Plant mutagenesis is rapidly coming of age in the aftermath of recent developments in high-resolution molecular and biochemical techniques. By combining the high variation of mutagenised populations with novel screening methods, traits that are almost impossible to identify by conventional breeding are now being developed and characterised at the molecular level. This paper provides a comprehensive overview of the various techniques and workflows available to researchers today in the field of molecular breeding, and how these tools complement the ones already used in traditional breeding. Both genetic (Targeting Induced Local Lesions in Genomes; TILLING) and phenotypic screens are evaluated. Finally, different ways of bridging the gap between genotype and phenotype are discussed.
Due to increased urbanisation, and climate change, there have been calls for a more sustainable management of stormwater. Blue-green measures have been recognised as a sustainable solution and a necessary complement to pipe-bound approaches. The aim of this study is to identify barriers and drivers in the implementation of bluegreen measures in a Swedish context, to increase the understanding of how they could be implemented in a more successful manner. The study is qualitative and based on semi-structured interviews. Through the lens of transition theory, barriers and drivers for blue-green measures were identified and they give an updated picture of Swedish urban stormwater management. Many factors encourage municipal actors to implement blue-green solutions, such as increased need for recreation, protection of biodiversity and climate change. Identified barriers are found within the municipal stormwater management it-self, but can also be found outside the storm water management structure, such as lack of knowledge among politicians, officials, exploiters and civilians, fragmented roles and responsibilities in general, as well as uncertainty of the effects and cost of new alternatives. The study has three main findings; Several barriers were mentioned by most of the interviewees clearly show that a wide range of changes are needed to alter the current stormwater management regime; Niche innovations are often put forward as a way to enhance socio-technical transition, but this study is that such an approach is oversimplified instead elaborated suggestions for an alteration of urban stormwater management is given, both with top-down and bottom-up perspective. For the success of blue-green solutions, educational efforts are important at different levels in the planning, building and maintenance process of blue-green solutions. Therefore, employees must have a good general knowledge of both blue and green issues as well as having contacts in the different sectors of the municipality. To conclude we argue that a transition can not only be induced by pilot projects but requires change in legal structures as well as altered financing models for blue-green solutions. Moreover, the ongoing, but slow, change should therefor probably be interpreted as a shift to a new regime, but rather an evolutionary transition where new approaches are combined with traditional, pipe-bound solutions.
Politicians and policy-makers, as well as modellers, often nurses an expectation that model derived results is an objective source of information that can be used to support decisions. However, several prerequisites have to be dealt with in order to ensure that models can be used as legitimate and efficient tools in water resource management. Based on empirical material from recent studies on the use of models in stakeholder dialogues, mainly focusing on catchment nutrient transport, two central problems are identified: (a) Models are laden with choices and thus depend on assumptions and priorities of modellers. (b) There are several factors that influence ability and willingness of stakeholders (as information recovers) to criticize or accept results of the modelling exercise. Recognized factors likely to influence stakeholders' acceptance of model derived results include issues at stake, stakeholders' ability to criticize model derived information, and their trust in the institutions that have developed or applied the used models. Identified prerequisites for successful use of models in integrated water resource management include: consideration of user relevance, awareness of and preparedness to handle constraints linked to communication of modelbased results, transparency of used models and data and of involved uncertainties, mutual respect between experts and stakeholders and between involved stakeholder groups, a robust institutional network, and sufficient time for dialogues. Development and use of strategies for participatory modelling, based on a continuous dialogue between experts and stakeholders is recommended as a way to facilitate that the prerequisites for a successful use of models in water resource management are fulfilled.
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