Microbial community has been a major part in the drug industry. They are known for their effectiveness and do not cause any undesirable effects. Exploitation of bacteria could be fruitful for mankind. Bacteria play a major role in producing useful bioactive compounds. HSV-1 infection causes 80% of oral lesions. A majority of the population is infected by at least one herpes subtype of HSV-1 before adulthood. The infection in some cases does not produce any symptoms. Drugs for HSV-1 are becoming suppressive. In the present study, isolation of endophytic bacteria was carried out from medicinal plant. A grown bacterium was identified and confirmed using molecular 16S rRNA sequencing followed by extraction of bioactive compounds using solvents. Twelve bioactive compounds were then investigated for in vitro cytotoxicity assay and in vitro antiviral assay. Chloroform extract was found to be effective in inhibiting the virus.
Objective: The aim of this study is to analyze the phytochemical constituents and the in vitro immunomodulatory potential of ethanol extract of Nigella sativa and Psoralea corylifolia seeds.Methods: Phytoconstituents in the ethanol extract of N. sativa and P. corylifolia seeds were analyzed using gas chromatography and mass spectrum and their immunomodulatory activity was investigated in vitro by cell proliferation assay with J774A.1 cell line.Results: Thirteen biochemical constituents have been identified with ethanol extract of N. sativa and nine from the P. corylifolia. The maximum relative activity of 138.77±0.44% cell proliferation was expressed at 25 μg/ml by N. sativa and 80.70±0.42% of proliferation by P. corylifolia at 25 μg/ml.
Conclusion:The results suggest that the ethanol extract of the plant seeds have stimulating activity on macrophage cells and could be useful for modulating immune functions.
Objective: The objective of this study was to evaluate the effective new phytocomponents from Calophyllum inophyllum ethanol leaf extract against breast cancer target protein of epidermal growth factor receptor (EGFR) using in silico docking studies.
Methods:The identification of compounds was done by gas chromatography-mass spectrometry (GC-MS) analysis. The in silico docking studies were carried out using Discovery Studio 4.0 software.
Results:The GC-MS analysis of ethanol leaf extract revealed the presence of eleven compounds. The docking analysis has exhibited moderate to potent inhibition with a range of dock score 3-55. 2H-Benzo(cd)pyrene-2,6(1,H)-dione, 3,5,7,10-tetrahydroxy-compound showed the dock score of 55.427.
Conclusion:The results revealed out that the compounds present in C. inophyllum can inhibit the EGFR protein. The plant possesses anticancer potential because of the various bioactive compounds presence which is mainly responsible for anticancer activity.
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