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Several cover crops (CCs) exert allelopathic effects that suppress weed growth. The aim of the present study was to evaluate the effects of aqueous extracts containing different concentrations [0, 0.5, 1, 2.5, 5, 7.5 and 10% (w/v)] of Brassicaceae CCs (Sinapis alba, Raphanus sativus, Camellina sativa) and of the CCs Fagopyrum esculentum and Guizotia abyssinica on germination and early growth of Ambrosia artemisiifolia L. The allelopathic effects were species and concentration-dependent. C. sativa, for example, caused the greatest potential to inhibit germination, shoot, radicle length and fresh seedling weight, whereas S. alba and R. sativus inhibited germination and early growth of A. artemisiifolia only at concentrations ≥ 7.5%. In contrast, no inhibition was observed when aqueous extracts of F. escultneum and G. abyssinica were added at any of tested concentration. Liquid chromatography-tandem mass spectrometry detected 15 phenolic compounds in Brassicaceae CCs with the highest content (µg/g) of vanillin (48.8), chlorogenic acid (1057), vanilic acid (79), caffeic acid (102.5) and syringic acid (27.3) in C. sativa. Our results suggest that C. sativa is the most allelopathic CCs and that the fruits of C. sativa are the plant organs richest in allelochemicals.

Herbicide residues can potentially injure sensitive crops grown in rotation. Thus, the objective of this study was to evaluate the sensitivity of six replacement crops to mesotrione residues 1 yr after herbicide application. In field bioassay, mesotrione was applied at recommended (144 g ai ha-1), twofold (288 g ai ha-1), and fourfold (576 g ai ha-1) rates at two soil types (Gleysol and Fluvisol). In field and laboratory bioassays, mesotrione residual activity was followed for a 21-d period using various measurements of phytotoxicity. No visible injuries to mesotrione residues were observed on oat (Avena sativa L.), rapeseed (Brassica napus L.), soybean (Glycine max [L.] Merr.) and sunflower (Helianthus annuus L.) in the field bioassay. Although mesotrione residues were not detected by HPLC-UV/DAD analysis, field bioassays indicated their presence due to visible injuries on field pea (Pisum sativum L.) grown in Gleysol soil with twofold and fourfold herbicide treatments. In contrast to other test crop responses, sugar beet exhibited visible injuries in both soils, and consequently, was subjected to laboratory bioassay. With increasing mesotrione rates, the reductions in sugar beet (Beta vulgaris L. var. saccharifera Alef.

Variations in soil pH have been shown to affect mesotrione adsorption, which in turn, may have an impact on crop susceptibility. Therefore, a greenhouse experiment was conducted to evaluate the effect of simulated mesotrione residues on pea crop grown in the typical agricultural soil (gleysol) of north-western Croatia. The soil pH was manipulated to obtain neutral (pH 7.0) and acidic (pH 5.0) values. Simulated mesotrione residues were 1.1, 2.3, 4.5, 9.0, 18, 36 and 72 g a.i. ha−1. Crop visual injuries as well as reductions in chlorophyll fluorescence and aboveground dry biomass were higher at pH 7.0 than at pH 5.0. With increasing mesotrione residues, the reductions in chlorophyll fluorescence ranged from 38.8% to 89.7% at pH 5.0 and from 63.7% to 99.3% at pH 7.0. Compared to chlorophyll fluorescence, the reductions in dry biomass were smaller and ranged from 49.2% to 96.8% at pH 7.0 and from 32.0% to 82.6% at pH 5.0 for the mesotrione residues from 1.1 to 72 g a.i. ha−1. These results indicate that soil pH is an important factor determining the susceptibility of pea crop to simulated mesotrione residues.

Rhizobia are an important component of sustainable agricultural production. In symbiosis with legumes, they provide adequate amounts of nitrogen for their growth and leave nitrogen in the soil for crops that follow in the rotation. One of the major threats to symbiotic nitrogen fixation are herbicides. The effect of herbicides on symbiotic nitrogen fixation can be positive or negative. The positive effect is manifested in the stimulation of growth and development of rhizobia.When symbiosis is negatively affected, the efficiency of nitrogen fixation is reduced, resulting in lower legume growth and yield. Herbicides can contact rhizobia immediately after application or, in the case of persistent herbicides, later in the growing season. Herbicides may affect the rhizobia, the host plant, or the establishment and development of the symbiosis. This paper reviews previous research on the effects of herbicides on rhizobia.