The typical nitrogen supply recommendation for soybeans is the application of inoculant with no additional required supplementation via fertilization. However, the adoption of no-till farming, the release of high-yielding cultivars and recent studies concerning soybean response to late application of nitrogen have sparked doubts about the possible benefits of nitrogen fertilization. Recent studies using nitrogen fertilization of soybeans show no increase in yield, due to efficient biological fixation (BNF); however, the effects of different application times have not been widely studied. In this context, the aim of this study was to investigate different methods and times of nitrogen fertilization of soybeans. Using the recommended supplements, along with a search of the scientific literature on the same theme, nine treatments were evaluated. These consisted of the application of single and split doses of nitrogen, at 20 kg.ha -1 and 40 kg.ha -1 , during sowing and reproductive stage R4. The nitrogen source used was urea (45% nitrogen). It was applied in adequate environmental conditions, at levels typically found in rain forecasts, and were incorporated into the soil to avoid losses by volatilization. The split application of nitrogen (at sowing + stage R4) provided an increase in yield, reaching a 47% difference in treatment 6 in relation to control, not taking into account supplementation costs. Furthermore, the variables related to plant architecture displayed no significant differences.

Conyza spp. is among the main weeds reported worldwide. Due to its aggressiveness, such as high seed production and dispersion, and the growing reports of biotypes resistant to glyphosate, paraquat, and other herbicides, different control practices are required. Thus, this study aimed to evaluate the efficacy of herbicides with sequential application of glufosinate in soybean pre-sowing for control of Conyza spp. with indicative of resistant to paraquat. The study was carried out in the field, at Assis Chateaubriand and Palotina, state of Paraná, Brazil, in the 2018/19 season. The experiments were conducted in a randomized block design with four replications. The treatments consisted in application of glyphosate, 2,4-D, saflufenacil, glufosinate, saflufenacil/imazethapyr, diclosulam, paraquat/diuron, paraquat and imazethapyr/flumioxazin, at different combinations, in soybean pre-sowing. Control of Conyza spp., crop injury to soybean plants and variables related to agronomic performance (plant height and yield) were evaluated. All treatments were selective for soybean, which showed stronger crop injury in the presence of diclosulam herbicide, but this did not compromise soybean agronomic performance. In general, control levels were high for the treatments used. Except for paraquat treatments, in the area with the highest frequency of Conyza spp. with indicative of resistant to paraquat, and imazethapyr/flumioxazin treatment in both areas. These control results emphasized the importance of glufosinate in this management system and showed promising results for saflufenacil/imazethapyr.

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-Recent studies indicate that glyphosate applied in post-emergence in RR soybean can eventually cause phytotoxic effects. However, there are many questions that need to be clarified in the scientific and technical contexts, involving the issue of RR soybeans regarding the use of glyphosate. This study has assessed the impact of the application of different doses and formulations of glyphosate in the reproductive period of RR soybean (R1 stage). For that purpose, an experiment in the field was conducted in two harvests (2011/12 and 2012/13), in which a 2 x 5 factorial design was used (formulations versus doses) totaling 10 treatments. In these two experiments the variables related to agronomic performance were: phytotoxicity (7, 14, 21 and 28 days after application), plant height, number of pods per plant, yield and weight of 100 grains (end of soy cycle). The results obtained allowed characterizing phytotoxicity and damages to the height and yield in RR soybean, with increasing rates of glyphosate applied in the reproductive period.Keywords: Glycine max, herbicide, transgenic crops, phytotoxicity. ALBRECHT, A.J.P. et al. RESUMO -Resultados

Conyza sumatrensis was reported to be associated with 20 cases of herbicide resistance worldwide, with a recent report of multiple drug resistance to paraquat, glyphosate, and chlorimuron in Brazil. In Paraguay, there were no reports of cases of resistance for this species; however, in 2017, researchers began identifying biotypes with resistance to paraquat, glyphosate, and chlorimuron, which is the focus of the present study. The goal of this study was to investigate the case of multiple resistance of C. sumatrensis to paraquat, glyphosate, and chlorimuron and to monitor the resistant biotypes in the departments of Canindeyú and Alto Paraná. Seeds were collected from sites where plants survived after herbicide application in the 2017/18 and 2018/19 seasons. After screening, biotypes were selected for the construction of dose–response curves. A resistance factor (RF) of 6.79 was observed for 50% control (C50) and 3.92 for 50% growth reduction (GR50) for the application of paraquat. An RF of 12.32 was found for C50 and 4.15 for GR50 for the application of glyphosate. For the application of chlorimuron, an RF of 11.32 was found for C50 and 10.96 for GR50. This confirms the multiple resistance of the C. sumatrensis biotype to paraquat, glyphosate, and chlorimuron. Population monitoring indicated the presence of C. sumatrensis with multiple resistance in departments of Canindeyú and Alto Paraná, Paraguay.

Together, Conyza bonariensis, C. canadensis and C. sumatrensis show 105 reported cases of biotypes resistant to herbicides like glyphosate, paraquat, and acetolactate synthase (ALS) inhibitors. The application of pre-emergent herbicides combined with burndown herbicides is believed to be effective in controlling Conyza spp. during soybean pre-sowing management. The objective of this study was to evaluate the efficacy of sulfentrazone/diuron, imazethapyr/flumioxazin, and diclosulam in mixtures with diquat, paraquat, or glufosinate on the control of Conyza spp. Two field experiments were conducted in a randomized block design with four replicates. Treatments consisted of the application of pre-emergent plus burndown herbicides, besides the weedy control treatment (without application), for a total of 10 treatments. The control of Conyza spp. was evaluated at 7, 14, 21, 28, and 35 d after herbicide application, and symptoms of injury in soybean plants were evaluated at 14, 21, 28, and 35 d after herbicide application. The herbicides sulfentrazone/diuron, imazethapyr/flumioxazin, and diclosulam in combination with burndown herbicides diquat, paraquat, or glufosinate were effective in controlling Conyza spp. in the pre-sowing management of soybean, highlighting good options for pre- and post-emergent herbicide rotations. Mixtures with diclosulam showed a higher potential for injury to soybean plants than sulfentrazone/diuron and imazethapyr/flumioxazin.

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Herbicide mixtures, use of multiple sites of action, and other weed management practices are necessary to avoid cases of biotype resistance. The aim of this study was to evaluate the efficiency of imazapic/imazapyr and other herbicides in mixtures to control Digitaria insularis at burndown before soybean sowing. This field research was conducted in Umuarama, State of Parana (PR), Brazil, in the 2018/19 soybean season. The experiment was conducted in a randomized block experimental design with four replications and 11 treatments composed of the application of glyphosate, clethodim, haloxyfop, imazapic/imazapyr, glufosinate, 2,4-dichlorophenoxyacetic acid (2,4-D), dicamba, triclopyr, and saflufenacil, in mixtures. Weed control was evaluated as well as soybean injury and yield. An analysis of variance and F-test were performed, and the treatment means were compared by the Scott-Knott test. All treatments showed great control over the weed and low crop injury rate while maintaining soybean yield. The application of imazapic/imazapyr in mixtures with other herbicides was effective in controlling glyphosate-resistant D. insularis in burndown before soybean sowing and with sequential application of haloxyfop + glyphosate at V3 stage of soybean. This chemical management was also selective for soybean.