RESUMO: "Atividade antimicrobiana e perfi l fi toquímico das raízes de Lippia alba (Mill.) N.E. Brown". Lippia alba (Mill.) N.E. Brown (Verbenaceae) é geralmente usada na medicina popular brasileira para o tratamento de doenças gástricas, febre, asma e como tranqüilizante. Este trabalho avaliou a atividade antimicrobiana dos extratos acetato de etila, metanol e aquoso das raízes de L. alba usando métodos de difusão em poços e o perfi l fi toquímico. Os resultados obtidos mostraram que os extratos acetato de etila e metanol apresentaram atividade antimicrobiana contra Staphylococcus aureus (ATCC 6538P), Staphylococcus aureus (ATCC 6538) e Klebsiella pneumonia (ATCC 10031). Terpenóides, fenilpropanóides e açúcares foram detectados na análise fi toquímica.Unitermos: Lippia alba, Verbenaceae, atividade antimicrobiana, análise fi toquímica.ABSTRACT: Lippia alba (Mill.) N.E. Brown (Verbenaceae) is commonly used in the Brazilian folk medicine to the treatment of gastric illnesses, diarrhea, fever, asthma, and as a tranquilizer. This work evaluated the antimicrobial activity of ethyl acetate, methanol and aqueous extracts from the roots of the L. alba using plates-holes diffusion assay and the phytochemical profi le. The results obtained showed that the ethyl acetate and methanol extracts presented antimicrobial activity against Staphylococcus aureus (ATCC 6538P), Staphylococcus aureus (ATCC 6538) and Klebsiella pneumonia (ATCC 10031). Terpenoids, phenylpropanoids and sugars were detected in the phytochemical analysis.
Actinomycetes are known to produce numerous secondary bioactive metabolites of pharmaceutical interest. The purpose of this study was to isolate, characterize, and investigate the antibacterial, antifungal, and anticancer activities of metabolites produced by Actinobacteria isolated from the rhizosphere of Paullinia cupana. The Actinobacteria was identified as Streptomyces hygroscopicus ACTMS-9H. Based on a bioguided study, the methanolic biomass extract obtained from submerged cultivation had the most potent antibacterial, antifungal, and cytotoxic activities. This extract was partitioned with n-hexane, ethyl acetate, and 2-butanol. Elaiophylin was isolated from the methanolic biomass extract, and its molecular formula was determined (CHO) based on H andC NMR, IR and MS analyses. The 2-butanol phase was fractionated into four fractions (EB1, EB2A, EB2B, and EB3M). Chemical prospecting indicated the presence of alkaloids, saponins, and reducing sugars in the methanolic extract and 2-butanol phase. The elaiophylin displayed anticancer activity in HEp-2 and HL-60 cells with an IC of 1 μg/mL. The EB1 fraction was selectively toxic to HL-60 cells with IC of 9 ng/mL. Bioautography showed that the EB1 fraction contained an alkaloid with antibacterial and antifungal activities (MIC values ≤1.9 and <3.9 μg/mL, respectively). In conclusion, the EB1 fraction and elaiophylin of S. hygroscopicus have potent antimicrobial, antifungal, and anticancer activities.
An experiment was conducted on female Swiss albino mice to determine the acute toxicity of Xylopia frutescens seeds including the highest dose that killed any treated animal as well as the dose capable of killing all animals. A phytochemical screening of the secondary metabolites of the seeds was also conducted to determine the possible constituents that may cause toxicity in mice. At 250 mg/kg, the mice were agitated with an increase in respiration and appeared pale and sleepy. Mice treated with 600 mg extract/kg showed an increase in respiration, pale colour, sleepiness, aggressive behaviour and photophobia. Mice at 900 mg extract/kg were highly agitated with conjunctive irritation and were prostrate. The 1125 mg/kg dose killed all the mice within 24 hours. Monoterpenoids, sesquiterpenoids, diterpenoids and triterpenoids were found in the ethanolic extracts of X. frutescens seeds.
O presente artigo explana a relação entre os ambientes físicos corporativos e a saúde mental dos seus funcionários no Brasil no momento atual. Tendo sido considerada a depressão como a doença do século XXI e o ambiente laboral um dos lugares onde as pessoas passam a maior parte do seu tempo, foi questionado se, além das pressões por rendimento e as grandes demandas de trabalho, o ambiente físico interno das empresas exerceria alguma interferência sobre o estado psicológico dos colaboradores. O objetivo deste trabalho foi pesquisar o poder de influência destes ambientes sobre o bem-estar dos seus funcionários e buscar estratégias de projeto que afastassem doenças psicológicas relacionadas ao trabalho, como a Síndrome de Burnout. A metodologia está alicerçada sobre pesquisas bibliográficas em livros, artigos e sites de internet. Os resultados encontrados apresentam relação direta entre o ambiente físico de trabalho e a saúde mental das pessoas e que estratégias de projeto baseadas nos conceitos do design biofílico são capazes de evitar ou amenizar doenças mentais. Concluiu-se que esta pesquisa pode servir como referência inicial para o desenvolvimento de novos projetos de escritórios saudáveis, assim como de apoio para a melhoria de ambientes corporativos já construídos.
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