The Effect of Rosmarinus Officinalis as a Potential Root Canal Medication on the Viability of Dental Pulp Stem Cells

Elhawary, Yousry Mahmoud; Badr, Amany Elsaid; Othman, Nesma Mohamed; Elbeltagy, Mohamed G

doi:10.5005/jp-journals-10024-3570

Cited by 6 publications

(1 citation statement)

References 44 publications

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“…With rising microbial antibiotic resistance, researchers are exploring new treatment options for oral disorders. 20 Various herbal medicaments like glycyrrhizin 21 and Rosmarinic acid (RA) 22 are being investigated as alternative intracanal treatments, creating an ideal microenvironment for regenerative endodontics due to their biocompatibility and antibacterial, anti-inflammatory, and antioxidant effects. Compounds like acetyl-11-keto-β-boswellic acid, 23 and Asiatic acid 24 also show promise in enhancing collagen synthesis and stimulating mesenchymal stem cell osteogenic differentiation, making them potential solutions to traditional materials' limitations like Ca(OH) 2 and TAP in promoting dentin-pulp complex regeneration.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of the Effect of Oleanolic Acid as a Potential Root Canal Medicament on Viability and Proliferation of Dental Pulp Stem Cells

Alhaila,

Badawi,

Elbeltagy

et al. 2024

European Journal of General Dentistry

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Objective In light of the potential drawbacks associated with certain intracanal medicaments, such as triple antibiotic paste (TAP) and calcium hydroxide (Ca(OH2)), the introduction of herbal agents has ushered in a new era in the field of dentistry. Consequently, this study aimed to explore the impact of oleanolic acid (OA) on the viability and proliferation of dental pulp stem cells (DPSCs), comparing its effects to those of conventional intracanal medicaments, TAP and Ca(OH2). Materials and Methods DPSCs were derived from the third molars of an adult donor. Flow cytometry was utilized to do a phenotypic study on DPSCs. The methyl-thiazol tetrazolium (MTT) test was used to evaluate cellular viability. The cells were subjected to various concentrations of TAP and Ca(OH)2 (5, 2.5, 1, 0.5, and 0.25 mg/mL), in addition to OA (40, 20, 10, 5, and 2.5 µM). A cell proliferation experiment assessed the cell growth precisely at 3, 5, and 7 days. Results DPSCs were characterized by flow cytometry. The mesenchymal markers (CD73, CD90, and CD105) had a positive expression. However, the hematological markers (CD14, CD34, and CD45) showed negligible expression. A notable reduction in cellular viability was seen in cells subjected to concentrations exceeding 0.5 mg/mL of TAP and Ca(OH)2 compared to the cells that were not treated (p < 0.05). The cells treated with different concentrations of OA 2.5, 5, 10, and 20 µM did not exhibit any significant variance in cell viability compared to untreated cells (p > 0.05). Moreover, the concentrations of OA (20, 10, and 5 µM) showed high proliferation level compared to TAP and Ca(OH2) especially 5µM of OA after 7 days (p < 0.05). Conclusion Our results revealed that OA exerted significant effect on the viability and proliferation of DPSCs compared to TAP and Ca(OH2).

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Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of the Effect of Oleanolic Acid as a Potential Root Canal Medicament on Viability and Proliferation of Dental Pulp Stem Cells

Alhaila,

Badawi,

Elbeltagy

et al. 2024

European Journal of General Dentistry

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Rosmarinus officinalis Linn.: unveiling its multifaceted nature in nutrition, diverse applications, and advanced extraction methods

Meziane,

Zraibi,

Albusayr

et al. 2024

J.Umm Al-Qura Univ. Appll. Sci.

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Rosemary (Rosmarinus officinalis Linn. or Salvia rosmarinus Spenn.) is an aromatic herb renowned for its culinary, medicinal, and industrial significance. This review offers a multifaceted exploration of rosemary, delving into its nutritional composition, traditional culinary applications, and historical uses in traditional medicine. The review extends to cosmetic and pharmaceutical applications, elucidating rosemary’s role as a natural preservative and its integration into cosmeceutical and pharmaceutical formulations. Extraction methods, both classical and contemporary, are critically examined, with an emphasis on recent sustainable approaches and their impact on bioactive compounds. This work concludes with a forward-looking perspective, discussing innovative extraction techniques, advanced technologies, and the potential commercial viability of rosemary-related industries. This comprehensive review serves as a valuable resource, offering insights into the diverse dimensions of rosemary, from traditional applications to cutting-edge advancements in extraction science.

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New Perspectives about Relevant Natural Compounds for Current Dentistry Research

Dinu,

Dumitrel,

Buzatu

et al. 2024

Life

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Natural compounds have been used since the earliest civilizations and remain, to this day, a safer alternative for treating various dental problems. These present antimicrobial, anti-inflammatory, antioxidant, analgesic, and antimutagenic effects, making them useful in the prophylactic and curative treatment of various oral diseases such as infections, gingivitis, periodontitis, and even cancer. Due to the high incidence of unpleasant adverse reactions to synthetic compounds, natural products tend to gradually replace conventional treatment, as they can be just as potent and cause fewer, milder adverse effects. Researchers use several methods to measure the effectiveness and safety profile of these compounds, and employing standard techniques also contributes to progress across all medical disciplines.

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The Effect of Rosmarinus Officinalis as a Potential Root Canal Medication on the Viability of Dental Pulp Stem Cells

Cited by 6 publications

References 44 publications

In Vitro Evaluation of the Effect of Oleanolic Acid as a Potential Root Canal Medicament on Viability and Proliferation of Dental Pulp Stem Cells

In Vitro Evaluation of the Effect of Oleanolic Acid as a Potential Root Canal Medicament on Viability and Proliferation of Dental Pulp Stem Cells

Rosmarinus officinalis Linn.: unveiling its multifaceted nature in nutrition, diverse applications, and advanced extraction methods

New Perspectives about Relevant Natural Compounds for Current Dentistry Research

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