, as a marker of activation, on PMN surfaces. In conclusion, the modification of PMN immunoreactivity may be a main supporting mechanism for CAP-induced improvement in wound healing.

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“…Cold atmospheric (physical) plasma (CAP) has been suggested for blood coagulation already a decade ago [7] and many times thereafter [8][9][10][11]. Yet, it was only until recently that this approach was verified in surgically and clinically relevant animal models using liver incisions.…”

Section: Introductionmentioning

confidence: 99%

The feed gas composition determines the degree of physical plasma-induced platelet activation for blood coagulation

Bekeschus

Brüggemeier

Hackbarth

et al. 2018

Plasma Sources Sci. Technol.

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Cold atmospheric (physical) plasma has long been suggested to be a useful tool for blood coagulation. However, the clinical applicability of this approach has not been addressed sufficiently. We have previously demonstrated the ability of a clinically accepted atmospheric pressure argon plasma jet (kINPen ® MED) to coagulate liver incisions in mice with similar performance compared to the gold standard electrocauterization. We could show that plasmamediated blood coagulation was dependent on platelet activation. In the present work, we extended on this by investigating kINPen ® -mediated platelet activation in anticoagulated human donor blood ex vivo. With focus on establishing high-throughput, multi-parametric platelet activation assays and performing argon feed gas parameter studies we achieved the following results: (i) plasma activated platelets in heparinized but not in EDTA-anticoagulated blood; (ii) plasma decreased total platelet counts but increased numbers of microparticles; (iii) plasma elevated the expression of several surface activation markers on platelets (CD62P, CD63, CD69, and CD41/61); (iv) in platelet activation, wet and dry argon plasma outperformed feed gas admixtures with oxygen and/or nitrogen; (v) plasma-mediated platelet activation was accompanied by platelet aggregation. Platelet aggregation is a necessary requirement for blood clot formation. These findings are important to further elucidate molecular details and clinical feasibility of cold physical plasma-mediated blood coagulation.

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Nonthermal Plasma-Assisted Trauma Management: Hemostasis of Noncompressible Profuse Hemorrhage

Cited by 8 publications

References 12 publications

High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma

High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma

Cold Atmospheric Plasma Promotes the Immunoreactivity of Granulocytes In Vitro

The feed gas composition determines the degree of physical plasma-induced platelet activation for blood coagulation

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