Maize is a cereal crop which is an important source of food because it is the second source of carbohydrates after rice. Maize cultivation is faced with various plant diseases, that can reduce the quantity and quality of crop yield. Plant disease is one of the limiting factors for crop production and seed quality. One of the most harmful maize diseases is blight or midrib rot caused by the soil-borne fungus Rhizoctonia solani. The aim of the study was to obtain endophytic microorganisms in maize and to test the antagonistic ability of these fungi as biological control agents against R. solani. The study took place in January-September 2018 in the field and the Laboratory of Plant Pests and Diseases, Faculty of Agriculture, University of Pattimura. Endophytic fungi isolated from maize plants from the roots, stems and leaves. Samples were cleaned in running water and air-dried. Subsequently, the samples were sterilized using 70% alcohol and immersed in sodium hypochlorite solution for 2 minutes and rinsed with sterile water 3 times. The sterilized roots, stems and leaves of maize were peeled, cut to a size of ±5 mm, split and dried on sterile filter paper. Sterile samples were grown on PDA culture media at four points and incubated at room temperature. Visual identification was carried out with the aid of a microscope on fungi growing on the culture media. The results showed the presence of the endophytic fungi Trichoderma sp., Aspergillus sp., Penicillium sp., Diplodia sp. and Mucor sp. These fungal species showed different inhibitory powers, which included mechanisms for direct parasitizing of pathogens, producing antibiotics and competition for space and nutrients. Keywords: antagonism, corn, endophytic fungi, identification, pathogenic fungus, Rhizoctonia solani ABSTRAK Jagung (Zea mays L.) merupakan tanaman serelia yang termasuk bahan pangan penting karena merupakan sumber karbohidrat kedua setelah beras. Salah satu kendala dalam budidaya tanama jagung adalah penurunan produksi akibat serangan patogen penyebab penyakit. Untuk menekan perkembangan penyakit dilakukan pengendalian dengan menggunakan mikroorganisme endofitik asal akar, batang dan daun jagung sebagai agensia pengendalian hayati. Penelitian ini bertujuan mengisolasi dan mengidentifikasi mikroorganisme endofitik asal jagung secara morfologi, mendapatkan mikroorganisme endofitik yang memiliki kemampuan antagonis terhadap jamur Rhizoctonia solani penyebab penyakit busuk pelepah pada jagung. Penelitian berlangsung dalam 2 tahap, yaitu di lapangan dan di Laboratorium Hama dan Penyakit Tumbuhan Fakultas Pertanian, Universitas Pattimura. Sampel tanaman diperoleh dari beberapa kebun petani di wilayah Kota Ambon. Hasil penelitian yang diperoleh adalah bahwa jamur endofitik Trichoderma sp, Aspergillus sp, Penicillium sp, Diplodia sp dan Mucor sp. memiliki daya penghambatan yang berbeda-beda, yaitu 78.57% (Trichoderma sp), 65.86% (Penicillium sp), 43.29% (Aspergillus sp), 36.71 (Diplodia sp) dan 34.44% (Mucor sp). Kata kunci: antagonisme, identifikasi, jagung, jamur endofitik, jamurpatogenik, Rhizoctonia solani
Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.
Macroscopic mushrooms are mushrooms that have fruiting bodies. They live (heterotrophically). In addition, fungi are eukaryotic organisms, spores, do not have chlorophyll, and reproduce sexually and asexually. The aims of the research are (1) to find types of macroscopic fungi. (2) describe the dominant habitat types of the mushroom populations found. The research was carried out in West Leihitu District, namely the villages of Wakasihu and Larike from July to October 2022. The mushrooms were obtained by roaming methods on transect lines. Data collection included the habitat of the fungus, namely live wood, dead wood, soil, and leaf litter. Identification in the laboratory to determine the type of fungus. The data obtained were analyzed qualitatively and quantitatively. The results of the study obtained 16 species with 6 different species and 5 of the same species. In both villages, there are 8 species from the Basidiomycota division and 3 species from the Ascomycota division and consist of 8 families. The mushroom habitats found in both villages were: 7 species of dead wood, 3 species of soil, and 1 species of leaf litter. The frequency of the presence of the fungus is range between 7.8% to 10.2%.
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