O objetivo deste artigo é propor uma abordagem teórico-metodológica em Psicologia que parte da articulação entre duas áreas do conhecimento: a Análise do Discurso pêcheutiana (AD) e a Psicanálise lacaniana. Em contraste com o paradigma galileano, essas áreas são tomadas com base no paradigma indiciário (Ginsburg,1986), pelo fato de partirem de pistas para chegar aos efeitos do discurso e do inconsciente. A análise se dá a partir da mobilização de um dispositivo teórico que se organiza em função do corpus e constitui o dispositivo analítico. São mobilizados conceitos da AD, e a Psicanálise comparece como parte do dispositivo teórico, considerando a dimensão inconsciente na análise. Para ilustrar, apresentamos um fragmento de análise que articula as duas áreas.
The hepatitis C virus (HCV) is an enveloped virus that is about 50-70 nm in diameter, has positive-strand RNA, and belongs to the genus Hepacivirus and the family Flaviridae. The detection and quantification of the core antigen, HCV nucleocapsid protein, has been successful in many trials and is considered a marker of viral replication since it presents a sequence of highly conserved amino acids, giving it high sensitivity and specificity. The E2 protein is an envelope glycoprotein of HCV with 11 glycosylation sites; most of these are well-conserved, making it a target antigen. The aim of this study is to develop high-sensitivity, low-cost diagnostic methods for HCV, which could be used for serological screening. The genomic regions encoding the core (part 136 aa) and E2 proteins of HCV were expressed in Escherichia coli Rosetta strain, cloned in expression vector pET-42a, and induced with 0.4 m mol L(-1) IPTG, producing recombinant proteins that were fused to glutathione S-transferase (GST) protein, which was then purified by affinity chromatography. The immunoreactivity was assessed by Western blot, Slot Blot, and developed and improved diagnostic methods (capture, indirect, and immunoblotting enzyme-linked immunosorbent assay (ELISA)). After applying the results to the formulas for determining the quality parameters, obtained for immunoblotting method 100% sensitivity and specificity and for ELISA 100% sensitivity and 87.5% specificity. The methods developed were more sensitive and specific using the mixture of the recombinant proteins fused to GST (core+E2).
Introduction: During aging, significant declines in muscle strength decrease people's functional capacity. In this context, Concurrent Training (TC in Portuguese abbreviation) can lead to improvements in muscle strength and cardiorespiratory capacity. However, there are few studies on the influence of the performance order of this training on the variables of static and dynamic balance. Objective: To evaluate the effect of the performance order of TC, Aerobic-Strength and Strength-Aerobic (respectively AF and FA in Portuguese abbreviation), on the static and dynamic balance, as well as muscle strength of elderly people. Material and Methods: 38 elderly men and women participated in the study, who were divided into two groups: AF (n=19) and AF (n=19). The training was carried out during 12 weeks, in which the aerobic training consisted of walking with intensity prescribed by the Borg Scale of Perceived Exertion (PE) (6-20) and the strength training consisted of six exercises, with intensity controlled by zones of Maximum Repetitions (RM, in Portuguese abbreviation). Static (area and displacement of plantar pressure center in bipedal support with eyes closed and open) and dynamic (Timed Up and Go and Tandem March) balance were evaluated, as well as maximum dynamic force of knee extension and bench press. For the data analysis, the Generalized Estimation Equations were used with Bonferroni's complementary test (α=0.05).Results: For the static and dynamic balance, there was no effect of the 12 weeks of combined training, regardless of the order of execution. This result demonstrates that both groups presented a maintenance of equilibrium variables during the intervention period. In strength, there was an improvement in the lower limbs (FA: 16%; FA: 11%; p<0.001) and upper (FA: 22.0%; FA: 8, 7%; p<0.001), with no difference between groups. Conclusion: There is no difference between the performance order of TC in the variables of static and dynamic balance and strength of upper and lower limbs. However, after training, significant advances were considered in the variables of strength and maintenance of static and dynamic balance.
Assess the effect of the performance order in the Concurrent Training (CT), Aerobic-Strength (AS), and Strength-Aerobic (SA), in the static balance, dynamic balance, and muscle strength in elderly people. Methods:The study involved 38 elderly people (men and women) aged 60 to 75 years old, divided into SA (n = 19) and AS (n = 19). Within 12 weeks, the aerobic training consisted of walking with intensity prescribed by the Borg's Rating of Perceived Exertion (6-20) and the strength training consisted of six exercises, with intensity controlled by Repetition Maximum training zones. Static balance (plantar pressure center area and displacement in bipedal support with eyes closed and open), dynamic balance (Timed Up and Go and Tandem Gait), and maximum dynamic strength of knee extension and bench press have been evaluated. For data analysis, Generalized Estimating Equations with Bonferroni's complimentary test have been used (α = 0.05). Results: For static and dynamic balance there hasn't been an effect on the 12 weeks of combined training, regardless of the performance order. Both groups maintained the balance variables within the intervention period. When it comes to strength, there has been a noticeable improvement in lower limbs (SA: 16%; AS: 11%; p < 0,001) and upper (SA: 22.0%; AS: 8.7%; p < 0.001), without any differences between the groups.
Conclusion:So there is no difference between the order of performance of the CT in the variables of static and dynamic balance and strength of upper and lower limbs. Furthermore, after training, there have been significant improvements in the variables of strength and maintenance of static and dynamic balance.
Resumo O projeto de pesquisa identifica e analisa as tendências teórico-metodológicas na produção acadêmica, no que tange à temática identidade docente e disciplina escolar, por meio da construção de um banco de dados. Metodologicamente baseamos no Portal de Periódicos da Capes (conteúdo assinado pela Unicamp). Primeiramente realizamos um levantamento bibliográfico, em que identificamos setenta produções. Em segunda etapa elaboramos resumos (modelo tipo REDUC) dos trabalhos selecionados no levantamento bibliográfico e, por fim identificamos e delimitamos as tendências teórico-metodológicas presentes neles.
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