Eksplorasi Dan Identifikasi Trichoderma Endofitik Pada Pisang

Taribuka, Johanna; Sumardiyono, Christanti; Widyastuti, Siti Muslimah; Wibowo, Arif

doi:10.23960/j.hptt.216115-123

Cited by 4 publications

(3 citation statements)

References 8 publications

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“…The conidiophore for three Trichoderma isolates is erect, branched, phialides are thick and short, the shape of the conidium is oval, the walls of the conidium are thick, the color of the conidia is green, and the hypha has something in common that is hyaline and insulated. Taribuka et al (2016), obtained the same morphological characteristics as the morphological characters of two Trichoderma isolates on bananas, namely Ksn and Psr 1. The difference is only between phialids and conidia sizes that are not far apart.…”

Section: Microscopic Characterizationmentioning

confidence: 72%

“…All the steps above need to be done for molecular identification with universal primers. As the study reported by Taribuka et al (2016), identified endophytic Trichoderma on banana plant roots molecularly using universal primers ITS 1 and ITS 2. Furthermore, the DNA sequences obtained were analyzed and compared with existing data in NCBI with the BLAST-N.…”

Section: Molecular Identification Of Trichoderma Speciesmentioning

confidence: 95%

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Characterization and identification of Trichoderma on shallots isolated from three elevation regions in West Sumatra, Indonesia

2023

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Abstract. Susila E, Maulina F, Emilda D. 2023. Characterization and identification of Trichoderma on shallots isolated from three elevation regions in West Sumatra, Indonesia. Biodiversitas 24: 2064-2071. Various microorganisms are found in the rhizosphere of plants. The species of Trichoderma exhibited many benefits to plants such as biological control of many crop diseases and their causing agents. This study aim was to explore, collect, and identify species of Trichoderma from shallot rhizosphere based on morphological characters and molecular techniques. The research was carried out from March to October 2021 at Biology Laboratories Agricultural State Polytechnic of Payakumbuh and Plant Protection Laboratory, Indonesian Tropical Fruits Research Institute (ITFRI) for morphological observation, and Molecular Laboratories, ITFRI for molecular identification. Samples were collected from Alahan Panjang (1700 m above sea level), Solok (400m asl) and Kambang (< 200 m asl) by using Stratified Random Sampling method. The isolates of Trichoderma species were cultured on Potato Dextose Agar (PDA). Three Trichoderma sp. from shallot rhizosphere were obtained and identified. Four specific primer pairs (T2A F-T2A R, T2 F-T2 R, T1 F-T1 R and Th1 F-Th1 R) were used for molecular identification. The amplification results of three isolates of Trichoderma spp. using four pairs of specific primers showed that the three isolates tested amplified only in the T. asperellum primer pair. The results showed that Trichoderma spp. origin of shallot rhizosphere from three locations (Alahan Panjang, Solok and Kambang) is identified as one species i.e. Trichoderma asperellum.

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Section: Microscopic Characterizationmentioning

confidence: 72%

Section: Molecular Identification Of Trichoderma Speciesmentioning

confidence: 95%

Characterization and identification of Trichoderma on shallots isolated from three elevation regions in West Sumatra, Indonesia

2023

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“…The clear zone formed around Trichoderma spp. colonies indicated the presence of chitinolytic activity, which was then calculated based on the [16] method.…”

Section: Physiological Characterization Of Trichoderma Sppmentioning

confidence: 99%

Trichoderma yunnanense and T. asperellum as potential biological agents for control of basal stem rot disease in oil palm

Budi

Giyanto

Tondok

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Basal Stem Rot (BSR) disease is a main constrain in oil palm cultivation. Ganoderma boninense is known as a causal agent of this disease. The intensity of BSR disease continues to increase in both the vegetative and generative phases of oil palm plantations and causes significant losses. Biological control is one of the BSR disease control techniques and is believed to be able to support sustainable oil palm cultivation. Trichoderma spp. is a group of fungal biological agents commonly used to control BSR disease. This study aims to obtain Trichoderma spp. isolates to be developed further as biocontrol agents for BSR disease. The research stages included isolation, morphology-based identification, antagonist potency testing, characterization, and identification with molecular technique. The results in this research obtained one isolate of Trichoderma strain TSU from oil palm plantation at Pematang Siantar Regency, and one isolate of Trichoderma strain TGLP from oil palm plantation at Musi Banyu Asin Regency, which inhibited the growth of G. boninense was 90.9 and 93.9% respectively through dual culture test. Inhibition tests by volatile compounds showed growth inhibition of G. boninense by both isolates were 55.2 and 70.6%. Both Trichoderma strains have the characteristics of producing chitinase, glucanase, and indole acetic acid. Furthermore, molecular identification showed that the Trichoderma strain TSU was similar to the Trichoderma yunnanense strain CBS121219 with an identity percentage of 99.11%, and Trichoderma TGLP as Trichoderma asperellum strain 1A4 with a percent identity of 99.65%. With their antagonistic ability and characteristics, the two Trichoderma spp. isolates have the potential to be further developed as biological agents for controlling BSR caused by G. boninense.

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In Vitro Evaluation of Trichoderma spp. against Sugarcane Eye Spot Disease (Bipolaris sp.)

Raharjo,

Dwi Indrawan,

Rahmadhini

2023

J. Ilmu. Pertan. Indones.

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Sugar cane (Saccharum officinarum L.) is the main ingredient in sugar production. Sugarcane is widely cultivated in warm and tropical areas and is widely used as a sweetener because it contains a lot of fructose and glucose. National sugar production shows a decline, the majority of which is caused by pathogens, especially the fungus Bipolaris sp. capable of producing up to 85% damage per Ha if no control is applied. Biological control using Trichoderma spp. can control this pathogen and is able to promote sugarcane growth. The purpose of this study was to determine the effectiveness of Trichoderma spp. in controlling Bipolaris sp. in vitro. The results showed isolates TD1, TD2, TD3 were able to inhibit Bipolaris sp. antagonistically and volatilely, the antagonist test showed a result of 86% (TD1) while the volatile test of 65% (TD2). Conclusion Trichoderma spp. can inhibit the growth of Bipolaris sp. on an in vitro scale. Keywords: BCA, Bipolaris sp. Trichoderma spp. ABSTRAK Tebu (Saccharum officinarum L.) merupakan bahan utama dalam pembuatan gula. Tebu banyak dibudidayakan didaerah hangat dan tropikal dan banyak digunakan sebagai pemanis dikarenakan mengandung banyak fruktosa dan glukosa. Produksi gula nasional menunjukan adanya penurunan yang mayoritas disebabkan oleh patogen terutama jamur Bipolaris sp. yang mampu menghasilkan kerusakan hingga 85% per Ha jika tidak diberlakukan pengendalian. Pengendalian biologis menggunakan Trichoderma spp. dapat mengendalikan patogen ini dan mampu mendorong pertumbuhan tebu. Tujuan penelitian ini ialah untuk mengetahui efektivitas Trichoderma spp. dalam mengendalikan Bipolaris sp. secara In vitro. Hasil menunjukkan isolat TD1, TD2, TD3 mampu menghambat Bipolaris sp. secara antagonis dan volatile, uji antagonis memperlihatkan TD1 memiliki hasil 86% sementara uji volatile TD2 memiliki hasil 65%. Kesimpulan Trichoderma spp. dapat menghambat pertumbuhan Bipolaris sp. dalam skala In vitro. Kata kunci: APH, Bipolaris sp. Trichoderma spp.

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Eksplorasi Dan Identifikasi Trichoderma Endofitik Pada Pisang

Cited by 4 publications

References 8 publications

Characterization and identification of Trichoderma on shallots isolated from three elevation regions in West Sumatra, Indonesia

Characterization and identification of Trichoderma on shallots isolated from three elevation regions in West Sumatra, Indonesia

Trichoderma yunnanense and T. asperellum as potential biological agents for control of basal stem rot disease in oil palm

In Vitro Evaluation of Trichoderma spp. against Sugarcane Eye Spot Disease (Bipolaris sp.)

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