Temperate pasture species constitute a source of protein for dairy cattle. On the other hand, from an environmental perspective, their high N content can increase N excretion and nitrogenous gas emissions by livestock. This work explores the effect of energy supplementation on N use efficiency (NUE) and nitrogenous gas emissions from the excreta of dairy cows grazing a pasture of oat and ryegrass. The study was divided into two experiments: an evaluation of NUE in grazing dairy cows, and an evaluation of N-NH3 and N-N2O volatilizations from dairy cow excreta. In the first experiment, 12 lactating Holstein × Jersey F1 cows were allocated to a double 3 × 3 Latin square (three experimental periods of 17 days each) and subjected to three treatments: cows without supplementation (WS), cows supplemented at 4.2 kg DM of corn silage (CS) per day, and cows supplemented at 3.6 kg DM of ground corn (GC) per day. In the second experiment, samples of excreta were collected from the cows distributed among the treatments. Aliquots of dung and urine of each treatment plus one blank (control – no excreta) were allotted to a randomized block design to evaluate N-NH3 and N-N2O volatilization. Measurements were performed until day 25 for N-NH3 and until day 94 for N-N2O. Dietary N content in the supplemented cows was reduced by 20% (P < 0.001) compared with WS cows, regardless of the supplement. Corn silage cows had lower N intake (P < 0.001) than WS and GC cows (366 v. 426 g/day, respectively). Ground corn supplementation allowed cows to partition more N towards milk protein compared with the average milk protein of WS cows or those supplemented with corn silage (117 v. 108 g/day, respectively; P < 0.01). Thus, even though they were in different forms, both supplements were able to increase (P < 0.01) NUE from 27% in WS cows to 32% in supplemented cows. Supplementation was also effective in reducing N excretion (761 v. 694 g/kg of Nintake; P < 0.001), N-NH3 emission (478 v. 374 g/kg of Nmilk; P < 0.01) and N-N2O emission (11 v. 8 g/kg of Nmilk; P < 0.001). Corn silage and ground corn can be strategically used as feed supplements to improve NUE, and they have the potential to mitigate N-NH3 and N-N2O emissions from the excreta of dairy cows grazing high-protein pastures.

Annual temperate grasses go through abrupt changes in morphological composition during the growing season, which can affect ingestive behavior of grazing cattle. However, few studies have examined the effects of gradual structural changes in annual temperate pastures on the herbage intake rate during the growing season. We hypothesized that lenient grazing (removal of 40% of the initial pre‐grazing height) maintains short‐term intake rate (STIR) of grazing cattle over the entire growing season, even with decreased leaf/stem ratios. The studied pastures contained Avena strigosa, Lolium multiflorum and a mixture of the two species. Short‐term intake rate was measured using the double weighing technique, evaluating both bite rate and bite mass. The percentage of stem + pseudostem and leaf lamina lengths in the herbage mass were measured. Relationships between sward structure and animal ingestive behavior were also examined. As the pasture growing season progressed, the STIR of heifers grazing annual temperate grass swards was affected more by the time required to take a bite than by bite mass. Moreover, the sward structure also changed after each grazing cycle, reducing the pre‐grazing leaf/stem ratio. However, reductions in the pre‐grazing leaf lamina mass (up to 37% of the sward herbage mass) and/or in the leaf lamina length (up to 52% of the extended tiller height) did not affect the STIR of grazing heifers, since the animals maintained their bite mass and time per bite. In summary, with a grazing down target of 40% of the pre‐grazing height, reductions in STIR of heifers grazing annual temperate grasses occurred when the pre‐grazing leaf lamina percentage was <37% of the sward herbage mass and/or <52% of the extended tiller height. These results suggested that to avoid STIR reductions during the last grazing cycles, managements to stimulate tillering and delay stem + pseudostem elongation are necessary and should be investigated.

The inclusion of grazed pasture in dairy feeding systems based on a total mixed ration (TMR) reduces feed costs, benefits herd health, and reduces environmental impact. The present study aimed to evaluate the effect of ryegrass pasture combined with a partial TMR on enteric methane emissions, dry matter intake (DMI), and performance of dairy cows from mid to late lactation. The experimental treatments included 100% TMR (control), partial TMR + 6h of continuous grazing (0900-1500 h), and partial TMR + 6h of grazing that was divided into 2 periods of 3h each that took place after milking (0900-1200 h; 1530-1830 h). Twelve F1 cows (Holstein × Jersey; 132±44 DIM) were divided into 6 lots and distributed in a 3×3 Latin square design with 3 periods of 21 d (15 d of adaptation and 6 d of evaluation). Ryegrass (Lolium multiflorum Lam.) pasture was used, and the TMR was composed of 80% corn silage, 18% soybean meal, and 2% mineral and vitamin mixture, based on dry matter. The same mixture was used for cows with access to pasture. The total DMI, milk production, and 4% fat-corrected milk were similar for all cows; however, the pasture DMI (7.4 vs. 6.0kg/d) and grazing period (+ 40 min/d) were higher in cows that had access to pasture for 2 periods of 3h compared with those that grazed for a continuous 6-h period. Methane emission was higher (656 vs. 547g/d) in confined cows than in those that received partial TMR + pasture. The inclusion of annual ryegrass pasture in the diet of dairy cows maintained animal performance and reduced enteric methane emissions. The percentage of grazed forage in the cows' diet increased when access to pasture was provided in 2 periods after the morning and afternoon milking.

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The amount of digesta flowing to the duodenum is a relevant measurement for the evaluation of nutrient supply to ruminants, which is usually estimated in animals fitted with a duodenal T-type cannula using internal or external markers. This study evaluated acid detergent fiber (ADF) compared with external (C32n-alkane) and internal [sulfuric acid lignin (ADL) and n-alkanes C31 and C33] markers for estimating duodenal flow and(or) ruminal digestibility of dry matter (DM) in cattle and sheep. In the first assay, 4 duodenally cannulated Holstein steers housed in metabolism cages, dosed with C32n-alkane, and fed Avena strigosa plus concentrate and increasing levels of tannin extract to reduce ruminal digestibility, were used in a Latin square design. The mobile-bag technique was used to measure the intestinal disappearance of ADL and ADF from forage (Avena strigosa, Pennisetum purpureum, Cynodon dactylon, and Medicago sativa) and concentrate (corn grain, soybean meal, and sunflower meal) samples that were previously incubated in the rumen of additional fistulated steer for 12, 24, 36, or 48 h. The ADF concentration in residues recovered in the feces was strongly related to the ADF concentration in residues at the duodenum (R(2)=0.93, standard deviation=30.0, n=901). This relationship showed a lower precision for ADL fraction (R(2)=0.88, standard deviation=12.6, n=590). In a second assay, duodenal flow and ruminal DM digestibility were calculated from the duodenal and fecal concentration of either marker. We observed a significant effect of marker type on ruminal DM digestibility values, and the effect of tannin treatments was observed only when ADF or ADL was used as the marker. The lowest residual error was obtained for ADF. Ruminal DM digestibility was, on average, higher for C31 and C(33)n-alkanes, and the use of dosed C(32)n-alkane resulted in a negative value. In the third assay, a data set of 235 individual observations was compiled from digestibility trials to compare ADF and ADL as markers for estimating duodenal digesta flow in wethers (n=204) and cattle (n=31). We observed a strong relationship between markers (R(2)=0.84 in sheep and R(2)=0.88 in cattle), but variance analysis within trials indicated that ADF was more precise than ADL. In conclusion, in digestibility trials in which fecal output was measured and spot samples of the duodenal digesta were obtained, duodenal flow and ruminal digestibility of the DM may be estimated from the relationship between the ADF concentration in feces and that in the duodenal digesta of ruminants.

The excretion of urinary nitrogen (N), one of the most important environmental contaminants from livestock systems, is highly correlated with milk urea N content. The objective of this research was to evaluate the use of different types of corn supplementation on milk urea N in grazing dairy cows. Twelve Holstein × Jersey lactating dairy cows were divided into six uniform groups according to milk production, lactation stage and live weight. Treatments were compared according to a 3 × 3 replicated Latin square experimental design, with three periods of seventeen days (twelve days to adaptation and five to measurements). The experimental treatments were exclusively grazing (G); grazing + supplementation with 4.2 kg DM of corn silage (CS) and grazing + supplementation with 3.2 kg DM of ground corn (GC). The pasture used was annual ryegrass (Lolium multiflorum L.) and white oats (Avena sativa L.). The milk protein production increased 65 g/day in the GC treatment group compared to the G and CS groups. The supplemented dairy cows showed lower milk urea N (-2.8 mg/dL) than unsupplemented cows, but the N utilization efficiency (g N output in milk/ g N intake) did not change between treatments (average = 0.26). Additionally, there was a relationship between milk and plasma urea nitrogen concentrations (R2 = 0.64). In conclusion, for dairy cows grazing annual temperate pastures, corn ground supplementation increased milk protein production and reduced the excretion of milk urea N, whereas corn silage reduced the excretion of milk urea N without affecting milk protein production.

In the context of the science of animal welfare, the rearing system of sows is an especially significant subject, as the great majority of these animals is kept in isolation. The purpose of this paper is to present the main points connected with the rearing system of gestating sows, focusing on the importance of aspects related to the social and physical environment provided to this kind of animal. The isolation in cages undoubtedly has negative impacts on fundamental requisites for animal welfare. On the other hand, group housing has the disadvantage of causing aggressiveness in such animals. Research information shows that bioclimatic, behavioral and physical aspects of the environment are important in defining the welfare of gestating sows. Furthermore, it is important to devise rearing system alternatives which combine group housing and techniques to reduce aggressiveness, such as environmental enrichment, by using elements like straw or other types of bedding.

Increasing concerns regarding environmental impacts of animal production require a better understanding of the factors that influence nitrogen (N) excretion by animals and the processes that influence N volatilization into ammonia (NH3) and nitrous oxide (N2O) from manure. The objective of this study was to evaluate the influence of diet characteristics and climatic factors on manure composition, as well as the resulting NH3 and N2O emissions in the barn and during storage of a straw-based litter system. Two groups of three dairy cows were housed in mechanically ventilated rooms and fed with a grass-based diet (GD) or a total mixed diet (MD). The resulting solid manures were stored in ventilated tunnels. The experiment was conducted in autumn (AUT) and spring (SPR). NH3 and N2O emissions were recorded continuously (28 days in the barn, 85 days for storage). NH3–N emissions in the barn were higher for GD-AUT than for MD-AUT, which was consistent with the larger and unbalanced amount of crude and degradable protein in GD, and corroborated by higher milk urea N contents. More than 80% of the NH3–N volatilization occurred during the first week of manure storage, when the temperature of the manure heap peaked. N2O–N emissions were negligible in the barn. During storage, N2O–N emissions peaked immediately after the first week. Higher N2O–N emissions were related to higher rainfall, which may have increased the moisture content and decreased the temperature of the manure heap, thus generating the conditions necessary for nitrification and denitrification processes.