-This article carried out the extraction of sesame oil by using three extraction techniques: supercritical fluid extraction (SFE), Soxhlet and sequential extraction. The SFE was performed using supercritical carbon dioxide (SC-CO 2 ) as solvent and ethanol as cosolvent. Tests were performed at 20 MPa, 35°C and a flow rate of 2.5 g CO 2 /min with a total extraction time of 210 minutes. The Soxhlet extraction was performed for 8 hours, using petroleum ether and ethanol as solvents, until the exhaustion of the oil contained in the seeds. The sequential extraction used ethyl ether, ethanol and water as solvents. The Soxhlet extraction was the most effective (58.93%), while the SFE technique obtained 26.47% as the best result. The antioxidant activity (AA) was determined by the β-carotene/linoleic acid system, with good oxidation inhibition percentages (29.32-83.49%) for all the extracts. The main fatty acids (FA) in sesame oil were oleic and linoleic acids.
The bio-residues of Pleurotus ostreatus, Agrocybe cylindracea, and Pleurotus eryingii were studied as sustainable sources of ergosterol. Its extraction was performed by a heat-assisted extraction technique and optimised using response surface methodology. The responses were: extraction yield of the residual material (R) from the mushroom bio-residues dried weight (%), the quantification of ergosterol in the M dw (mg E/100 g M dw), and in the R (mg E/g R). The most feasible responses for industrial transference were obtained for P. ostreatus bio-residues, at the optimal conditions of 65.6 min at 30 • C and 43.7 min at 90 • C producing 43.72 mg E/g R and 290.90 mg E/100 g M dw, respectively. The model satisfactorily fitted the experimental data for all responses, thus implying a good agreement between the experimental values and those predicted by the model. The study proposes a simple and efficient method to produce an ergosterol rich extract from mushroom bio-residues.
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