Antimicrobial activity and phytochemical profile from the roots of Lippia alba (Mill.) N.E. Brown

Filho, José G. Sena; Melo, João Gabriel Bandeira Silva de; Saraiva, Antonio Marcos; Gonçalves, Ademário M.; Psiottano, Maria Nelly Caetano; Xavier, Haroudo Sátiro

doi:10.1590/s0102-695x2006000400012

Cited by 25 publications

(17 citation statements)

References 12 publications

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“…Alíquotas de 10 μL foram analisadas por cromatografia em camada delgada (placas de gel de sílica Merck -Alemanha, art.105554) para análise da presença de grupos de metabólitos, empregando-se diversas fases móveis e reveladores adequados (Harborne, 1984;Wagner & Bladt, 1996;Sena Filho et al, 2006).…”

Section: Perfil Fitoquímico Da Própolis Através Da Ccdunclassified

Variabilidade sazonal dos constituintes da própolis vermelha e bioatividade em Artermia salina

Nunes¹,

Galindo²,

Deus³

et al. 2009

Rev. bras. farmacogn.

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RESUMO:A própolis é uma substância resinosa coletada pelas abelhas de diversas partes das plantas. Sua composição depende da época, vegetação e local de coleta. Apresenta diversas atividades biológicas como antimicrobiana, antioxidante, antitumoral, dentre outras. Foi realizado estudo da variabilidade sazonal, nos meses de fevereiro, junho e outubro de 2006, dos constituintes voláteis da própolis vermelha de Pernambuco através da extração por headspace dinâmico e identificação por cromatografia gasosa acoplada com espectrometria de massas (CG-EM). Foram identificados 34 constituintes voláteis, sendo monoterpenos e monoterpenóides, sesquiterpenos e sesquiterpenóides, fenilpropanóides, aldeídos, cetonas e η-alcanos. Os constituintes majoritários foram o trans-anetol, α-copaeno e o metil cis-isoeugenol. Também foi realizado o perfil fitoquímico por cromatografia em camada delgada (CCD), através da qual os constituintes fenólicos foram identificados como majoritários. Com o extrato bruto metanólico da própolis, realizou-se o ensaio de letalidade em Artemia salina, que demonstrou DL 50 de 18,9 µg/mL, sugerindo uma possível atividade antitumoral. Unitermos: Própolis vermelha, constituintes voláteis, Artemia salina.ABSTRACT: "Seasonal variability of red propolis constituents and brine shrimp bioassay". Propolis is a resinous hive product collected by honeybees from plant sources. The composition of the propolis depends on the season, the vegetation, and the area of collection. It presents a diverse biological activities such as antimicrobial, antioxidant and antitumoral. The seasonal variability of the red propolis constituents from Pernambuco State were accomplished in the months of February, June and October of 2006. The volatile red propolis constituients were captured by the dynamic headspace technique and analyzed by GC-MS. In the analysis of the samples, 34 constituents were identified as the mono and sesquiterpens, phenylpropanoids, aldehydes, cetons and n-alcanes, presenting as majority constituents the trans-anethol, α-copaene and methyl cis-isoeugenol. The phytochemistry profile was performed by thin layer chromatography (TLC). Through this technique the majority constituents were identified as phenols. The Brine shrimp bioassay was used to evaluate of the red propolis methanolic extract, which demonstrated a DL 50 of 18.9 µg/mL and an activity antitumoral was possibly suggested.

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Section: Perfil Fitoquímico Da Própolis Através Da Ccdunclassified

Variabilidade sazonal dos constituintes da própolis vermelha e bioatividade em Artermia salina

Nunes¹,

Galindo²,

Deus³

et al. 2009

Rev. bras. farmacogn.

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“…Chemical tests to detect the main classes of secondary metabolites were carried out using classical specifi c colour reactions (Mattos, 1988;Steinegger & Hansel, 1992;Barbosa, 2001;Sena Filho et al, 2006;Migliato et al, 2007) and by spraying colour reagent on thin layer chromatograms (Wagner & Bladt, 1996).…”

Section: Phytochemical Analysismentioning

confidence: 99%

Arrabidaea chica (HBK) Verlot: phytochemical approach, antifungal and trypanocidal activities

Barbosa¹,

Pinto²,

Quignard³

et al. 2008

Rev. bras. farmacogn.

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RESUMO: "Arrabidaea chica (HBK) Verlot: abordagem fi toquímica, atividades tripanocida e antifúngica". Arrabidaea chica (HBK.) Verlot (Bignoniaceae) popularmente, "Pariri", é um arbusto escandente, distribuído do sul do México até a Guiana e Brasil central e é tradicionalmente indicado para tratar sintomas de infl amações e afecções da pele. Seu extrato etanólico foi quimicamente investigado e testado contra leveduras e fungos dermatófi tos. A atividade tripanocida do mesmo extrato foi também avaliada. Este trabalho reporta o isolamento de três fl avonóides, a inibição total do crescimento de Trichophyton mentagrophytes e um signifi cante efeito tripanocida do extrato etanólico e de suas frações. Não foi detectada qualquer toxicidade aguda relevante, mesmo a uma dose de 1000 mg/kg. Unitermos: Arrabidaea chica, Bignoniaceae, fl avonóides, Trichophyton mentagrophytes, atividade antifúngica, Trypanosoma cruzi, efeito tripanocida. ABSTRACT:Arrabidaea chica (HBK.) Verlot (Bignoniaceae) vernacular name "Pariri", is a climbing shrub, widespread from South Mexico to Guyana and central Brazil and is traditionally indicated to treat symptoms of infl ammations and skin affections. Its ethanol extract was chemically investigated and tested against yeasts and dermatophytic fungi. The trypanocidal activity of the same extract was also evaluated. This work reports the isolation of three fl avonoids, the total growth inhibition of Trichophyton mentagrophytes and a signifi cant trypanocidal effect of the ethanol extract and its fractions. No relevant acute toxicity was detected even at a dose of 1000 mg/kg.

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“…For the phytochemical analysis we performed phytochemical screening according to Matos [14] and Sena Filho et al [15], based on chemical tests, to detect the presence of common secondary metabolite classes, mainly alkaloids, terpenes, and phenolic compounds, in hexane and methanolic extracts, using classical speci�c colour reactions.…”

Section: Methodsmentioning

confidence: 99%

Antitumor Activity of Leaves from Hyptis mutabilis (A. Rich.) Briq. (Lamiaceae) in Mice Bearing Tumor

Ximenes

Melo

Magalhães

et al. 2013

Dataset Papers in Pharmacology

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e Hyptis genus has more than 400 species, many of them being used in folk medicine to treat several conditions. Some anticancer compounds have been isolated from plants of this genus, and for that reason we decided to investigate the potential in vivo antitumor activity of extracts of leaves of Hyptis mutabilis with different polarities (hexane, methanol, water, and hot water) against two mice tumors: sarcoma 180 and Ehrlich solid tumor. Phytochemical analysis revealed strong presence of steroids, saponins, �avonoids (mainly dihydro�avanols), and catechins. Acute toxicity was perfomed according to the up-and-down method showing LD 50 values ranging from 100 up to 2500 mg/kg. Antitumor activity was investigated using 10% of the LD 50 for each extract. Methotrexate was used as positive control. Both aqueous extracts showed strong inhibition of tumor growth with values up to 70% of inhibition growth for sarcoma 180. Ehrlich solid tumor was only slight inhibited by hexane extract (38.6%). In conclusion, the aqueous extracts of H. mutabilis showed promising results against sarcoma 180 mice tumor.

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Antimicrobial activity and phytochemical profile from the roots of Lippia alba (Mill.) N.E. Brown

Cited by 25 publications

References 12 publications

Variabilidade sazonal dos constituintes da própolis vermelha e bioatividade em Artermia salina

Variabilidade sazonal dos constituintes da própolis vermelha e bioatividade em Artermia salina

Arrabidaea chica (HBK) Verlot: phytochemical approach, antifungal and trypanocidal activities

Antitumor Activity of Leaves from Hyptis mutabilis (A. Rich.) Briq. (Lamiaceae) in Mice Bearing Tumor

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