Rosmarinic and chlorogenic acid, isolated from ferns, suppress stem cell damage induced by hydrogen peroxide

Abstract

Objectives Evaluating the effects of rosmarinic (RA) and cryptochlorogenic (CGA) acids isolated from Blechnum binervatum extract on stem cell viability, toxicity and the protective effect on oxidative cell damage. Methods MTT and LDH methods were employed, using stem cells from teeth. RA and CGA were evaluated at 100, 250 and 500 µM. The negative effect of hydrogen peroxide (H2O2) (200–2200 µM) and the capacity of RA and CGA (10–100 µM) as protective agents were also evaluated. DAPI followed by fluorescent microscopy was employed to photograph the treated and untreated cells. Key findings At all tested concentrations, RA and CGA demonstrated the ability to maintain cell viability, and with no cytotoxic effects on the treated stem cells. RA also induced an increase of the cell viability and a reduction in cytotoxicity. H2O2 (1400 µM) induced >50% of cytotoxicity, and both compounds were capable of suppressing H2O2 damage, even at the lowest concentration. At 100 µM, in H2O2 presence, total cell viability was observed through microscope imaging. Conclusions These findings contribute to the continued research into natural substances with the potential for protecting cells against oxidative injury, with the consideration that RA and CGA are useful in the regeneration of damaged stem cells.

Chemical and Biological Strategies for Profiling Protein‐Protein Interactions in Living Cells

“…For example, it was found that alkyne‐phenol not only had enhanced cell membrane permeability, but also the labeled proximal protein with an alkyne handle can be further derivatized with biotin‐conjugated azide using click chemistry reaction for visualization and enrichment [64] . Despite these advances, the greatest potential drawback of peroxidases is the inevitable cell damage and the disturbance to redox‐sensitive pathways caused by hydrogen peroxide [65] . Although free radical quenchers, including sodium ascorbate and trolox, and peroxidase inhibitor such as sodium azide are commonly used to terminate the labeling reaction to avoid excessive oxidation, [64] cautions need to be taken that these additives can still cause cell damage in certain ways [66]…”

“… [64] Despite these advances, the greatest potential drawback of peroxidases is the inevitable cell damage and the disturbance to redox‐sensitive pathways caused by hydrogen peroxide. [65] Although free radical quenchers, including sodium ascorbate and trolox, and peroxidase inhibitor such as sodium azide are commonly used to terminate the labeling reaction to avoid excessive oxidation, [64] cautions need to be taken that these additives can still cause cell damage in certain ways. [66]…”