UJI TOKSISITAS AKUT DAN LD50 EKSTRAK ETANOL DAUN KIRINYUH (Euphatorium odoratum Linn) PADA MENCIT (Mus musculus)

Background: Indian almond (Terminalia catappa) is used as an alternative medicine by the community to treat various diseases. However, limited research has been conducted on the subchronic toxicity of Indian almond water extracts on the liver. Present study aimed to determine the subchronic toxic effects of Indian almond leaf water extract on the liver histology of mice and to evaluate the safe dose of Indian almond leaf water extract with various doses (125, 250, 500, 750 and 1000 mg/kg). Methods: In total of 33 male mice were randomly divided into 11 groups, each group consisting of three mice as replication. For 30 days, mice in five treatment groups were orally treated every day with water extracts of green Indian almond leaf while other five groups were given water extracts of brown Indian almond leaves and compare with control group. At the end of day 30, all mice were sacrificed, and hepar was taken out for histological preparations. The histopathological changes in the liver was observed and assessed based on histological damage in the term of degeneration and necrosis. Results: The administration of green and brown Indian almond water extract at doses of 125, 250, 500, 750 and 1000 mg / kg BB showed a toxic effect on the liver of mice. The toxic effect was due to the high doses, causing a cell swelling damage and increased liver necrosis. Conclusion: Indian almond leaf water extract can cause toxic effects on the liver of mice at the doses implemented.

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“…Saponin also causes hemolysis of red blood cells. Both tannins and saponins also induce liver cell damage (Chen et al, 2017;Jumain et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Subchronic Toxicity Test of Indian Almond (Terminalia catappa) Leaves Water Extract on The Liver Histology of Mice (Mus musculus)

Rudy

Puspa

Aryani

et al. 2020

BES

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“…Uji yang dapat digunakan untuk menentukan efek toksisitas dari suatu zat yaitu uji toksisitas akut. Uji toksisitas akut memiliki tujuan akhir untuk menentukan lethal dose 50 (LD50 ) dari suatu zat yang diuji [5] . Uji toksisitas LD50 merupakan nilai potensi toksisitas akut dari suatu obat atau sediaan [6] .…”

Section: Pendahuluanunclassified

Uji Toksisitas Akut Minyak Kemiri (Aleurites moluccana L.) pada Mencit (Mus musculus)

Putri,

Mayasari,

Andriyanto

2024

J. Vet. Biomed.

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Kemiri (Aleurites moluccana L.) merupakan tanaman yang memiliki manfaat dari setiap bagiannya khususnya biji kemiri yang diolah untuk menghasilkan minyak. Penelitian ini bertujuan menghitung tingkat toksisitas minyak kemiri pada mencit dengan menggunakan penentuan nilai lethal dose 50 (LD50). Penelitian ini menggunakan rancangan percobaan acak lengkap. Sebanyak 20 ekor mencit betina dibagi menjadi lima kelompok yaitu kelompok kontrol negatif dan kelompok perlakuan yang diberikan minyak kemiri dosis 5, 10, 15, dan 20 g/kg BB secara peroral. Pengamatan gejala toksisitas pada mencit pascaperlakuan dilakukan selama 14 hari. Parameter yang diamati berupa mortalitas, gejala klinis, dan respon fisiologis mencit. Hasil pada pengamatan dengan parameter mortalitas hewan uji menunjukkan bahwa tidak ada mencit yang mengalami kematian sampai dengan dosis 20 g/kg BB. Parameter respon fisiologis seperti suhu tubuh dan frekuensi nafas berada di rentang normal, sedangkan frekuensi denyut jantung berada di bawah rentang normal. Kesimpulan penelitian ini yaitu minyak kemiri termasuk kedalam kategori praktis tidak toksik.

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“…Penelitian bertujuan untuk mengetahui efektifitas ekstrak buah mengkudu terhadap kematian mencit. (Jumain et al, 2018;Mustapa et al, 2015;Nonci et al, 2014).…”

Section: Pendahuluanunclassified

EFEKTIFITAS EKSTRAK BUAH MENGKUDU (Morinda citrifolia) SEBAGAI PESTISIDA NABATI UNTUK PENGENDALIAN MENCIT (Mus musculus)

Putri¹,

Hajimi²,

Akhmadi³

2022

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Pests often found in homes and harmful to humans are mice (Mus musculus). In addition to most ap and destroyer of building, Mus musculus also acts as a spreader of disease for humans. Controls carried out by the community are making traps or traps, rat poison, and smoking. In addition, there is a natural way, namely using noni fruit. This study aims to determine the effectiveness of noni fruit extract (Morinda citrifolia) as a vegetable pesticide against the control of mice (Mus musculus) and to determine differences in the effectiveness of noni fruit extract (Morinda citrifolia) at each dose variation of 35%, 40%, 45%, 50 %, and 55%. This study uses a quasi-experimental design (quasi-experimental). The number of samples is 30 mice. Toxicity test on mice using the fixed-dose method. The statistical test used is the one-way Anova test. The results showed that there was an effect of the effectiveness of noni fruit extract (Morinda citrifolia) on the mortality of mice (p-value = 0.000 <0.05), and at a concentration of 55% it was effective for killing mice (Mus musculus). However, at a concentration of 55%, it cannot be said to be effective in killing mice (Mus musculus) because it can only kill 20% of mice.

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UJI TOKSISITAS AKUT DAN LD50 EKSTRAK ETANOL DAUN KIRINYUH (Euphatorium odoratum Linn) PADA MENCIT (Mus musculus)

Cited by 14 publications

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Subchronic Toxicity Test of Indian Almond (Terminalia catappa) Leaves Water Extract on The Liver Histology of Mice (Mus musculus)

Subchronic Toxicity Test of Indian Almond (Terminalia catappa) Leaves Water Extract on The Liver Histology of Mice (Mus musculus)

Uji Toksisitas Akut Minyak Kemiri (Aleurites moluccana L.) pada Mencit (Mus musculus)

EFEKTIFITAS EKSTRAK BUAH MENGKUDU (Morinda citrifolia) SEBAGAI PESTISIDA NABATI UNTUK PENGENDALIAN MENCIT (Mus musculus)

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