Inhibition of Clostridium tyrobutyricum by Bacteriocin-Like Substances Produced by Lactic Acid Bacteria

Abstract

Lactic acid bacteria were selected for their inhibitory activity against Clostridium tyrobutyricum under conditions that eliminate the effects of lactic acid and hydrogen peroxide. Four strains were isolated belonging to the species Lactococcus lactis ssp. lactis. The sensitivity of the inhibitory substances to pronase and trypsine indicates that they are proteins or peptides different from nisin. Their resistance to phospholipase D indicates that they are also different from lactostrepcin. The inhibitory substances are produced during the exponential phase of growth. Their activity is bactericidal and directed toward some strains of Clostridium tyrobutyricum, Lactobacillus helveticus, and Streptococcus thermophilus, but strains used as dairy starters, Lactobacillus lactis, Streptococcus thermophilus, and Propionibacterium shermanii, are not all affected by the inhibition.

IMPROVING PHYSICO-CHEMICAL CHARACTERISTIC AND PALATABILITY OF KING GRASS (Pennisetum hybrid) SILAGE BY INOCULATION OF Lactobacillus plantarum - Saccharomyces cerevisiae CONSORTIA AND ADDITION OF RICE BRAN

“…Both of microbes in inoculants were previously reported that those had ability to produce antibacterial substances. In term, L. plantarum secretes bacteriocins substances (Thuault et al, 1991;Gollop et al, 2005;Valan-Arasu et al, 2013) and S. cerevisiae generates oxylipins (Strauss et al, 2005) that those affect Clostridia and fungi inhibition.…”

The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis: location of the thioether bridges

“…wide range of Gram-positive bacteria [3,4]. In this respect, its potential use as an agent to prevent late blowing of cheese due to Clostridium tyrobutyricum has been proposed [4].…”

“…In this respect, its potential use as an agent to prevent late blowing of cheese due to Clostridium tyrobutyricum has been proposed [4]. The structural gene of lacticin 481 has been sequenced together with two adjacent genes, believed to be involved in lacticin 481 biosynthesis [5][6][7].…”

Bacteriocin Detection from Whole Bacteria by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

“…These values were repeatedly obtained with a standard deviation of 1 and are consistent with the masses of the molecular ion [MϩH] ϩ and the adduct ions [MϩNa] ϩ and [MϩK] ϩ of lacticin 481. To verify that these peaks were indeed due to lacticin 481, we compared the following two pairs of strains: L. lactis C2102 and C2109 (Table 1), both of which were obtained from the wild-type lacticin 481 producer L. lactis ADRIA85LO30 (20,28) and which differ by the presence (C2102) or the absence (C2109) of the lacticin 481 operon (5); and L. lactis IL-1403(pEB94) and IL1403(pBS-pIL253), which carry the lacticin 481 operon on a recombinant plasmid and only its vector portion, respectively (21) ( Table 1). The expected peak cluster appeared in the spectra for the two lacticin 481-producing strains but not in the spectra for their nonproducing counterparts (data not shown) ( Table 2).…”

“…Lacticin 481 is a 2,901-Da lantibiotic (Fig. 1B) produced by some L. lactis strains (Table 1) (18,19,20,28). When whole bacteria grown on a plate were analyzed by MALDI-TOF-MS, a peak cluster covering the expected m/z value (m/z is the mass/charge ratio, where z is usually 1) was observed in the spectra from three different wild-type strains that produce lacticin 481, strains ADRIA85LO30 ( Fig.…”