A novel pre-purification method was developed for producing homoharringtonine from Cephalotaxus koreana, giving high purity and yield. The simple, efficient procedure involved biomass extraction, liquid-liquid extraction, and synthetic adsorbent treatment, followed by low-pressure chromatography. The use of active clay treatment and silica gel low-pressure chromatography in the pre-purification process allowed for the rapid, efficient separation of homoharringtonine from interfering compounds and, compared with alternative processes, increased the yield and purity of crude homoharringtonine for subsequent high-performance liquid chromatography (HPLC) purification. Homoharringtonine of over 52% purity could be obtained simply with high yield from biomass using this pre-purification method, while minimizing solvent use and the scale and complexity of HPLC operations for homoharringtonine purification.
Pedigrees of crop species reflect genealogical relationships among bred lines and are important resources for breeding design. Recently, as more genomic and phenomic data are being accumulated at an astonishing rate, well‐organized pedigrees and database‐linked systems have become ever more important. To cope with the prospective future of breeding science, this study aimed to develop a pedigree‐centered web interface called SoyPedi for the purpose of searching the phenotypic information of soybean [Glycine max (L.) Merr.] cultivars. SoyPedi employed an integrated pedigree composed of 178 well‐defined soybean cultivars and phenotype data. The system was constructed using various bioinformatic tools, such as SQLite3, PHP, and JavaScript. SoyPedi consists largely of three submodules, including a pedigree map, a tabular format database, and single cultivar‐dedicated web pages. The pedigree and tabular databases are interconnected by a single page application system, so that users can promptly switch between these two modules. In addition, comparative analysis of whole genome resequencing‐derived phylogeny and pedigrees implicated some extent of parallel correlation between these two. SoyPedi was developed with the intention of serving breeders for selecting parental lines in hybridization planning, which will be readily operable and intuitive for users. This system will be potentially expandable by integrating more data in the future and is available at https://tgil.donga.ac.kr/SoyPedi/index.html.
This study describes hybrid system of laser with linear slit array generation and aircoupled detection of guided wave in wall thinned pipe. The specimens were produced with elliptical shape in consideration of real shape of wall thinning. These are 2 types with 3 different sizes in terms of depth and width for each type. The characteristic of L(0,1) and L(0,2) depending on the size of depth and width in wall thinned defect is discussed.
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