Background:Regarding to the data generated by Basic Health Research in 2018, the prevalence of Indonesian people with dental and oral problems was amounted to 57.6%. Haruan fish scale chitosan has been shown to be capable of inhibiting growth and killing microorganisms that cause oral disease and has the potential to become a safe biomaterial. Toxicity testing was carried out to ensure safety of the chemical compounds before they can be used as drugs or during clinical trials. Purpose:This research was intended to analyze the toxicity of haruan fish scales (Channa striata) chitosan on BHK-21 fibroblast cells by utilizing the MTT assay method. Method:This research was classified as a true experimental research by adopting a post-test-only design with a control group design consisting of 6 groups. The treatment of haruan fish scale chitosan was given to 4 groups with concentrations of 25%, 50%, 75%, and 100%, with 2 control groups, specifically cell control and media control. The absorbance value was read by means of ELISA reader and the percentage of cell viability was subsequently calculated. The results of the average percentage of cell viability were then analyzed using SPSS to obtain the IC50 value. Result:The calculation of the average cell viability of each concentration was successfully obtained in this research. The average cell viability at 25% concentration was 2.106%, at 50% concentration was 12.01%, at 75% concentration was 77%, and at 100% concentration was 80.194%. The average percentage of cell viability data was further entered into the SPSS software and the value of IC50> 1000 µg/mL was successfully obtained. Conclusion: Haruan fish chitosan (Channa striata) is not toxic to BHK-21 fibroblas cell. Keywords : Channa striata , Fibroblast cell, Scale chitosan, Toxicity test.
Background: One of the causes of tooth nerve death or pulp necrosis is microorganisms, namely is Actinomyces spp. Ramania leaf extract (Bouea Macrophylla Griff) contains secondary metabolites, namely triterpenoids, alkaloids, saponins, tannins, flavonoids, steroids, and phenolics which function to inhibit the growth of Actinomyces spp. Purpose: This study aims to analyze the antibacterial activity of ramania leaf extract (Bouea Macrophylla Griff) at concentrations of 6,25%, 12,5%, 25%, 50%, and 100% against the growth Actinomyces spp. Methods: This research is a pure experimental (true experimental), with Posttest Only with Control Group Design. Antibacterial activity test used a paper disc diffusion with seven treatments, namely ramania leaf extract (Bouea Macrophylla Griff) with a concentration of 6,25%, 12,5%, 25%, 50%, and 100%, Chlorhexidine gluconate 2% as a positive control and Aquades as a negative control of Actinomyces spp were repeated 5 times. Measuring the diameter of the clear zone formed on Mueller Hinton Agar (MHA) media using a caliper. Results: Based on the results of the Mann Whitney test, there was 1 pair of groups that did not differ significantly in inhibiting Actinomyces spp bacteria, namely the 25% concentration group with a positive control of 2% Chlorhexidine gluconate, while the other groups had significant differences from each other. Conclusion: There is antibacterial activity in ramania leaf extract (Bouea Macrophylla Griff) against Actinomyces spp. Keywords: Actinomyces spp, Antibacterial Activities, Chlorhexidine gluconate 2%, Ramania Leaf Extract.
Background: Badan Penyelenggaran Jaminan Sosial (BPJS) is a form of the implementation of the National Health Insurance which is tasked to ensure the maintenance of health for all Indonesians. But many people are not satisfied with BPJS. Kurniawan research in 2015, treatment measures (aspects of assurance) were significantly different between uninsured patients and BPJS patients. Idaman Hospital, Banjarbaru is a type C hospital owned by the city government, as an advanced level health facility in implementing BPJS. Pulp treatment is a kind of treatments in dental and mouth which covered by BPJS. Purpose: To know the description of pulp treatment and post pulp treatment materials in BPJS and uninsured patients in Idaman District Hospital, Banjarbaru. Methods: A descriptive observational study with retrospective secondary data collect from patient registers in January-December 2018. Results: Outpatients that insured by BPJS were 78.84% and uninsured were 21.10%. The use of dental pulp treatment materials was arsenic with 13.6% in BPJS patients and 6.6% in uninsured patients, formocresol with 21.5% in BPJS patients and 5.6% in uninsured patients, cresophene 20.42% in BPJS patients and 3.76% in uninsured patients, eugenol 2,81% in BPJS patients and 2.35% in uninsured patients. The most common used post-pulp treatment materials were 16.6% GIC for BPJS patients and 6.09% in uninsured patients, while composites were only 0.23% for both. Conclusion: Dental pulp treatment materials in BPJS and uninsured patients are arsenic, formocresol, cresophene and eugenol, while post pulp treatment materials are GIC and composite.Keywords: BPJS, Pulp Treatment, Uninsured Patients
BACKGROUND: Case fatality rate (CFR) for global COVID-19 infections since June 14, 2021 was 2.17%, while CFR for Southest Asia were 1.39%. CFR in Indonesia so far were 3.05%. This missed from the target of the 2005-2025 RPJMK (Middle long run national health planning) in achieving healthy Indonesia; handling epidemic diseases must be able to reduce the mortality rate below 1%. The government issued the Decree of the Minister of Health Republic of Indonesia No. HK.01.07/Menkes/2020 concerning the Determination of Vaccine Types for the Management of COVID-19. However, the existence of this policy did not reduce the mortality rate trend of COVID-19 in Indonesia. Hypertension and diabetes mellitus were the larger risk factors for COVID-19 mortality. Guo et al. 2020 found comorbid COVID-19 sufferers were hypertension 24.7% and diabetes mellitus 21.2%. However, Mikami et al., 2020 stated differently that hypertension and diabetes mellitus were not at risk of COVID-19 mortality. AIM: Objective of this study was to estimate the average tendency of hypertension and diabetes mellitus as risk factor for COVID-19 mortality. METHODS: Meta-analysis with 16 articles analyzed by RevMan 5.4. RESULTS: pHR for hypertension was 1.15 (95% CI 1.00 - 1.32) and diabetes mellitus was 1.21 (95% CI 1.13 - 1.29). CONCLUSION: Hypertension had risk 1.15 times and diabetes mellitus had risk 1.21 times for COVID-19 mortality.
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