In this study, the chemical characterization and the antioxidant activity of the red (Psidium cattleianum) and yellow (P. cattleianum var. lucidum Hort.) strawberry guava fruits were investigated. This is important for potential application of strawberry guava as functional food. Moreover, it may be the experimental basis for further development and use in food industry.

Besifloxacin (BSF) is a synthetic chiral fluoroquinolone developed for the topical treatment of ophthalmic infections. The present study reports the development and validation of a microbiological assay, applying the cylinder-plate method, for determination of BSF in ophthalmic suspension. To assess this methodology, the development and validation of the method was performed for the quantification of BSF by high performance liquid chromatography (HPLC). The HPLC method showed specificity, linearity in the range of 20-80 µg mL(-1) (r=0.9998), precision, accuracy and robustness. The microbiological method is based on the inhibitory effect of BSF upon the strain of Staphylococcus epidermidis ATCC 12228 used as a test microorganism. The bioassay validation method yielded excellent results and included linearity, precision, accuracy, robustness and selectivity. The assay results were treated statistically by analysis of variance (ANOVA) and were found to be linear (r=0.9974) in the range of 0.5-2.0 µg mL(-1), precise (inter-assay: RSD=0.84), accurate (101.4%), specific and robust. The bioassay and the previously validated high performance liquid chromatographic (HPLC) method were compared using Student's t test, which indicated that there was no statistically significant difference between these two methods. These results confirm that the proposed microbiological method can be used as routine analysis for the quantitative determination of BSF in an ophthalmic suspension. A preliminary stability study during the HPLC validation was performed and demonstrated that BSF is unstable under UV conditions. The photodegradation kinetics of BSF in water showed a first-order reaction for the drug product (ophthalmic suspension) and a second-order reaction for the reference standard (RS) under UVA light. UVA degraded samples of BSF were also studied in order to determine the preliminary in vitro cytotoxicity against mononuclear cells. The results indicated that BSF does not alter the cell membrane and has been considered non-toxic to human mononuclear cells in the experimental conditions tested.

Fruits of Myrcianthes pungens Berg. Legr. (Myrtaceae), known as guabiju, are widely consumed fresh as well as dried, processed into jam, marmalade, and juices. In this study, chemical composition and antichemotactic and antioxidant activities of fruits from a wild type (GB) and 2 genotypes, PL2 and PL1, of guabiju were investigated. Total anthocyanins for the genotypes ranged from 334 to 531 mg/100 g dry weight (dwt). Total flavonoids and polyphenols ranged from 79.8 to 154 mg/100 g and 2438 to 4613 mg/100 g (dwt), respectively. A reversed phase liquid chromatography method with photodiode array detection was used to determine chemical profiles of the main anthocyanins found in the extracts. An HPLC method for the quantification of flavonoids is proposed, providing a simple procedure with rapid sample preparation. All samples contained 5 identical anthocyanidins, distributed differently, with cyanidin as the main compound. Identified flavonoids were quercitrin, hyperoside, and isoquercitrin; their relative amounts varied among the extracts. The antioxidant activity of guabiju methanolic extract was comparable to that of Trolox, and at a test concentration of 0.25 mg/mL, GB and PL2 activities were higher than those exhibited by Trolox. Total dry extracts of guabiju exhibited greater inhibition of chemotaxis at a concentration of 4 μg/mL, except for GBH (wild-type hydrolyzed extract) which already presented high values at a concentration of 2 μg/mL. These results suggest that the consumption of this fruit, rich in polyphenols, may be beneficial to human health. Practical Application: The paper is the first attempt on the improvement of this native fruit, since it is widely consumed regularly as part of the South American diet. The content of phenolic compounds demonstrates that consumption of guabiju would be beneficial to human health. Differences among samples, originating from open pollination of plants growing on the same site, lead to the conclusion that improvements can be made in the chemical composition and beneficial activity of guabiju fruits by simply selecting genotypes for these characteristics among open-pollinated seedlings.

The Rubiaceae family is composed mostly of trees, shrubs, or infrequently herbs and comprises about 450 genera and 6.500 species with a complex taxonomy. For Psychotria leiocarpa Cham. & Schltdl., leaves were analyzed for their alkaloid and iridoid content [1][2][3][4][5]. A number of studies have reported different pharmacological effects of Psychotria alkaloids, such as inhibition of the aggregation of human platelets [6], cytotoxicity [7], and analgesic activity [8]. The crude ethanolic extract of P. leiocarpa leaves yielded a nonspecific analgesic activity in the tail flick test [9]. To the best of our knowledge, there are no previous studies dealing with the volatiles of this species. As part of our research on Rubiaceae species [1-5], we now report the chemical composition of the essential oil.Leaves of P. leiocarpa were collected in Osorio, Rio Grande do Sul, South Brazil, in July 2008. A voucher specimen (Carlos 6) was deposited at the herbarium of the Universidade Federal do Rio Grande do Sul (ICN, SOBRAL 7898).The oil was obtained from 100 g of fresh leaves by hydrodistillation using a Clevenger-type apparatus for four hours. The oil obtained had a yield of 0.1%.Analysis of the oil was performed using a Shimadzu GC-17A equipped with Shimadzu GC 10 software, using a DB-5 fused silica capillary column (25 m, 0.25 mm; film thickness 0.25 Pm). The oven temperature was programmed from 60-300qC at 3qC/min, with helium as carrier gas at an inlet pressure of 80 kPa (1 mL/min). Injector (split mode) and detector temperatures were set at 220qC and 250qC, respectively. The percentage compositions were obtained from electronic integration measurements using flame ionization detection (FID, 250qC). The sample was analyzed by GC/MS in the same chromatographic conditions as described above, using a Shimadzu QP-5000-quadrupole MS system, operating with an ionization energy of 70 eV and an interface temperature of 250qC, with a scan time of 1.25 scans/s and an acquisition mass range of 40.0-650.0 Da.The identification of the oil components was accomplished by comparison of their GC retention indices as well as their mass spectra with corresponding data of authentic compounds or of components of reference oils; some mass spectra were compared with those of the NIST (National Institute of Standards and Technology) computer data library or with spectral data given in the literature [10][11][12][13].Thirty-three compounds were identified, comprising 95.9% of total volatiles for Psychotria leiocarpa leaves ( Table 1). The essential oil was characterized exclusively by sesquiterpenes. P. leiocarpa oil consists of a large amount of sesquiterpene hydrocarbons with germacrane and cadinane skeletons, such as bicyclogermacrene (35.6%) and germacrene D (17.6%), respectively.

No abstract

Objectives Evaluating the effects of rosmarinic (RA) and cryptochlorogenic (CGA) acids isolated from Blechnum binervatum extract on stem cell viability, toxicity and the protective effect on oxidative cell damage. Methods MTT and LDH methods were employed, using stem cells from teeth. RA and CGA were evaluated at 100, 250 and 500 µM. The negative effect of hydrogen peroxide (H2O2) (200–2200 µM) and the capacity of RA and CGA (10–100 µM) as protective agents were also evaluated. DAPI followed by fluorescent microscopy was employed to photograph the treated and untreated cells. Key findings At all tested concentrations, RA and CGA demonstrated the ability to maintain cell viability, and with no cytotoxic effects on the treated stem cells. RA also induced an increase of the cell viability and a reduction in cytotoxicity. H2O2 (1400 µM) induced >50% of cytotoxicity, and both compounds were capable of suppressing H2O2 damage, even at the lowest concentration. At 100 µM, in H2O2 presence, total cell viability was observed through microscope imaging. Conclusions These findings contribute to the continued research into natural substances with the potential for protecting cells against oxidative injury, with the consideration that RA and CGA are useful in the regeneration of damaged stem cells.

No abstract