In vitro Propagation and Comparative Phytochemical Analysis of Wild Plant and Micropropagated Cleome rutidosperma DC.

Abstract

Plants are widely used by all sections of the society either as folk medicines or as pharmaceutical preparation of modern medicine. In vitro propagation of plants holds great promise for conservation and enhancement of valuable medicinal plants. Cleome rutidosperma has been used in indian ayurvedic medicine for the treatment of a wide number of health disorders. The present study deals with the influence of different plant growth regulators (PGR) including kinetin (Kin), 6- Benzylaminopurine (BAP) and 2, 4-Dichlorophenoxyacetic acid (2,4-D) on the growth of plant and the identification and comparison of bioactive constituents of wild and in situ propagated C. rutidosperma plant using Gas Chromatography - Mass Spectrometry analysis (GC-MS). Nodal segments used as explants were cultured on Murashige and Skoog's medium (MS) supplied with different concentrations of PGRs. Multiple shoot generation was achieved after 28 days of incubation. The GC-MS analysis showed the presence of ten compounds of micropropagated and seven compounds of wild plants were identified. The result concluded that various concentration of PGR had a significant role in in vitro regeneration of plant and showed that the phytoconstituents of micropropagated plant is comparatively higher than that of wild plant.