Characterization of the biochemical, physiological, and medicinal properties of Streptomyces hygroscopicus ACTMS-9H isolated from the Amazon (Brazil)

Abstract

Actinomycetes are known to produce numerous secondary bioactive metabolites of pharmaceutical interest. The purpose of this study was to isolate, characterize, and investigate the antibacterial, antifungal, and anticancer activities of metabolites produced by Actinobacteria isolated from the rhizosphere of Paullinia cupana. The Actinobacteria was identified as Streptomyces hygroscopicus ACTMS-9H. Based on a bioguided study, the methanolic biomass extract obtained from submerged cultivation had the most potent antibacterial, antifungal, and cytotoxic activities. This extract was partitioned with n-hexane, ethyl acetate, and 2-butanol. Elaiophylin was isolated from the methanolic biomass extract, and its molecular formula was determined (CHO) based on H andC NMR, IR and MS analyses. The 2-butanol phase was fractionated into four fractions (EB1, EB2A, EB2B, and EB3M). Chemical prospecting indicated the presence of alkaloids, saponins, and reducing sugars in the methanolic extract and 2-butanol phase. The elaiophylin displayed anticancer activity in HEp-2 and HL-60 cells with an IC of 1 μg/mL. The EB1 fraction was selectively toxic to HL-60 cells with IC of 9 ng/mL. Bioautography showed that the EB1 fraction contained an alkaloid with antibacterial and antifungal activities (MIC values ≤1.9 and <3.9 μg/mL, respectively). In conclusion, the EB1 fraction and elaiophylin of S. hygroscopicus have potent antimicrobial, antifungal, and anticancer activities.

Antioxidant, antimicrobial and cytotoxic activities of secondary metabolites from Streptomyces sp. isolated of the Amazon-Brazil region

“…The fermentative process in a solid medium has been shown to be an efficient method to obtain enzymes, antibiotics and other bioactive products, with the advantage of use less water and energy when compared to fermentation in submerged culture (Niladevi, Sukumaran, Prema, 2007). Compared to the results obtained with previous study by Lima et al (2017) using parboiled rice on fermentation in a solid medium, obtained a yield of 6.6%, EMeOH-12H extract showed good productive efficiency, since it presented a percentage of yield above 10%.…”

“…Streptomyces can be found in a diversity of habitats, particularly in the soil and in aquatic or endophytic environments. Besides the antibiotic activity, secondary metabolites produced by Streptomyces are described in the literature as antifungal (Sharma, Manhas, 2020), antiviral (Jakubiec-Krzesniak et al, 2018), anticancer (Tan et al, 2015;Nguyen et l., 2020), antioxidants (Tan et al, 2018) and cytotoxic agents (Lima et al, 2017).…”

Streptomyces sp. JCK-6131 Protects Plants Against Bacterial and Fungal Diseases via Two Mechanisms

“…The ability to enzymatic production, utilization of multiple carbon and nitrogen sources were also evaluated. The physiological tests were performed to estimate the growth of JCK-6131 on different concentrations of NaCl, temperatures as well as pH ( Lima et al, 2017 ).…”

Phân Lập Và Tuyển Chọn Các Chủng Xạ Khuẩn Có Khả Năng Sinh Enzyme Ngoại Bào, Đối Kháng Vibrio Spp.

“…Streptomyces hygroscopicus còn được ứng dụng trong việc tạo ra các cấu tử EB1 và chất elaiophylin có khả năng kháng khuẩn, kháng nấm và có hoạt tính chống ung thư. Trong đó, elaiophylin có tác dụng trên tế bào ung thư HL-60 và HEp-2 với liều IC50 = 1 µg/mL (Lima et al, 2016).…”