Telah dilakukan penelitian tentang Uji Toksisitas Akut dan LD50 Ekstrak Etanol Daun Kirinyuh (Euphatorium odoratum Linn) Pada Mencit (Mus musculus.Penelitian ini bertujuan untuk mengetahui nilai Lethal Dosis 50 (LD50) atau dosis dari ekstrak etanol daun kirinyuh yang dapat membunuh 50% mencit (Mus musculus), sehingga dapat memberikan data dasar keamanan dosis dari daun kirinyuh yang dapat digunakan. Dalam penelitian ini menggunakan metode perhitungan Reed dan Muench, yang menggunakan 30 ekor mencit masing-masing 15 ekor betina dan jantan yang dibagi menjadi 5 kelompok perlakuan, masing-masing terdiri atas 6 ekor mencit 3 ekor betina dan 3 ekor jantan. Kelompok I NaCMC 1% sebagai Kontrol , Kelompok II diberi ekstrak etanol daun kirinyuh dengan dosis 10% b/v. Kelompok III diberi ekstrak etanol daun kirinyuh dengan dosis 20% b/v. Kelompok IV diberi ekstrak etanol daun kirinyuh dengan dosis 40% b/v, sedangkan untuk Kelompok V diberikan dosis tertinggi 80% b/v pada mencit. Sediaan uji diberikan secara oral dengan hanya satu kali pemberian pada awal masa penelitian. Gejala toksik yang dominan yaitu parasimpatomimetik (diare dan urinasi). Hasil penelitian yang didapatkan Nilai LD50 yang diperoleh dari hasil pengujian toksisitas akut ekstrak etanol daun kirinyuh (Euphatorium odoratum Linn) yaitu sebesar 14,1416 g/Kg BB atau 28,82 % ekstrak dan termasuk dalam kategori “Toksik Ringan” Kata kunci : Daun Kirinyuh (Euphatorium odoratum Linn), Ekstrak kental, Toksisitas akut, LD50
Leucorrhoea is one of the many disorders of the reproductive organs in women caused by a fungus which is characterized by excessive discharge of fluid in the female organs, causing an unpleasant odor. The fungus Candida albicans is a normal flora found in the female genitalia that causes vaginal discharge. Iler leaves (Coleus scutellariodes L) contain active components, including: flavonoids, tannins, triterpenoids, steroids and volatile oils that have antimicrobial properties. The purpose of this study was to determine the effect of giving female cleansing liquid soap containing slobber leaf extract on the growth of the fungus Candida albicans and to determine the optimal concentration that can inhibit / kill the growth of the fungus Candida albicans. The type of research carried out was a laboratory experiment. , the material used as the active substance is slobber leaves then extracted by maceration using a liquid extract, namely 96% v/v alcohol, the liquid extract obtained was stiffened by evaporation to obtain a thick extract. The active substance of slobber leaf extract is used in the manufacture of liquid soap for feminine hygiene with varying levels of 1%, 2%, and 4%. The feminine cleansing soap that has been made is then tested for the quality of the preparations, including pH and organoleptic testing, while testing its antifungal power against the growth of the fungus Candida albicans using the disk diffusion method (Test Kirby and Bauer). Based on the results of the study, it was shown that female cleansing liquid soap containing the active substance of Iler leaf extract at a concentration of 4% could inhibit/kill the growth of the Candida albicans fungus optimally when compared to concentrations of 1% and 2%, but the effect was still lower than of positive control ( p<0.05).
The flavonoids content of the leaf of Jamblang or Java plum (Egenia cumini Merr.) have the potential to be developed as an anti-microbial agents, especially for Streptococcus pyogenes infections. This research aims to determine the antibacterial activity of Java plum leaf extract (Egenia cumini Merr.) against the growth of Streptococcus pyogenes bacteria. The java plum leaf obtained in the Tamalanrea area of Makassar City was extracted using 96% ethanol by maceration. The dry extract obtained was prepared with concentrations of 2%, 4% and 8% w/v respectively and then tested for anti-bacteria by the agar diffusion method using Nutrient Agar media with a tetracycline comparison. The results showed that the inhibition zone diameter in Java plum Leaf Extract (Egenia cumini Merr.) at a concentration of 2% w/v was 29.0 mm, 4% w/v was 35.0 mm, and 8% w/v was 42.0 mm as well as with Tetracyclines was 78, mm. Therefore, it was concluded that Java plum Leaf Extract (Egenia cumini Merr.) at a concentration of 8% w/v has the greatest antibacterial power (p <0.05), but the effect was smaller compared to Tetracycline (p <0.05).
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