Development of a pre‐purification process for homoharringtonine

Abstract

A novel pre-purification method was developed for producing homoharringtonine from Cephalotaxus koreana, giving high purity and yield. The simple, efficient procedure involved biomass extraction, liquid-liquid extraction, and synthetic adsorbent treatment, followed by low-pressure chromatography. The use of active clay treatment and silica gel low-pressure chromatography in the pre-purification process allowed for the rapid, efficient separation of homoharringtonine from interfering compounds and, compared with alternative processes, increased the yield and purity of crude homoharringtonine for subsequent high-performance liquid chromatography (HPLC) purification. Homoharringtonine of over 52% purity could be obtained simply with high yield from biomass using this pre-purification method, while minimizing solvent use and the scale and complexity of HPLC operations for homoharringtonine purification.

Inhibitors of Osteoclast Differentiation from Cephalotaxus koreana

“…Supporting Information Available: Physical constant data and references for known compounds (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24) isolated in this study. This material is available free of charge via the Internet at http://pubs.acs.org.…”

“…8 The genus Cephalotaxus is well-known for its alkaloids such as cephalotaxine, harringtonine, and their derivatives, [9][10][11] but few compounds have been reported from C. koreana. 12,13 Amentoflavone-type biflavonoids from the EtOAc-soluble fraction of C. koreana may have therapeutic potential for bone disease. 13 The present study describes the isolation and structure elucidation of five new compounds (1-5) from C. koreana, together with 19 known compounds, and their inhibitory activities toward osteoclast differentiation.…”

Optimal design of a four‐zone simulated moving bed process for separation of homoharringtonine and harringtonine

“…As a mobile‐phase additive, ammonium formate from Samchun Pure Chemical Co. (Seoul, Korea) was added to the mixture of methanol and DDW. The concentration of ammonium formate in the mobile‐phase solution was set at 0.05 M, which was reported to be the optimal concentration leading to the best resolution between HHT and HT (Sung et al, 2005).…”

“…Homoharringtonine (HHT) and harringtonine (HT), which are the alkaloids isolated from the genus Cephalotaxus , have attracted an increasing attention as promising anti‐cancer agents in biochemical industry (Park et al, 1996; Sung et al, 2005). They were also reported to possess anti‐leukaemic activity and their anti‐neoplastic mechanisms have been investigated so far (Park et al, 1996; Sung et al, 2005). Between the two alkaloids, most of clinical testing has been focused on HHT (Beverly et al, 2000).…”

Continuous separation of copper ions from a mixture of heavy metal ions using a three-zone carousel process packed with metal ion-imprinted polymer

“…Chromatographic processes have been widely used in many industries for separation and purification of various systems including a petrochemical mixture or a biochemical mixture [1][2][3]. A matter of primary concern in such applications of chromatographic processes has been the attainment of high separation performance and high economic efficiency [4][5][6].…”