Cold Atmospheric Plasma Promotes the Immunoreactivity of Granulocytes In Vitro

Abstract

Cold atmospheric plasma (CAP) reduces bacteria and interacts with tissues and cells, thus improving wound healing. The CAP-related induction of neutrophils was recently described in stained sections of wound tissue in mice. Consequently, this study aimed to examine the functionality of human polymorphonuclear cells (PMN)/granulocytes through either a plasma-treated solution (PTS) or the direct CAP treatment with different plasma modes and treatment durations. PTS analysis yielded mode-dependent differences in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) after CAP treatment. Live-cell imaging did not show any chemo-attractive or NETosis-inducing effect on PMNs treated with PTS. The time to maximum ROS production (TmaxROS) in PMNs was reduced by PTS and direct CAP treatment. PMNs directly treated with CAP showed an altered cell migration dependent on the treatment duration as well as decreased TmaxROS without inducing apoptosis. Additionally, flow cytometry showed enhanced integrin and selectin expression, as a marker of activation, on PMN surfaces. In conclusion, the modification of PMN immunoreactivity may be a main supporting mechanism for CAP-induced improvement in wound healing.

Enhancement of the Plant Grafting Technique with Dielectric Barrier Discharge Cold Atmospheric Plasma and Plasma-Treated Solution

“…Deferent plasma generation methods and setups have been presented and have also shown that plasma-treated solutions can be used along with CAP sources for “indirect” treatment [ 46 , 47 ]. The influence of plasma treatment on DNA damage, gene expression, enzymatic activity, morphological and chemical changes, germination, and resistance to stress is under research [ 48 , 49 , 50 ]. Many papers have reported promising results in this wide variety of applications [ 51 , 52 ].…”

Paralytic Impact of Centrifugation on Human Neutrophils

“…ROS-production was detected by oxidation of non-fluorescent Dihydrorhodamine (DHR) 123 (10 µM, Thermo Fisher Scientific) to fluorescent Rhodamine 123. This process has already been described in a more detailed way in previous publications of our department [ 19 , 20 ].…”

Current State of Cold Atmospheric Plasma and Cancer‐Immunity Cycle: Therapeutic Relevance and Overcoming Clinical Limitations Using Hydrogels

“…[ 136 ] Like macrophages, neutrophils also show plasticity and can be reprogrammed by the TME. As different CAP treatment regimens could lead to differential responses in neutrophils, [ 137 ] it would be interesting to analyze this in the context of cancer treatment.…”