Lactic acid bacteria were selected for their inhibitory activity against Clostridium tyrobutyricum under conditions that eliminate the effects of lactic acid and hydrogen peroxide. Four strains were isolated belonging to the species Lactococcus lactis ssp. lactis. The sensitivity of the inhibitory substances to pronase and trypsine indicates that they are proteins or peptides different from nisin. Their resistance to phospholipase D indicates that they are also different from lactostrepcin. The inhibitory substances are produced during the exponential phase of growth. Their activity is bactericidal and directed toward some strains of Clostridium tyrobutyricum, Lactobacillus helveticus, and Streptococcus thermophilus, but strains used as dairy starters, Lactobacillus lactis, Streptococcus thermophilus, and Propionibacterium shermanii, are not all affected by the inhibition.
Erwinia chrysanthemi (biovars 5 and 6) was isolated from unusual symptoms on witloof chicory, both in the field and in hydroponic culture, in Brittany in 1989 and 1990. Symptoms included a greyish‐brown soft rot on the lower part of the root and the destruction of the cortical tissues. The cribrovascular and the medullary part of the bottom of the root sometimes became slimy. A few cases of vascular transmission were observed, which resulted in a red coloration of the infected vessels and a soft rot of the leaves.
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