BACKGROUND: Curcuma xanthorrhiza rhizomes have been demonstrated to have anticancer properties toward various types of cancer cells. The effect of C. xanthorrhiza rhizome extract (CXRE) on nasopharyngeal cancer (NPC) cells, including HONE-1 cell line has not been elucidated yet. Therefore, the effect of CXRE on the apoptosis of HONE-1 cells and its possible underlying mechanism are necessary to be explored.METHODS: C. xanthorrhiza rhizomes were minced, dried, extracted with distilled ethanol, filtered, and evaporated to produce CXRE. HONE-1 cells were seeded, starved, and treated with dimethyl sulfoxide (DMSO), Doxorubicin, or various concentrations of CXRE. Treated HONE-1 cells were stained with 4',6'-diamidino-2-phenylindole (DAPI) and the number of viable cells was counted. HONE-1 cells were also collected, lysed, and further processed for immunoblotting analysis to measure Bid activity.RESULTS: The number of viable HONE-1 cells decreased in concentration- and time-dependent manner. The number of viable cells in 50 and 250 μg/mL CXRE-treated groups were significantly lower compared with that in the DMSO-treated group after 24 h. At 48 h incubation period, the number of viable cells in 10, 50 and 250 μg/mL CXRE-treated groups were significantly lower compared with that in the DMSO-treated group. The number of viable cells in 250 μg/mL CXRE-treatment group was not significantly different compared with that in the Doxorubicin-treated group after 48 h. Bid expression levels in CXRE-treated groups were lower compared with that in the DMSO-treated group.CONCLUSION: CXRE could induce apoptosis via Bid activation, hence reducing the viability of HONE-1 cells.KEYWORDS: Curcuma xanthorrhiza, nasopharyngeal cancer, HONE-1 cells, apoptosis, Bid
Background: Curcuma xanthorrhiza Roxb. has bioactive compounds that are beneficial as anti-inflammatory. To get these compounds need to be extracted. Ethanol 70% can increase the solubility of both polar and non-polar compounds. RAW 264.7 cells with LPS induction cause an inflammatory state due to the active pro-inflammatory cytokines. To suppress the development of inflammation, a cytotoxicity test can be carried out to see the toxic nature of the extract against inflamed cells. Goals: To determine the cytotoxicity effect of 70% ethanol extract of C. xanthorrhiza on LPS-induced RAW 264.7 cells. Methods: In vitro laboratory experimental research. Preparation of extracts of C. xanthorrhiza Roxb. carried out with 70% ethanol then made concentrations of 200, 100, 50, 25, 12.5, 6.25, and 3.125 g/mL. Cytotoxicity was tested using the MTS. Untreated culture medium was used as a negative control and a positive control of diclofenac sodium. Then it was incubated for 24 hours at 37oC. After 24 hours of incubation, MTS was added to the plate and incubated again for 3 hours. The results were read using spectrophotometry. The resulting absorption is equivalent to living cells. The research data were analyzed using one-way ANOVA and Dunnet T3 tests. Results: Extracts of C. xanthorrhiza with concentrations of 200 and 100 g/mL were weakly cytotoxic and at concentrations of 50, 25, 12.5, 6.25, and 3.125 g/mL showed non-toxic effects on LPS-induced RAW 264.7 cells. Based on the IC50, C. xanthorrhiza Roxb. extract had a strong cytotoxic effect on LPS-induced RAW 264.7 cells. Conclusion: C. xanthorrhiza extract was toxic to LPS-induced RAW 264 cells.
Background: Squamous cell carcinoma (SCC) is the most common form of oral cancer. SCC treatment generally uses surgical procedures, chemotherapy, etc. Currently there are more than 60% of anticancer compounds obtained from natural ingredients, one of which is Gnetum gnemon L. (G. gnemon L.) or commonly called melinjo. Objective: To determine whether the G. gnemon L. seed ethanolic extract with concentrations of 1, 10, and 100 µg/mL could reduce viability and induce apoptosis in the HSC-3 cell line. Methods: Laboratory experimental research (in vitro) was conducted using the cell line HSC-3. Cells were treated with G. gnemon L. seed ethanolic extract with concentrations of 1, 10, and 100 µg/mL for 24 hours. Ethanol 70% was used as solvent and negative control. Doxorubicin was used as a positive control. The treated cells were analyzed using MTT assay and flow cytometry to examine changes in cell viability and apoptosis that occurred. Results: G. gnemon L. seed ethanolic extract with concentrations of 1, 10, and 100 µg/mL could significantly reduce viability and induce apoptosis in the HSC-3 cell line (p<0.05). The ability to reduce viability increased with the increase in extract concentration as many as 8,169 cells, 5,789 cells, and 3,068 cells and also induces apoptosis by 8.33 ± 0.93%, 16.55 ± 1.51%, and 28.73 ± 0.89% of cells tested sequentially. Conclusion: G. gnemon L. seed ethanolic extract with concentrations of 1, 10, and 100 µg/mL for 24 hours was able to reduce viability and induce apoptosis in the HSC-3 cell line.
Ameloblastoma, a tumor located in the jaw, grows slowly but locally invasive. Ameloblastoma expands in the jaw based on a mechanism resorbing the surrounding bone. To date, the bone resorption mechanisms of ameloblastoma are associated with the expression of receptor activator of nuclear factor (NF)-κB (RANK) ligand (RANKL), matrix metalloproteinases (MMPs), and tumor necrosis factor (TNF)-α. RANKL plays an important role in generating osteoclastogenesis. MMPs degrade the extracellular matrix. TNF-α can induce the formation of osteoclast and modulate the MMPs. In this review the bone resorption mechanism of ameloblastoma as well its signaling pathway will be disclosed.Keywords: Ameloblastoma, RANKL, MMPs, TNF-α.
Lansia adalah kelompok masyarakat berusia di atas 60 tahun dan mengalami proses penuaan yang disertai dengan beberapa perubahan seperti perubahan fisiologis, rongga mulut, psikologis, maupun sosial. Kehilangan gigi seluruh merupakan salah satu contoh perubahan rongga mulut yang terjadi dan dapat ditatalaksana dengan menggunakan gigi tiruan lengkap (GTL) yang berfungsi untuk memperbaiki fungsi estetik, mastikasi, dan fonetik. Kemampuan mastikasi merupakan hal yang penting karena berhubungan dengan kekuatan gigit, ketebalan M. masseter, kemampuan kognitif, dan kualitas hidup pada lansia. Penelitian ini bertujuan untuk menganalisis hubungan kemampuan mastikasi pemakai gigi tiruan lengkap dengan kekuatan gigit, ketebalan M. masseter, kemampuan kognitif, dan kualitas hidup pada lansia. Metode: Jenis penelitian ini adalah observasional analitik secara cross sectional dengan menggunakan kuesioner Mini Mental State Examination (MMSE) untuk menilai kemampuan kognitif, kuesioner Oral Health Impact Profile for Edentulous (OHIP-EDENT) untuk menilai kualitas hidup, masticatory performance evaluating gum untuk menilai kemampuan mastikasi, alat ultrasound portable untuk menilai ketebalan M.masseter, alat flexiforce untuk menilai kekuatan gigit. Total responden yang terlibat sebanyak 62 lansia. Hasil: Hasil penelitian dengan menggunakan uji Pearson menunjukkan hubungan antara kemampuan mastikasi pemakai gigi tiruan lengkap dengan kekuatan gigit (r=0,914), ketebalan M. masseter (r=0,783), kemampuan kognitif (r=0,791), dan kualitas hidup (r=0,909) dan dengan Spearman's rho didapatkan nilai p=0,01 dimana nilai p<0,05 yang menunjukkan hubungan signifikan. Simpulan: Terdapat hubungan antara kemampuan mastikasi pemakai gigi tiruan lengkap dengan kekuatan gigit, ketebalan musculus masseter, kemampuan kognitif dan kualitas hidup lansia.Kata kunci: lanjut usia; kemampuan mastikasi; kekuatan gigit; ketebalan musculus masseter; kemampuan kognitif; kualitas hidup; gigi tiruan lengkap Relationship between the masticatory ability of complete denture wearers with bite force, thickness of muscle masseter, cognitive function, and quality of life in elderly
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.