Mutagenesis as a Tool in Plant Genetics, Functional Genomics, and Breeding

Sikora, Per; Chawade, Aakash; Larsson, Mikael; Olsson, Johanna Alkan; Olsson, Olof

doi:10.1155/2011/314829

Cited by 206 publications

(158 citation statements)

References 76 publications

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“…The mutant lines exhibiting significantly higher grain yield or higher tolerance to nutrient deficiency or abiotic stresses or other traits can be used for rice breeding by conventional backcrossing along with molecular marker-assisted selection (Jiang and Ramachandran, 2010;Sikora et al, 2011). EMS induced rice mutants were used to investigate the complex mechanism involved in water and salt stress tolerance (Huang et al, 2009;Zhou et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Yugandhar¹,

Basava²,

Subrahmanyam³

et al. 2017

Aust J Crop Sci

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Mutants are powerful genetic resources in plant breeding and functional genomics studies. Sixty seven stable ethyl methane sulphonate (EMS) induced rice mutants and the wild type parent Nagina 22 (N22) were characterized for plant height, tiller number, panicle number and grain yield under normal, low P field and alternate wet and dry (AWD) conditions in the same season. They were also genotyped with 44 SSR markers and four Pup1 (Phosphorus uptake1) gene specific markers. Genetic diversity was analysed by combining phenotype and marker data using Ward-MLM method. Single marker analysis showed significant association of four markers RM19696, RM263, RM3688 and RM1942 with grain yield in all three conditions. K-1, a Pup1 gene specific marker was significantly associated with tiller number only under low P conditions. The average dissimilarity between mutants was 0.86 and cophenetic correlation coefficient was 0.74. Six mutants were selected as gain-of-function mutants as they showed significantly higher grain yield in all three conditions, compared with N22. The selected mutants are an important resource for gene discovery for enhanced tolerance to low P and water stress conditions and associated markers can be useful in marker assisted selection.

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Section: Introductionmentioning

confidence: 99%

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Yugandhar¹,

Basava²,

Subrahmanyam³

et al. 2017

Aust J Crop Sci

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“…To achieve desired objectives through conventional breeding approaches is highly depended on adopted techniques. Raising of induced variant/mutants populations with proper screening techniques is useful to identify the desirable traits among the mutant populations and to evaluate their adaptability (Sikora et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

NIAB-2008: A New High Yielding and Long Staple Cotton Mutant Developed through Pollen Irradiation Technique

Haider¹,

Aslam²

2016

IJAB

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To cite this paper: Haidar, S. and M. AbstractThis paper describes about development of cotton mutant NIAB-2008, developed through pollen irradiation technique with improved yield and fibre quality. A local variety NIAB-78 was crossed with exotic line REBA-288 using irradiated pollen @10 Gray (Gy) of gamma rays before cross pollination. The purpose of the study was to create new genetic variability and select the desirable new cotton mutants. After irradiation and hybridization different generations were raised to evaluate the effect of irradiation treatment and induction of mutations. Significant variations from control/parents were observed in evaluated generations. From M1 seed, the M2 population was raised and different desirable mutants were selected. The selected mutants were evaluated in mutated generations (M3-M6) for different yield contributing traits and yield potential along with fibre quality and variations from parents were observed. From selected mutants, an elite mutant i.e., M-7/09, later named as NIAB-2008 was finally selected. It was evaluated for seed cotton yield, adaptability, resistance/tolerance to diseases, and fibre quality in different trials. It produced 32.9% higher seed cotton yield compared to standard CIM-496 at local NIAB trials. It produced 32.7% and 32.2% higher seed cotton yield compared to standard in provincial coordinated cotton trials (PCCT) and in national coordinated varietal trials (NCVT) respectively. The mutant NIAB-2008 has desirable fibre quality traits i.e., ginning out turn (GOT) 37.99%, fibre length 31.16 mm (long staple category), fibre fineness 4.74 µg/inch, uniformity index 83.5%, fibre maturity 80.5% and fibre strength 92.2 thousand pounds per square inch (TPPSI). Its distinguish characteristics are early maturity, high yielding, short stature, good boll bearing and better tolerance to cotton leaf curl virus-Burewala strain (CLCuV-B) disease. From these results it is concluded that low dose pollen irradiation technique has effectively stimulate/increase the different yield and yield contributing traits, fibre quality and tolerance to diseases in cotton and this technique is proved economical.

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“…Because such application of physical mutagens required specialized equipment, chemical mutagens were introduced later. Chemical mutagens are used widely because they are easier to handle and increase mutation frequency (Sikora et al, 2011). It is a coherent tool used in mutation breeding program for creating new alleles (Laskar and Khan, 2014).…”

Section: Introductionmentioning

confidence: 99%

Mutagenic effectiveness and efficiency of Gamma rays and EMS in Lablab purpureus (L.) Sweet var. typicus

Monica¹,

Seetharaman²

2016

Int. J. Adv. Multidiscip. Res.

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Mutation has been successfully employed in breeding of several food crop varieties, ornamentals and export crops. The aim of this study was undertaken to assess the effectiveness and efficiency of EMS and gamma rays on Lablab purpureus (L.) Sweet var. typicus cv. CO(Gb)14. Genetically pure, uniform and dry seeds were treated with different doses/ concentrations of gamma rays (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 KR) and EMS (5mM, 10mM, 15mM, 20mM, 25mM, 30mM, 35mM, 40mM, 45mM and 50mM). Mutagenic effectiveness and efficiency of gamma rays and EMS was calculated on the biological damage (lethality, injury and pollen sterility) in the M 1 generation and both chlorophyll and morphological mutations observed in the M 2 generation. The results indicated that, mutagenic effectiveness and efficiency decreased with increase in mutagenic treatments.

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Mutagenesis as a Tool in Plant Genetics, Functional Genomics, and Breeding

Cited by 206 publications

References 76 publications

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

NIAB-2008: A New High Yielding and Long Staple Cotton Mutant Developed through Pollen Irradiation Technique

Mutagenic effectiveness and efficiency of Gamma rays and EMS in Lablab purpureus (L.) Sweet var. typicus

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