Macrophages are tissue-based phagocytes that play the central role in initiating defence mechanism of host immunity. Macrophage expresses inducible nitric oxide synthase (iNOS) that inhibit pathogen replication by releasing a variety of effector molecules which includes nitric oxide (NO). In the present study ethanol extract of Piper nigrum Linn. (white pepper) seed was investigated for its cytotoxicity and in vitro immunomodulatory properties using cell proliferation and NO determination assay with J774a-1, macrophage cell line. The ethanol seed extract was found to exhibit toxicity at higher concentrations of 50?g/ml and 100?g/ml. The seed preparation was observed to enhance both, the proliferation of macrophage cell with higher percentage proliferation of 29.24 at 12.5?g/ml and the production of NO with significant stimulation level of 47.74% at 12.5?g/ml compared to the control. Since the results show the modulator effect of Piper nigrum Linn. seed on macrophage cells, it could be considered to possess immunomodulatory potential.
Objective: The objective of this study was to evaluate the effective new phytocomponents from Calophyllum inophyllum ethanol leaf extract against breast cancer target protein of epidermal growth factor receptor (EGFR) using in silico docking studies.
Methods:The identification of compounds was done by gas chromatography-mass spectrometry (GC-MS) analysis. The in silico docking studies were carried out using Discovery Studio 4.0 software.
Results:The GC-MS analysis of ethanol leaf extract revealed the presence of eleven compounds. The docking analysis has exhibited moderate to potent inhibition with a range of dock score 3-55. 2H-Benzo(cd)pyrene-2,6(1,H)-dione, 3,5,7,10-tetrahydroxy-compound showed the dock score of 55.427.
Conclusion:The results revealed out that the compounds present in C. inophyllum can inhibit the EGFR protein. The plant possesses anticancer potential because of the various bioactive compounds presence which is mainly responsible for anticancer activity.
Traditional healers possess a rich knowledge on the use of medicinal plants for the treatment of various diseases. Enicostemma axillare (Lam). Raynal is one plant, used by healer to treat gastric cancer or ulceration. We assessed the phytochemical components of leaf extract for its antibacterial activity, to add value and provide an evidencebase for their traditional use. The antibacterial potential of the leaf extract was tested against Helicobacter pylori using agar well diffusion method. The minimum inhibitory concentration (MIC) of ethanol extract exhibited the (MIC) against H. pylori ranged from 200µg/ml -250µg/ml followed by chloroform extract. Gas chromatography and mass spectrum (GC-MS) analysis confirms the occurrence of different components in the ethanol and chloroform leaf extract of the studied species.
Objective: The aim of this study is to analyze the phytochemical constituents and the in vitro immunomodulatory potential of ethanol extract of Nigella sativa and Psoralea corylifolia seeds.Methods: Phytoconstituents in the ethanol extract of N. sativa and P. corylifolia seeds were analyzed using gas chromatography and mass spectrum and their immunomodulatory activity was investigated in vitro by cell proliferation assay with J774A.1 cell line.Results: Thirteen biochemical constituents have been identified with ethanol extract of N. sativa and nine from the P. corylifolia. The maximum relative activity of 138.77±0.44% cell proliferation was expressed at 25 μg/ml by N. sativa and 80.70±0.42% of proliferation by P. corylifolia at 25 μg/ml.
Conclusion:The results suggest that the ethanol extract of the plant seeds have stimulating activity on macrophage cells and could be useful for modulating immune functions.
Plants are widely used by all sections of the society either as folk medicines or as pharmaceutical preparation of modern
medicine. In vitro propagation of plants holds great promise for conservation and enhancement of valuable medicinal
plants. Cleome rutidosperma has been used in indian ayurvedic medicine for the treatment of a wide number of health
disorders. The present study deals with the influence of different plant growth regulators (PGR) including kinetin (Kin), 6-
Benzylaminopurine (BAP) and 2, 4-Dichlorophenoxyacetic acid (2,4-D) on the growth of plant and the identification and
comparison of bioactive constituents of wild and in situ propagated C. rutidosperma plant using Gas Chromatography -
Mass Spectrometry analysis (GC-MS). Nodal segments used as explants were cultured on Murashige and Skoog's medium
(MS) supplied with different concentrations of PGRs. Multiple shoot generation was achieved after 28 days of incubation.
The GC-MS analysis showed the presence of ten compounds of micropropagated and seven compounds of wild plants
were identified. The result concluded that various concentration of PGR had a significant role in in vitro regeneration of
plant and showed that the phytoconstituents of micropropagated plant is comparatively higher than that of wild plant.
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