High copper (Cu) soil contents, due to the continuous vineyard application of Cu fungicides throughout the years, may impair the growth of the shoot and modify the structure of the root system. The current study aimed to investigate the threshold levels of available Cu in the soil causing toxicity effects in young grapevine plants of 'Red Niagara' cultivated in clay soils. Grapevine plantlets were cultivated in pots containing vineyard devoted soils with increasing contents of available Cu (25, 80, 100 and 165 mg kg), for 53 days. Photosynthesis and transpiration rates, and the quantum yield of photosystem II (Fv/Fm) were evaluated during the cultivation period. At the end of the experiment, the plant nutrient and leaf chlorophyll were determined, along with the anatomical analysis of the root system structure and plant dry matter determination. Higher levels of available Cu in the soil increased the apoplastic, symplastic and total fraction of the metal in the roots, reducing the other nutrients, especially in the shoots. Photosynthesis, transpiration rates and Fv/Fm were also reduced. Higher levels of Cu led to anatomical changes in the roots, that increased diameter, number of layers in the cortex, vascular cylinder and total root areas. It also resulted in reduced dry matter production by grapevines.

NUTRITIONAL STATUS, VIGOR AND YIELD OF GRAPEVINES INTERCROPPED WITH COVER CROPSABSTRACT -Perennial or annual species of cover crops can be grown in vineyards to protect the soil surface and to reduce the availability of water and nutrients to the vine. The study aimed to evaluate the nutritional status, vigour and yield of grapevines intercropped with some species of cover crops under two different managements. A field experiment was conducted for two years, 2009 through 2011, in a vineyard of cv Cabernet Sauvignon in a Haplumbrept soil. The following treatments were evaluated: a control, where weeds were controlled by chemical drying in the grapevine row and by mowing in the inter row; a perennial species, tall fescue (Festuca arundinacea); two sequences of annual species, ryegrass-moha (Lolium multiflorum-Setaria italica) and oat-buckwheat (Avena sativa-Fagopyrum esculentum); and two types of crop residues management, with and without its transfer of the grapevine row to the inter row. The grapevine leaves were sampled at flowering and at berries softening stages for analysis of N, P, K, Ca and Mg contents. It was determined the branches and internodes lengths, branches pruned mass, grape bunches length, width and mass, yield per plant and the weight of 100 berries and it was also calculated the Ravaz index of the grapevines. Grapevines intercropped with succession of annual cover crops showed higher total N content in leaves at flowering, vigour and grape yield. The transfer of the crop residue from the row to the inter row, did not affect the grapevine vigour and yield, but decreased the total content of N in the leaves at flowering. The tall fescue as soil cover crop in vineyard can be an effective alternative to reduce the vigor of the grapevine.

Resumo -O objetivo deste trabalho foi avaliar a influência de plantas de cobertura verde sobre a produtividade das videiras e sobre a composição da uva e do vinho. Durante duas safras, foram feitas avaliações de três tipos de consórcio, dois manejos das coberturas e de um tratamento controle, com plantas espontâneas controladas por herbicidas e roçagem. Utilizou-se vinhedo de uvas 'Cabernet Sauvignon', localizado a 1.130 m de altitude, em um Cambissolo Húmico distrófico, em São Joaquim, SC. Os consórcios foram realizados com a sucessão de cultivos anuais de moha (Setaria italica) com azevém (Lolium multiflorum) e de trigo mourisco (Fagopyrum esculentum) com aveia-branca (Avena sativa), bem como com a planta perene festuca (Fetusca sp.). Os manejos consistiram da transferência ou não do resíduo cultural da linha para a entrelinha. As videiras apresentaram maior produtividade de uva no consórcio com as plantas anuais, em comparação ao tratamento controle, ou com a planta perene festuca. O manejo da cobertura verde não teve influência sobre as variáveis avaliadas. Os consórcios não influenciaram de forma consistente os teores de N da uva nem a composição do mosto, embora, na última safra, o teor de sólidos solúveis totais do mosto tenha sido maior nos tratamentos com consórcio, em comparação ao controle. Além disso, as videiras consorciadas com festuca podem proporcionar vinho com maior teor de antocianinas e polifenóis totais.Termos para indexação: Vitis vinifera, adubo verde, qualidade da uva, vigor vegetativo, vinho de altitude. Productivity and composition of grapes and wine of vines intercropped with cover cropsAbstract -The objective of this work was to evaluate the influence of green cover crops on vine productivity and on grape and wine composition. For two growing seasons, evaluations were done for three intercrops, two managements of the cover crops, and for a control treatment with weeds controlled by herbicides and mowing. A vineyard of 'Cabernet Sauvignon', located at 1,130 m altitude in a Haplumbrept soil, in São Joaquim, SC, Brazil was used. Intercropping was done with a succession of the cover crops moha (Setaria italica) with annual ryegrass (Lolium multiflorum), and buckwheat (Fagopyrum esculentum) with oat (Avena sativa), and with perennial fescue (Fetusca sp.). Cover crop managements consisted of transferring or not crop residues from the row to the between rows. Grape yield were higher in vines intercropped with the annual plants, in comparison to the control treatment or to fescue. The management of the cover crops did not affect the evaluated variables. Intercropping did not consistently influence N contents of grapes and wine composition; however, soluble solid contents of must were greater with intercropping treatments compared to control, in the last season. Moreover, vines intercropped with fescue can enable wine with greater content of anthocyanins and total polyphenols.

RESUMO As aplicações sucessivas de dejeto líquido de suínos podem aumentar os teores de cobre e fósforo na

ResumoA aplicação de calcário pode diminuir a toxidez de Cu em videiras jovens. O trabalho objetivou avaliar o efeito da calagem em solos com adição de Cu como estratégia de amenizar a toxidez do metal pesado em videiras jovens. O experimento foi conduzido em casa de vegetação utilizando solo coletado em uma área sem histórico de cultivo. O solo foi dividido em três porções iguais, que receberam doses de calcário correspondentes a 0, 12,6 e 25,2 Mg ha -1 . Após 45 dias de incubação, o solo recebeu doses crescentes de Cu: 0, 50, 100, 200 e 300 mg kg -1 de solo, aplicadas na forma de sulfato de Cu. Posteriormente, o solo foi incubado novamente por 45 dias. Em vasos contendo três quilogramas de solo, foram transplantadas estacas de videira 'Niágara Rosada'. Após 103 dias de cultivo, os ramos das videiras foram podados e descartados. Aos 46 dias após a primeira poda, foram determinadas as clorofilas a e b (Índice de Clorofila Falker -ICF). O cultivo foi finalizado aos 77 dias após a primeira poda, quando foi determinada a área foliar e foi coletado material vegetal para a determinação da matéria seca (MS) de raízes e da parte aérea, e o acúmulo de N, P, K, Ca, Mg e Cu em cada parte. O aumento das doses de Cu reduziu a MS de raízes e da parte aérea, a área foliar, as clorofilas a e b (ICF) e a absorção de todos os nutrientes nas videiras jovens. A calagem minimizou os efeitos causados pelo excesso de Cu.Palavras-chave adicionais: absorção de nutrientes; calagem; metal pesado; Vitis sp. AbstractLiming can reduce the toxicity of Cu in young vines. The study aimed to evaluate the effect of liming on soils with addition of Cu, as strategy to mitigate the toxicity of heavy metal in young vines. The experiment was conducted in a greenhouse using soil collected in area without history of cultivation. The soil was divided into three equal portions, which received doses of lime corresponding to 0, 12.6 and 25.2 Mg ha -1

Cover crop nitrogen (N) cycling has an important role in agricultural production and contributes to peach [Prunus persica (L.) Batsch] N nutrition. This study evaluated black oat (Avena strigosa Schreb) and ryegrass (Lolium multiflorum L.) residue decomposition dynamics, N recovery from cover crop residues, and N compartmentalization in peach tree organs. A 2-year field trial was developed with labeled (3.6-4.0 atom% 15 N excess) cover crop shoot biomass application in a 5-year-old peach orchard. The region's climate is warm temperate (Cfb), and the soil is classified as a Typic Hapludalf. Litter bags with unlabeled shoot residues were also deposited in the orchard to assess biomass, carbon (C), N, lignin, cellulose, and non-structural biomass decomposition dynamics. After 13 months, the leaves, trunk, and roots showed the greatest proportion of N derived from residues (Ndfr) (35.4, 25.1, and 22.4%, respectively) while the greatest concentrations of 15 N and Ndfr occurred in roots <2 mm (0.0376 and 0.94%, respectively). The N derived from cover crop shoots in the second production cycle was similar among tree organs. Ryegrass residues presented the highest decomposition constant (k) values for dry matter, total organic carbon (TOC), cellulose, and lignin. Hence, black oat residues presented a higher half-life (t ½ ) for dry matter, TOC, total N, cellulose, and lignin. The N derived from black oat and ryegrass residues in mature trees was expressively low (<1%) and similar between species. Within organs, the highest Ndfr occurred in peach leaves during the flowering stage, when the greatest residue decomposition rate also occurred. Soil N and plant internal N reserves are the major N sources for newly formed organs, but greater contributions to tree N nutrition may occur with long-term cover crop residue deposition and different plant species.

RESUMOO cultivo de cobertura verde em vinhedos protege o solo da erosão e ainda pode ser uma alternativa para o controle do vigor da videira. Assim, o objetivo desse estudo foi avaliar a influência do cultivo de plantas de cobertura do solo de vinhedo nos atributos químicos do solo, na composição foliar e no crescimento de ramos da videira do cultivar Cabernet Sauvignon. As avalições foram realizadas na safra 2013/14, em experimento conduzido a partir de 2009 na região do Planalto Sul Catarinense, onde as condições edafoclimáticas favorecem o excesso de vigor da videira. Os tratamentos compreenderam três tipos de plantas, dois manejos e um controle. As plantas foram a sucessão de anuais, trigo mourisco (Fagopyrum esculentum) e azevém (Lolium multiflorum), a perene festuca (Festuca arundinacea) e as nativas (espontâneas) e os manejos foram roçada com o resíduo cultural distribuído em área total e, com a sua transferência da faixa da linha da videira para a faixa da entrelinha. O controle foi a aplicação de herbicida na faixa da linha e roçada na entrelinha. As amostragens das folhas e do solo foram realizadas nas fases de mudança de cor e de maturação plena da uva. As plantas de cobertura e seu manejo, em geral, não afetaram o pH, nem os teores de Al, Ca e Mg trocáveis no solo, porém as anuais aumentaram o P extraível na camada 0 -5 cm relativamente à testemunha. Em geral, a espécie perene aumentou o K extraível relativamente à testemunha nas camadas 5-10 e 10-15 cm e relativamente às plantas anuais e nativas em todas as camadas. A festuca promoveu menor teor de Mg nas folhas da videira em relação às plantas anuais e menores teores de P e K relativamente aos demais tratamentos e também diminuiu o teor de N foliar e a massa de ramos da videira em relação à testemunha. PALAVRAS-CHAVE:Cabernet Sauvignon, adubo verde, atributos do solo, composição foliar. ABSTRACTThe cover crop intercropping in vineyards protects the soil from erosion and can be an alternative for controlling the growth of the vine. The aim of this study was to evaluate the changes in the soil's chemical properties, leaf composition and branch growth of Cabernet Sauvignon vine caused by the cover crops in the vineyard. The study was carried in an experiment conducted from 2009 in the highlands of Santa Catarina state, where the soil and climatic conditions favor the excessive growth of the vine. The treatments consisted of three types of cover crops, two management and a control. The cover crops were annual, buckwheat (Fagopyrum esculentum) and ryegrass (Lolium multiflorum), the perennial fescue (Festuca arundinacea) and the native (spontaneous), while the managements were mowed with the crop residue in total area and, with its transfer from the row to the between row of the vine. Samples of leaves and soil were carried out in "veraison" and at end of grape maturation stages, respectively. The cover crops and its management in general did not change the soil pH, or the exchangeable content of Al, Ca and Mg, but the annual did increase the available P...

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